| Project/Area Number |
18591240
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kanazawa University |
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Principal Investigator |
TAKEHARA Kazuhiko Kanazawa University, Graduate Shcool of Meclical Science, Professor (50142253)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Manabu Kanazawa University, Graduate School of Medicine Sience, Associate Professor (90272591)
CHUJO Sonoko Kanazawa University, University Hospital, Assistant Professor (20401911)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | fibrosis / collagen / TGF-β / CTGF / monoclonal antibody / 皮膚生理学 |
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| Research Abstract |
We established skin fibrosis animal model by subcutaneous injection of two cytokines, TGF-β and CTGF. We have conducted this project to inhibit this fibrosis model by anti-CTGF antibody.
We succeeded in establishment of anti-human CTGF module 1, 2, 3 and 4 antibodies by DNA immunization method of human CTGF vector and gene gun. After the several screening studies, the anti-human CTGF module 2 antibody was revealed to be most potent of neutralizing activity.
The anti-human CTGF antibody inhibited skin fibrosis dramatically, both in the early phase of TGF-β injection and the late phase of CTGF injection. In this experiment, this anti-human CTGF antibody inhibited mouse internal CTGF action.
In the future, we are planning to create humanized anti-CTGF antibody for the clinical application.
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