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Identification and differentiation of Merkel stem cells or precursor cells

Research Project

Project/Area Number 18591252
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSaga University

Principal Investigator

NARISAWA Yutaka  Saga University, Saga Medical School, Professor (60164498)

Co-Investigator(Kenkyū-buntansha) MISAGO Noriyuki  Saga University, Saga Medical School, Assistant professor (90199977)
INOUE Takuya  Saga University, Saga Medical School, Associate professor (50380754)
OOKAWA Takeshi  Saga University, Saga Medical School, Assistant (90418814)
KOBE Shnichi  Saga University, Saga Medical School, Assistant (60418811)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsMerkel cell / stem cell / precursor cell / コラーゲンゲル培養法 / 表皮シート / 培養 / 気相-液相界面微小環境 / ケラチノサイト
Research Abstract

Merkel cells exist in the basal layer of the epidermis, more especially at the bottom of the rete ridges, contacting with keratinocytes. We thus hypothesized that a direct contact between Merkel cells and keratinocytes may be relevant to the maintenance of Merkel cell survival and function. To address our hypothesis, we developed a new organotypic culture system of Merkel cells, using Merkel cell-containing epidermal sheets. This culture system maintains Merkel cell survival within epidermal rete ridges for more than 2 weeks without neurotrophic growth factors (neurotrophin-3 and nerve growth factor). Coculture of Merkel cells with nerve cells (PC12) promoted Merkel cell survival within epidermal sheets, in which keratinocytes replicated cytokeratins (CK1, CK10, CK14) and p63 expression of in vivo keratinocytes. Merkel cell proliferation was not detected in all conditions. The data suggest, firstly, that a direct contact between Merkel cells and keratinocytes is one of the critical factors in Merkel cell survival ; secondly that Merkel cells may be a terminally differentiated cell type. Our culture system will open up a new way to study Merkel cell biology.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report

Research Products

(4 results)

All 2007

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Merkel cell-poor trichoblastoma with basal cell carcinoma-like foci2007

    • Author(s)
      Misago N, Satoh T, Miura Y, Nagase K, Narisawa Y.
    • Journal Title

      Am J Dermatopathol 29

      Pages: 249-255

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Merkel cell-poor trichoblastoma with basal cell carcinoma-like foci2007

    • Author(s)
      Misago, N., Satoh, T., Miura, Y., Nagase, K., Narisawa, Y
    • Journal Title

      Am J Dermatopathol 29

      Pages: 249-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] メルケル細胞の表皮シートを用いた新規培養法の確立2007

    • Author(s)
      永瀬 浩太郎、成澤 寛、内橋 和芳、青木 茂久、戸田 修二
    • Organizer
      第11回九州基礎皮膚科研究会
    • Place of Presentation
      福岡市
    • Year and Date
      2007-12-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Establishment of a new culture system of Merkel cells using epidermal sheett2007

    • Author(s)
      Nagase, K., Narisawa, Y., Uchihashi, K., Aoki, S., Toda, S
    • Organizer
      11th Meeting of Kyushu Basic Dermatological Research
    • Place of Presentation
      Fukuoka
    • Year and Date
      2007-12-08
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-03-31   Modified: 2016-04-21  

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