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Establishment of murine model of eosinophil-infiltrating drug photoallergy and elucidation of its immunological mechanism

Research Project

Project/Area Number 18591267
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

TOKURA Yoshiki  University of Occupational and Environmental Health, Japan, Department of Dermatology, Professor and Chairman (00172156)

Co-Investigator(Kenkyū-buntansha) KABASHIMA Kenji  University of Occupational&Environmental Health, Department of Dermatology, Associate Professor (00362484)
KAZUNARI Sugita  University of Occupational&Environmental Health, Department of Dermatology, Senior Resident (40412647)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,750,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsdrug / photosensitivity / ultraviolet light / eosinophil / lymphocyte
Research Abstract

We established a murine model of the eosinophil-infiltrating type of drug photoallergy by using afloqualone (AQ), a representative photosensitive drug. AKR/J mice were sensitized by an i.p. injection of afloqualone solution and irradiation of shaved abdomen with UVA. This sensitization procedure was repeated 2-12 times (twice a week), and 3 days after the last immunization, mice were challenged by an s.c. injection of AQ solution and irradiation of the same site with UVA. Mice receiving more than 10 times of sensitization exhibited a massive infiltrate of eosinophils and lymphocytes at the challenged site. This reaction was AQ photoantigen-specific, and AKR/J mice were a high responder strain of this sensitivity. The sensitivity was successfully transferred with 5-8x10^7 immune lymph node and spleen cells into naive mice. CD4^+ T cells were responsible for this sensitivity, since 1x10^7 CD4^+ cells alone induced a high level of sensitivity reaction and the addition of other lymphocyte populations did not enhance the reaction. Culture supernatants from immune lymph node cells contained a higher level of IL-4 and a lower level of interferon-g than those from naive mice. Finally, the skin of AQ-photochallenged site exhibited high expression of CCL24/eotaxin-2, a chemokine for eosinophils. It is suggested that eosinophilic drug photoallergy is medated by sensitized Th2 cells and locally produced eosinophil-attracting chemokines. This model is useful for exploring the mechanism of eosinophil-infiltrating photosensitivity.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (58 results)

All 2008 2007 2006

All Journal Article (41 results) (of which Peer Reviewed: 17 results) Presentation (9 results) Book (8 results)

  • [Journal Article] Epidermal chemokines and modulation by antihistamines, antibiotics and antifungals.2008

    • Author(s)
      Tokura Y
    • Journal Title

      Exp Dermatol 17

      Pages: 81-90

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Cutaneous Hypersensitivities to Hapten Are Controlled by IFN-gamma-Upregulated Keratinocyte Th 1 Chemokines and IFN- gamma-Downregulated Langerhans Cell Th2 Chemokines.2008

    • Author(s)
      Mon T
    • Journal Title

      J Invest Dermatol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Alterations of serum Thi and Th2 chemokines by combination therapy of interferon-gamma and narrowband UVB in patients with mycosis fungoides.2008

    • Author(s)
      Shimauchi T
    • Journal Title

      J Dermatol Sci (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Phototoxic and photoallergic reactions.2008

    • Author(s)
      Tokura Y
    • Journal Title

      Drug Discovery Today (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Induction of keratinocyte apoptosis by photosensitizing chemicals plus UVA2007

    • Author(s)
      Kurita M
    • Journal Title

      J Dermatol Sci 45

      Pages: 105-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prostaglandin E(2) is required for ultraviolet B-induced skin inflammation via EP2 and EP4 receptors2007

    • Author(s)
      Kabashima K
    • Journal Title

      Lab Invest 87

      Pages: 49-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] CXCL12-CXCR4 engagement is required for migration of cutaneo us dendritic cells2007

    • Author(s)
      Kabashima K
    • Journal Title

      Am J Pathol 171

      Pages: 1249-1257

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] CXCR4 engagement promotes dendritic cell survival and maturation2007

    • Author(s)
      Kabashima K
    • Journal Title

      Biophys Res Commun 361

      Pages: 1012-1016

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inhibition of T helper 2 chemokine production by narrowband ultraviolet B in cultured keratinocytes2007

