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The role of calcium-responsive element located in human keratin 1 gene on keratinocyte differentiation

Research Project

Project/Area Number 18591232
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionUniversity of Tsukuba

Principal Investigator

OTSUKA Fujio  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Professor (10092157)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,110,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordskeratin 1 / keratinocyte / keratinization / ケラチン1 / 分化
Research Abstract

The mammalian epidermis is a constantly renewing stratified squamous epithelium. The major cell type of the epidermis is the epidermal keratinocyte. Keratinocytes undergo a strictly regulated, highly compartmentalized differentiation process that results in a mature stratified epidermis. The basal layer of the epidermis contains proliferating keratinocytes. In response to differentiating signals, these cells stop dividing and simultaneously mature and migrate through the spinous and granular layers of the epidermis. Ultimately, they are transformed into flattened squamas and sloughed into the environment. Expression of the Keratin 1 (K1) gene is tightly regulated and is coincident with a distinct differentiation state of keratinocytes. Transcription of K1 is primarily localized to the spinous layer. Evaluation of approximately 6 kb of 5' franking sequence indicated that these sequences possessed the information required for tissue-and differentiation-specific expression. Investigation of distal sequences identified Sp 1 site. Mutation of either of these sites, which ablated binding, resulted in significant decreases in promoter activity. Sequences containing the distal element, while required for high levels of expression, were responsible for significant suppression in the presence of Sp1. Collectively, these data identify and characterize several regulatory elements that cooperate to cause the appropriate tissue-, developmental-, and differentiation-specific expression of the hK1 gene.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (25 results)

All 2008 2007 2006 Other

All Journal Article (22 results) (of which Peer Reviewed: 12 results) Presentation (3 results)

  • [Journal Article] Deep cutaneotis infeetion by Fusarium solani in a healthy child: successful treatment with local heat therapy2007

    • Author(s)
      Y Nakamura, F Otsuka, et.al
    • Journal Title

      Journal of Ameriean Academy ofDermatology 56

      Pages: 873-877

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Thl and type 1 eytotoxic T cells dominate the responses lnt-bet overexpression transgenic mice that develop contaet dermatitis"2007

    • Author(s)
      K Ishizaki, F Otsuka, et. al.
    • Journal Title

      Journal of Immuno logy 178

      Pages: 605-612

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] IgA/IgG pemphiguS positive forl anti-desmoglein 1,autoant ibody.2007

    • Author(s)
      H Maruyama, F Otsuka, et. al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 94-95

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cutaneous deep necrosis with dermatomyositis: correlation ith interstitial Pneumonia.2007

    • Author(s)
      Kawa chi Y, Otsuka F, et. al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 345-346

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The combination・of ubiquitous.transcription factors AP-1 and Spl directs keratinocyte-specific and differentiation- specific gene expression in vitro.2007

    • Author(s)
      Nakamura Y, Otsuka F, et. al.
    • Journal Title

      Expimental Dermatology 16

      Pages: 143-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Epidermal cell necrosis with direct epidermal infiltration bf EB-ER-positive T 1ymphocytes in a patient with Epstein-Bar-virus-associated hemophagecytie syndrome."2007

    • Author(s)
      Kawa chi Y, Otsuka F, et. al.
    • Journal Title

      British Journal of Dermatology 157

      Pages: 1053-1056

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Deep cutaneous infection by Fusarium solani in a healthychild : successful treatment with local heat therapy2007

    • Author(s)
      Y Nakamura, F Otsuka, et. al.
    • Journal Title

      Journal of American Academyof Dermatology 56

      Pages: 873-877

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Th1 and type 1 cytotoxic T cells dominate the responses int-bet overexpression transgenic mice that developcontact dermatitis2007

    • Author(s)
      K Ishizaki, F Otsuka, et. al.
    • Journal Title

      Journal of Immunology 178

      Pages: 605-612

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] IgA/IgG pemphigus positive for anti-desmoglein 1 autoantibody2007

    • Author(s)
      H Maruyama, F Otsuka, et. al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 94-95

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cutaneous deep necrosis with dermatomyositis : correlation withinterstitial pneumonia2007

