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Roles of leukotrienes in the development of wheeze in infants

Research Project

Project/Area Number 18591192
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionSaga University

Principal Investigator

HAMASAKI Yuhei  Saga University, Pediatrics, Professor (10172967)

Co-Investigator(Kenkyū-buntansha) TASHIRO Katsuya  Saga University, Pediatrics, Assistant Professor (70295046)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsWheeze / leukotriene / viral infection in respiratory tract
Research Abstract

The aims of this study are (1) to clarify how environmental factors are involved in the development of virus-induced wheezing in infants, and(2) to elucidate how these factors influence arachidonic acid-metabolism and receptors of these metabolites in these subjects. We also investigate whether asthma development can be inhibited by early use of certain anti-inflammatory drugs such as inhaled steroids and leukotoriene receptor antagonists.
We have established the cultured cell lines of human tracheobronchial cells including normal cells. We found that these tracheobronchial cells express receptors of cysteinyl leukotrienes (cysLTR1 and cysLTR2) and leukotrieneB4 (BLT). By using these cell lines, we have conducted the following experiments.
We firstly transfected artificially synthesized poly inosinic-cytidyric acid: poly IC (dsRNA) into these cells as a model of virus infection. Transfection of poly IC strongly induced the expression of mRNA and protein synthesis of RANTES, IL-8 and eotaxin-3. We also found that the expression of mRNA and protein synthesis of eotaxin 3 is dose- and time-dependently increased by addition of IL-4 and IL-13, Th2 cytokines. When cells are stimulated with IL-4 after 24 hour-incubation of dsRNA, the induction of eotaxin 3 was additively increased. The mechanism of this enhancement is a new induction of the molecules of IL+C257-4 receptors (IL-4Ra and IL-2Rr) by dsRNA. We found that the production of eotaxin 3 is further increased in the presence of cysteinyl leukotrien and inhibited by leukotriene receptor antagonist, indicating that viral infection of respiratory tract and cysteinyl leukotrienes is associated with the development of wheezing in infants.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (22 results)

All 2007 2006

All Journal Article (15 results) (of which Peer Reviewed: 6 results) Presentation (6 results) Book (1 results)

  • [Journal Article] 気道上皮とメディエーター2007

    • Author(s)
      山本 修一、浜崎 雄平
    • Journal Title

      臨床免疫・アレルギー科 47

      Pages: 504-510

    • NAID

      40015482723

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] uric acid transporter 1 gene analysis in familial renal hypo-uricemia associated with exercise-induced acute renal failure2007

    • Author(s)
      Ohtuka Y, Zaitsu Y, Ichida K, Isomura N, Tsuji K, Sato T, Hamasaki Y
    • Journal Title

      Pediatric International 49

      Pages: 235-237

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 喘息におけるLT拮抗薬の位置づけ2007

    • Author(s)
      浜崎 雄平
    • Journal Title

      小児内科 39

      Pages: 835-837

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] 気道上皮とメディエーター2007

    • Author(s)
      山本 修一, 浜崎 雄平
    • Journal Title

      臨床免疫・アレルギー科 47(4)

      Pages: 504-510

    • NAID

      40015482723

    • Related Report
      2007 Annual Research Report
  • [Journal Article] uric acid transporter 1 gene analysis in familial renal hypo-uricemia associated with exercise-induced acute renal failure2007

    • Author(s)
      Ohtuka Y, Zaitsu Y, Ichida K, Isomura N, Tsuji K, Sato T, Hamasaki Y,
    • Journal Title

      Pediatric International 49

      Pages: 235-237

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-18 primes human basophilic KU812 cells for higher leukotriene synthesis2006

    • Author(s)
      Zaitsu M, Yamasaki F, Hamasaki Y
    • Journal Title

      Prostaglandins Leukotriene Essential Fatty Acids 74

      Pages: 61-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased IL-1 production from dsRNA-stimulated leukocytes in febrile seizures2006

    • Author(s)
      Matsuo M, Sasaki K, Hamasaki Y
    • Journal Title

      Pediatr Neurol 35

      Pages: 102-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2本鎖DNA(dsDNA)による気道上皮のIL-4レセプター発現増強作用2006