    • Author(s)
      Hino R
    • Journal Title

      Br J Dermatol 156

      Pages: 830-837

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Stimulation of Langerhans cells with ketoprofen plus UVA in murine photocontact dermatitis to ketoprofen2007

    • Author(s)
      Atarashi K
    • Journal Title

      J Dermatol Sci 47

      Pages: 151-159

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] T cell populations populating in the peripheral blood of patients with drug eruptions2007

    • Author(s)
      Nishio D
    • Journal Title

      J Dermatol Sci 48

      Pages: 25-33

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 光アレルギーの発症機序と対策2007

    • Author(s)
      戸倉 新樹
    • Journal Title

      日本内科学雑誌 96

      Pages: 146-152

    • NAID

      10019986332

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Induction of keratinocyte apoptosis by photosensitizingchemicals plus UVA.2007

    • Author(s)
      Kurita M
    • Journal Title

      J Dermatol Sci 45

      Pages: 105-112

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prostaglandin E(2) is required for ultravioletB-induced skin inflammation via EP2 and EP4 receptors.2007

    • Author(s)
      Kabashima K
    • Journal Title

      Lab Invest 87

      Pages: 49-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] CXCL12-CXCR4 engagement is required for migration ofcutaneous dendritic cells.2007

    • Author(s)
      Kabashima K
    • Journal Title

      Am J Pathol 171

      Pages: 1249-57

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] CXCR4 engagement promotes dendritic cell survival andmaturation.2007

    • Author(s)
      Kabashima K
    • Journal Title

      Biochem Biophys Res Commun 361

      Pages: 1012-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Inhibition of T helper 2 chemokine production bynarrowband ultraviolet B in cultured keratinocytes.2007

    • Author(s)
      Hino R
    • Journal Title

      Br J Dermatol 156

      Pages: 830-837

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Stimulation of Langerhans cells with ketoprofen plusUVA in murine photocontact dermatitis to ketoprofen.2007

    • Author(s)
      Atarashi K
    • Journal Title

      J Dermatol Sci 47

      Pages: 151-159

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] T cell populations propagating in the peripheral blood of patients with drug eruptions.2007

    • Author(s)
      Nishio D
    • Journal Title

      J Dermatol Sci 48

      Pages: 25-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Induction of kerationcyte apoptosis by photosensitizin chemicals plus UVA2007

    • Author(s)
      Kurita M
    • Journal Title

      J Dermatol Sci 45

      Pages: 105-112

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of T helper 2 chemokine production by narroebandultraviolet B in cultured kerationcytes2007

    • Author(s)
      Hino R
    • Journal Title

      Br J Dermatol 156

      Pages: 830-837

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] T cell populations propagating in the peripheral blood of patients with drug eruptions2007

    • Author(s)
      Nishio D
    • Journal Title

      J Dermatol Sci 48

      Pages: 25-33

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Who is really in control of skin immunity under physiological circumstances-lymphocytes,dendritic cells or keratinocytes2006

    • Author(s)
      Schroder JM
    • Journal Title

      Exp Dermatol 15

      Pages: 913-916

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Viewpoint 22006

    • Author(s)
      Kabashima K
    • Journal Title

      Exp Dermatol 15

      Pages: 919-920

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Establishment of murine model of allergic photocontact dermatitis to ketoprofen and characterization of pathogenic T cells2006

    • Author(s)
      Imai S
    • Journal Title

      J Dermatol Sci 41

      Pages: 127-136

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Solar urticaria as a manifestation of Churg-Strauss syndrome2006

    • Author(s)
      Shimauchi T
    • Journal Title

      Clin Exp Dermatol 32

      Pages: 209-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Innate immunity mediated by epidermal keratinocytes promotes acquired immunity involving Langerhans cells and T cells in the skin2006

    • Author(s)
      Sugita K
    • Journal Title

      Clin Exp Immunol 147

      Pages: 176-183

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 薬剤性光線過敏症2006

    • Author(s)
      戸倉 新樹
    • Journal Title

      臨床と研究 83

      Pages: 87-90

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 光アレルギーの発症機序と対策2006

    • Author(s)
      戸倉 新樹
    • Journal Title

      アレルギー 55

      Pages: 1382-1389

    • NAID

      10019986332

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Who is really in control of skin immunity underphysiological circumstances - lymphocytes, dendritic cellsor keratinocytes?2006

