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micro RNAs and maintenance of keratinocyte functions

Research Project

Project/Area Number 18591266
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionHyogo College of Medicine

Principal Investigator

YAMANISHI Kiyofumi  Hyogo College of Medicine, Faculty of Medicine, Professor (10182586)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsskin / epidermis / keratinocytes / gene regulation / RNA / microarray / マイクロアレイ / mRNA
Research Abstract

MicroRNAs (miRNA) are functional RNAs that regulate gene expression in groups. Recently, miRNAs have been supposed to be involved in pathophysiology of a variety of diseases. In the present study, we have focused on the role of miRNAs in keratinocyte growth and differentiation and on the regulation of downstream genes. When cultured human keratinocytes were incubated in a high Ca2+ condition, a screening using mircroarray revealed that the expression of several miRNAs including hsa-let7b and hsa-miR-29a was altered. The changes were confirmed by real-time quantitative PCR. The transfection of hsa-miR-29a into keratinocytes significantly suppressed the expression of several genes including transglutaminase 1 gene (TGM1). Lacking TGM in mice leads to the changes in the expression of the genes for cholesterol metabolism and a serine protease in the epidermis, and therefore, has-miR-29a regulates those genes upstream to maintain keratinocyte cellular functions.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006

All Journal Article (15 results) (of which Peer Reviewed: 5 results) Presentation (3 results) Book (2 results)

  • [Journal Article] The effect of 14-membered ring macrolides on the production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes2008

    • Author(s)
      Tatsuya Tsuda
    • Journal Title

      Antimicrobial Agents and Chemotherapy 52

      Pages: 1538-1541

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The effect of 14-membered ring macrolides on the production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes.2008

    • Author(s)
      Tsuda T, Ishikawa C, Konishi H, Hayashi Y, Nakagawa N, Matsuki M, Mizutani H, Yamanishi K.
    • Journal Title

      Antimictob Agents Chemother 52

      Pages: 1538-1541

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The effect of 14-membered ring macrolides on the production of interleukin-8mediated by protease activated receptor 2 in human keratinocytes2008

    • Author(s)
      Tatsuya Tsuda
    • Journal Title

      Antimicrobial Agents and Chemotherapy 52

      Pages: 1538-1541

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Staphylococcal enterotoxin B enhances a flare-up reaction of murine contacthypersensitivity through up-regulation of interferon-gamma2008

    • Author(s)
      Akiko Miyata
    • Journal Title

      Experimental Dermatology

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ここまで分かった角化異常と皮膚のバリア障害2007

    • Author(s)
      山西 清文
    • Journal Title

      日本皮膚科学会雑誌 117

      Pages: 2310-2311

    • NAID

      10027062750

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Contribution of interleukin-18 to atopic dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice2006

    • Author(s)
      Makoto Terada
    • Journal Title

      Proceedings of the National Academy of Science, USA 103

      Pages: 8816-8821

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment2006

    • Author(s)
      Yohichi Omoto
    • Journal Title

      Journal of Immunology 177

      Pages: 8315-8319

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment.2006

    • Author(s)
      Omoto Y, Tokime K, Yamanaka K, Habe K, Morioka T, Kurokawa I, Tsutsui H, Yamanishi K, Nakanishi, K, Mizutani H.
    • Journal Title

      J Immunol 177

      Pages: 8315-8319

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Contribution of interleukin-18 to atopic dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice.2006

    • Author(s)
      Terada M, Tsutsui H, Yasutomo I, Yasuda K, Mizutani H, Yamanishi K, Kubo M, Matsui K, Sano H, Nakanishi K.
    • Journal Title

      Proc Natl Acad Sci USA 103

      Pages: 8816-8821

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Contribution of interleukin-18 to atopic dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice.2006

    • Author(s)
      Makoto Terada
    • Journal Title

      Proceedings of the National Academy of Science,USA 103・23

      Pages: 8816-8821

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment.2006

    • Author(s)
      Yohichi Omoto
    • Journal Title

      Journal of Immunology 177・12

      Pages: 8315-8319

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 男性型脱毛症・女性型脱毛症 -PHL治療薬のEBM-2006

    • Author(s)
      山西清文
    • Journal Title

      皮膚病診療 28・4

      Pages: 400-405

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 角質バリアの形成と破綻2006

    • Author(s)
      山西清文
    • Journal Title

      角化症研究会記録集 20

      Pages: 70-77

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 乾癬とカリクレイン2006

    • Author(s)
      山西清文
    • Journal Title

      日本乾癬学会記録集 21

      Pages: 107-108

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Non-anogenital (ectopic) hidradenoma papilliferum with sebaceous differentiation : a case report and review of reported cases2006

    • Author(s)
      Shoichiro Minami
    • Journal Title

      Journal of Dermatology 334・256

      Pages: 256-259

    • Related Report
      2006 Annual Research Report
  • [Presentation] Functional genomic analysis of skin barrier defects with deranged molecular organization of stratum corneum intercellular lipids in mice lacking transglutaminase 12007

    • Author(s)
      Noboru Nakagawa
    • Organizer
      13th European Congress on Biotechnology
    • Place of Presentation
      バルセロナ,スペイン
    • Year and Date
      2007-09-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Functional genomic analysis of skin barrier defects with deranged molecular organization of stratum corneum intercellular lipids in mice lacking teansglutaminase 12007

    • Author(s)
      Noboru Nakagawa
    • Organizer
      13th European, Congress on Biotechnology
    • Place of Presentation
      バルセロナ、スペイン
    • Year and Date
      2007-09-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] Functional genomic analysis of skin barrier defects with deranged molecular organization of stratum corneum intercellular lipids in mice lacking transglutaminase 1.2007

    • Author(s)
      Nakagawa N, Tsuda T, Ohta N, Matsuki M, Yagi N, Hatta I, Tsujimoto G, Yamanishi K.
    • Organizer
      13th European congress on Biotechnology
    • Place of Presentation
      Barcelona, Spain
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 今日の治療指針2007

    • Author(s)
      山西 清文
    • Total Pages
      849
    • Publisher
      医学書院
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Book] 先端医療シリーズ38 皮膚疾患の最新医療2006

    • Author(s)
      斎田俊明
    • Total Pages
      318
    • Publisher
      先端医療技術研究所
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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