    • Author(s)
      西奈 津子, 辻 功介, 山本 修一, 浜崎 雄平
    • Journal Title

      臨床・免疫・アレルギー科 46

      Pages: 310-313

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression and production of aberrant Pax5 with deletion of exon-8 in B-lineage acute lymphoblastic leukemia of children2006

    • Author(s)
      Sadakane Y, Zaitsu M, Hamasaki Y
    • Journal Title

      Brit J Haematol 136

      Pages: 297-300

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Superoxide production in human neutrophils is enhanced by treatment of transmembrane peptides derived from human formyl peptide receptor2006

    • Author(s)
      Hayashi S, Osada M, Hamasaki Y, et. al.
    • Journal Title

      J Biochem 130

      Pages: 102-106

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Superoxide production in human neutrophils is enhanced by treatment of transmembrane peptides derived from human formyl peptide receptor. J. Biochem.2006

    • Author(s)
      R., Hayashi, S., Osada, M., Yoshiki, D., Sugiyama, I., Fujita, Y., Hamasaki, H., Kodama
    • Journal Title

      130

      Pages: 981-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Increased IL-1. production from dsRNA-stimulated leukocytes in febrile seizures2006

    • Author(s)
      Matsuo, M., Sasaki, K., Ichimaru, T., Nakazato, S., Hamasaki, Y
    • Journal Title

      Pediatr Neurol 35

      Pages: 102-106

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interleukin-18 primes human basophilic KU812 cells for higher leukotriene synthesis.2006

    • Author(s)
      Zaitsu, M., Yamasaki, F., Ishii, E., Midoro-Horiuti, T., Goldblum, RM., Hamasaki, Y
    • Journal Title

      Prostaglandins Leukot Essent Fatty Acids 74(1)

      Pages: 61-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 2本鎖DNA(dsDNA)による気道上皮のIL-4レセプター発現増強作用2006

    • Author(s)
      西奈津子, 辻功介, 山本修一, 浜崎雄平
    • Journal Title

      臨床・免疫・アレルギー科 46

      Pages: 310-313

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Superoxide production in human neutrophils is enhanced by treatment of transmembrane peptides derived from human formyl peptide receptor2006

    • Author(s)
      Hayashi S, Osada M, Hamasaki Y, et al.
    • Journal Title

      J Biochem 130

      Pages: 102-106

    • Related Report
      2006 Annual Research Report
  • [Presentation] 気道上皮細胞におけるIFN-βによるRANTES産生についての検討2007

    • Author(s)
      室 英理子, 浜崎 雄平, 他
    • Organizer
      第19回日本アレルギー学会春季臨床大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-06-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 気道上皮細胞におけるケモカイン産生を制御する因子2007

    • Author(s)
      西奈 津子、浜崎 雄平
    • Organizer
      第44回日本小児アレルギー学会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genistein,a soybean derived isoflavonoid,inhibits leukotriene C4 synthesis in Rat Basophilic Leukemia-2H3 cells2006

    • Author(s)
      Yuhei Hamasaki
    • Organizer
      Asia Pacific Association and Clinical Immunology, Respirology and Immunology
    • Place of Presentation
      Jakarta
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ヒト培養気道上皮細胞におけるLTD4のケモカイン産生に対する作用2006

    • Author(s)
      西奈 津子、欧 維林、在津 正文、室 英理子、山本 修一、浜崎 雄平
    • Organizer
      第18回日本アレルギー学会春季臨床大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] KeratinocyteはCysLT1受容体を発現する2006

    • Author(s)
      西奈 津子、辻 功介、宮崎 倫子、人見 知洋、在津 正文、室 英理子、稲田成安、小林 育子、山本 修一、市丸 智浩、浜綺 雄平
    • Organizer
      第56回日本アレルギー学会秋季学術大会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Genistein, a soybean derived isoflavonoid, inhibits leukotriene C4 synthesis in Rat Basophilic Leukemia-2H3 cells.2006

    • Author(s)
      Yuhei, Hamasaki
    • Organizer
      Asia Pacific Association and Clinical Immunology(Abstract 61)
    • Place of Presentation
      Respirology and Immunology Jakarta
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 免疫学コア講義改訂2版2007

    • Author(s)
      浜崎 雄平
    • Total Pages
      8
    • Publisher
      南山堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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