The development of the quick diagnostic method to the avian influenza virus by using latex particle

• Project/Area Number: 18591134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Kyoto Prefectural Institute of Public Health and Environment
• Principal Investigator: ISHIZAKI Tohru Kyoto Prefectural Institute of Public Health and Environment, Kyoto Prefectural Institute for Health and Environment, A chief scientist (60419158)
• Co-Investigator(Kenkyū-buntansha): NAKAYA Takaaki The Research Institute for Microbial Disease, Osaka University, Department of Special Pathogens, Specially apointed associate professor (80271633)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000); Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: Avian Influenza virus / detection method / cross-matching test / monoclonal antibody / Influenza virus new type / Pseudovirus

Research Abstract

1. The purpose of the research To develop an inexpensive, rapid and specific detection system for avian influenza virus A/H5N1(H5N1 Flu)
2. The research programs for the current year (1) To assess the presence of a non-specific response of H5N1-Flu monoclonal antibody latex sensitization particles made in 2006 to influenza virus A(subtypes : A/H1N1, A/H3N2, A/H2N2) and B. (2) To perform a cross matching test between the sensitization particles and other vizuses, e.g., enterovirus, adenovirus, respiratory syncytial virus, mumps virus and herpes simplex virus-1.
3. Results (1) No specific response occurred between the sensitization particles and influenza virus A subtype(A/H1N1,rA/H3N2, A/H2N2) and B. (2) No specific response occurred between the sensitization particles and enterovirus, adenovirus, respiratory syncytial virus, mumps virus and herpes simplex virus-1. (3) The results of the sensitivity experiment which was conducted in the previous year and the cross-matching test performed this year have shown that this research method applying the H5N1-Flu monoclonal antibody latex sensitization particles exhibits a high sensitivity and specificity, and the reaction time was less than 15 minutes. These results suggest that by using this method, the infection by avian influenza virus A/H5N1(HSN1-Flu) can be detected promptly, contributing markedly to the treatment of patients, clarifying the scale of infection, and ensuring public health.