Establishment of model of Influenza encepharopathy in vitro, and analysis of factors for improvement or aggravation in Influenza encepharopathy

• Project/Area Number: 18591161
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fukushima Medical University
• Principal Investigator: HOSOYA Mitsuaki Fukushima Medical University, 医学部, 教授 (80192318)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Yukihiko 福島県立医科大学, 医学部, 准教授 (00305369) ; HASHIMOTO Koichi 福島県立医科大学, 医学部, 講師 (50322342)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,700,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: 小児感染症学 / インフルエンザ脳症 / アポトーシス / 血管内皮細胞 / サイトカイン / 解熱鎮痛剤 / TNF-α / 血清型

Research Abstract

インフルエンザウイルス(FluV)のヒト臍帯静脈血管内皮細胞(HUVEC)への感染とアポトーシス誘導について検討した。FulVはHUVECに感染・増殖しアポトーシスを誘導した。さらにFluV感染はTNF-αによるHUVECのアポトーシスを増強させた。各種解熱鎮痛剤のTNF-α存在下でのHUVECへの影響は、アセトアミノフェンはアポトーシス誘導、生細胞数ともにコントロールと同レベルであった。一方、インドメタシン、ジクロフェナックは濃度依存的に細胞毒性を示した。以上の結果は、現在有効な治療法の確立されていないインフルエンザ脳症の病態を明らかにすると同時に、有効な予防・治療法確立の一助になると思われる。

Research Products

• [Journal Article] Human influenza virus infection and apoptosis induction in human vascular endothelial cells (2008)
  • Author(s): Sumikoshi M, Hashimoto K, Kawasaki Y(他4名, 最終共著者)
  • Journal Title: J Med Virol 80(6) Pages: 1072-1080
  • Peer Reviewed
• [Journal Article] Human influenza virus infection and apoptosis induction in human vascular endothelial cells (2008)
  • Author(s): 隅越 誠
  • Journal Title: Journal of Medical Virology 80 Pages: 1072-1078
  • Peer Reviewed
• [Journal Article] Human influenza virus infection and apoptosis induction in human vascular endothelial cells (2008)
  • Author(s): 隅越 誠
  • Journal Title: Journal of Medical Virology (In press)
  • Peer Reviewed
• [Journal Article] Genetic diversity of coxsackievirus A16 associated with hand, foot, and mouth diseaseepidemics in Japan from 1983 to 2003 (2007)
  • Author(s): Hosoya M, Kawasaki Y, Sato M(他6名)
  • Journal Title: J Clin Microbiol 45(1) Pages: 112-20
  • Peer Reviewed
• [Journal Article] Genetic diversity of coxsakievirus A16 associated with hand, foot. and mouth disease epidemics in Japan from 1983 to 2003 (2007)
  • Author(s): 細矢光亮
  • Journal Title: J clin Microbiol 45・1 Pages: 112-120
• [Journal Article] Genetic diversity of enterovirus 71 associated with hand, foot and mouth disease epidemics in Japan from 1983 to 2003 (2006)
  • Author(s): Hosoya M, Kawasaki Y, Sato M(他5名)
  • Journal Title: Pediatr Infect Dis J 25(8) Pages: 691-4
  • Peer Reviewed
• [Journal Article] Prognstic predictive values of serum cytochrome c, cytokines, and other laboratory measurements in acute encephalopathy with multiple organ failure (2006)
  • Author(s): Hosoya M, Kawasaki Y, Katayose M(他7名)
  • Journal Title: Arch Dis Child 91(6) Pages: 469-472
  • Peer Reviewed
• [Journal Article] Prognostic predictive values of serum cytochrome c, cytokines, and other laboratory measurements in acute encephalopathy with multiple organ failure (2006)
  • Author(s): 細矢光亮
  • Journal Title: Arch Dis Child 91・6 Pages: 469-472
• [Journal Article] Genetic diversity of enterovirus 71 associated with hand, foot and mouth disease epidemics in Japan from 1983 to 2003 (2006)
  • Author(s): 細矢光亮
  • Journal Title: Pediatr Infect Dis J 25・8 Pages: 691-694