    • Author(s)
      Kawachi Y, Otsuka F, et. al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 345-346

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The combination of ubiquitous transcription factors AP-1 and Sp1 directs keratinocyte-specific and differentiation-specific gene expression in vitro2007

    • Author(s)
      Nakamura Y, Otsuka F, et. al.
    • Journal Title

      Expimental Dermatology 16

      Pages: 143-150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Epidermal cell necrosis with direct epidermal infiltration of EB-ER-positive T lymphocytes in a patient with Epstein-Barrvirus-associated hemophagocytic syndrome2007

    • Author(s)
      Kawachi Y, Otsuka F, et. al.
    • Journal Title

      British Journal of Dermatology 157

      Pages: 1053-1056

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Deep cutaneous infection by Fusarium solani in a healthy child: successful treatment with local heat therapy.2007

    • Author(s)
      Y Nakamura, F Otsuka, et. al.
    • Journal Title

      Journal of American Academy of Dermatology 56

      Pages: 873-877

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Th1 and type 1 cytotoxic T cells dominate the responses in t-bet overexpression transgenic mice that develop contact dermatitis.2007

    • Author(s)
      K Ishizaki, F Otsuka, et. al.
    • Journal Title

      Journal of Immunology 178

      Pages: 605-612

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IgA/IgG pemphigus positive for anti-desmoglein 1 autoantibody.2007

    • Author(s)
      H Maruyama, F Otsuka, et. al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 94-95

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous deep necrosis with dermatomyositis: correlation with interstitial pneumonia.2007

    • Author(s)
      Kawachi Y, Otsuka F, et. al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 345-346

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The combination of ubiquitous transcription factors AP-1 and Sp1 directs keratinocyte-specific and differentiation-specific gene expression in vitro.2007

    • Author(s)
      Nakamura Y, Otsuka F, et. al.
    • Journal Title

      Expimental Dermatology 16

      Pages: 143-150

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epidermal cell necrosis with direct epidermal infiltration of EB-ER-positive T lymphocytes in a patient with Epstein-Barr-virus-associated hemophagocytic syndrome.2007

    • Author(s)
      Kawachi Y, Otsuka F, et. al.
    • Journal Title

      British Journal of Dermatology 157

      Pages: 1053-1056

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Th1 and type 1 cytotoxic T cells dominate the responses in t-bet overexpression transgenic mice that develop contact dermatitis.2007

    • Author(s)
      K Ishizaki, F Otsuka, et al.
    • Journal Title

      Journal of Immunology 178

      Pages: 605-612

    • Related Report
      2006 Annual Research Report
  • [Journal Article] IgA/IgG pemphigus positive for anti-desmoglein 1 autoantibody.2007

    • Author(s)
      H Maruyama, F Otsuka, et al.
    • Journal Title

      European Journal of Dermatology 17

      Pages: 94-95

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Giant cell tumor of the distal phalanx of the foot.2006

    • Author(s)
      Y Fujisawa, F Otsuka, et al.
    • Journal Title

      European Journal of Dermatology 16

      Pages: 9-11

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Deep cutaneous infection by Fusarium solani in a healthy child : successful treatment with local heat therapy.

    • Author(s)
      Y Nakamura, F Otsuka, et al.
    • Journal Title

      Journal of American Academy of Dermatology (in press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] 膠原線維アクック型反応が見られた皮膚筋炎2008

    • Author(s)
      古田淳一、川内康弘、大塚藤男
    • Organizer
      第31回皮膚脈管膠原病研究会
    • Place of Presentation
      前橋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A case of dermatomyositis presented the collagen fiber attack type reaction2008

    • Author(s)
      J Furuta, F Otsuka, et. al.
    • Organizer
      The 31st Annual Meeting of The Society for Cutaneous Veneology and Collagen Diseases
    • Place of Presentation
      Maebashi
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 膠原線維アタック型反応が見られた皮膚筋炎2008

    • Author(s)
      古田淳一、川内康弘、大塚藤男
    • Organizer
      第31回皮膚脈管膠原病研究会
    • Place of Presentation
      前橋
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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