Establishment of the novel and basic technique for inducing human ES cells to differentiate into connective tissue-type mast cells and their utilization in clinical practice
| Project/Area Number |
18591217
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MA Feng The University of Tokyo, The Institute of Medical Science, Business-Academia-Government CollaborationRese (20378748)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Human embryonic stem cells / Mast cells / Hematopoietic progenitors / Chymase / Tryptase / Connective tissue-type mast cells / Mucosal mast cells / Stroma / ヒト胚性幹細胞(ES細胞) / 胎仔肝 / ストローマ細胞 / 多能性造血細胞 / 組織型肥満細胞 / アレルギー |
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| Research Abstract |
In human being, mast cells (MC) play a central role in innate immune and function as cellular mediators of allergy. During infancy, the establishment of innate immuno-system is largely based on the encountering and recognizing of new antigens. Since MCs are typically located at strategically important body barriers, such as skin, vascular and mucosal tissues, they are one of the first reactors in the developmental immunes in infants. However, most of MC data derive solely from experiments in mice and rats. Human MC data are very limited. A particular problem of human MC research is the difficulty in obtaining human material for in vitro studies.
I have already established method to generate fuctional MCs from non-human primate ES cells, as reported above. More recently, by the financial supporting from JSPS, we have successfully established a similar method to produce quantitative functionally mature mast cells from hESCs, providing a large variety of possibilities in research on human MC development and their functions. In the present study, I found hESC-derived MC development might be along to two pathpawys : At primary time of induction, they first develop CT-MC that both express chymase and tryptase, and then from multipotential hematopoietic progenitor cells at a comparatively later stage they develop M-MC. This is quite similar to what we have found in monkey ESC-derived MC development, suggesting a unique pathway of MC development during early embryonic/fetal stages is common in human and non-human primates. It also offers a good model to investigate the first establishment of innate immune system mediated by human MCs in infants. Our finding is so far the first report of human/non-human primate ES derived functional MCs in the world, and should open a new research field on MC development.
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Report
(3 results)
Research Products
(30 results)
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[Journal Article] α4-Integrin+ endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells2007
Author(s)
G.Shinoda, K.Umeda, T.Heike, M Arai, A.Niwa, F.Ma, H.Suemori, H.Y.Luo, D.H.K.Chui, R.Torii, M.Shibuya, Nakatsuji, T.Nakahata
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Journal Title
Blood 109
Pages: 2406-2415
Related Report
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[Journal Article] Sequential analysis of α- and β-globin gene expression during erythropoietic differentiation from primate embryonic stem cells2006
Author(s)
K.Umeda, T.Heike, M.Nakata-Hizume, A.Niwa, M.Arai, G.Shinoda, F.Ma, H.Suemoni, H.Y.Luo, D.H.K.Chui, R.Torii, M.Shibuya, N.Nakatsuji, T.Nakahata
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Journal Title
Stem Cells 24
Pages: 2627-2636
Related Report
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