    • Author(s)
      Schroder JM
    • Journal Title

      Exp Dermatol 15

      Pages: 913-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Viewpoint 2.2006

    • Author(s)
      Kabashima K
    • Journal Title

      Exp Dermatol 15

      Pages: 919-920

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Establishment of murine model of allergicphotocontact dermatitis to ketoprofen andcharacterization of pathogenic T cells.2006

    • Author(s)
      Imai S
    • Journal Title

      J Dermatol Sci 41

      Pages: 127-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Solar urticaria as a manifestation of Churg-Strausssyndrome.2006

    • Author(s)
      Shimauchi T
    • Journal Title

      Clin Exp Dermatol 32

      Pages: 209-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Innate immunity mediated by epidermal keratinocytespromotes acquired immunity involving Langerhans cellsand T cells in the skin.2006

    • Author(s)
      Sugita K
    • Journal Title

      Clin Exp Immunol 147

      Pages: 176-183

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Who is really in control of skin immunity under physiological circumstances - lymphocytes, dendritic cells or keratinocytes?2006

    • Author(s)
      Schroder JM, Reich K, Kabashima K, Liu FT, Romani N, Metz M, Kerstan A, Lee PH, Loser K, Schon MP, Maurer M, Stoitzner P, Beissert S, Tokura Y, Gallo RL, Reich K
    • Journal Title

      Exp Dermatol 15

      Pages: 913-916

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Viewpoint 22006

    • Author(s)
      Kabashima K, Tokura Y
    • Journal Title

      Exp Dermatol 15

      Pages: 919-920

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Establishment of murine model of allergic photocontact dermatitis to ketoprofen and characterization of pathogenic T cells.2006

    • Author(s)
      Imai S, Atarashi K, Ikesue K, Akiyama K, Tokura Y
    • Journal Title

      J Dermatol Sci 41

      Pages: 127-136

    • NAID

      10020550425

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Solar urticaria as a manifestation of Churg-Strauss syndrome.2006

    • Author(s)
      Shimauchi T, Kabashima K, Tokura Y
    • Journal Title

      Clin Exp Dermatol

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Innate immunity mediated by epidermal keratinocytes promotes acquired immunity involving Langerhans cells and T cells in the skin.2006

    • Author(s)
      Sugita K, Kabashima K, Atarashi K, Shimauchi T, Kobayashi M, Tokura Y
    • Journal Title

      Clin Exp Immunol 147

      Pages: 176-183

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 薬剤性光線過敏症2006

    • Author(s)
      戸倉新樹
    • Journal Title

      臨床と研究 83

      Pages: 87-90

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 光アレルギーの発症機序と対策2006

    • Author(s)
      戸倉新樹
    • Journal Title

      アレルギー 55

      Pages: 1382-1389

    • NAID

      10019986332

    • Related Report
      2006 Annual Research Report
  • [Presentation] Spectrum of drug photoallergy2007

    • Author(s)
      Tokura Y
    • Organizer
      The 15th Korea-Japan Joint Meeting of Dermatology
    • Place of Presentation
      Jeju,Korea
    • Year and Date
      2007-06-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 光と薬剤アレルギー2007

    • Author(s)
      戸倉 新樹
    • Organizer
      2006年度日本皮膚科学会東京支部企画前実績研修講習会
    • Place of Presentation
      東京
    • Year and Date
      2007-02-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Narrowband UVBおよびPUVA療法の細胞生物学的効果2007

    • Author(s)
      戸倉 新樹
    • Organizer
      アトピー性皮膚炎治療研究会
    • Place of Presentation
      大阪
    • Year and Date
      2007-01-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Spectrum of drug photoallergy.2007

    • Author(s)
      Tokura Y
    • Organizer
      The 15th Korea-Japan Joint Meeting of Dermatology Jeju
    • Place of Presentation
      Korea
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 光線過敏症の診断と治療2006

    • Author(s)
      戸倉 新樹
    • Organizer
      第57回日本皮膚科学会中部支部学術大会
    • Place of Presentation
      名古屋
    • Year and Date
      2006-10-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 小児光線過敏症の診断とスキンケア2006

    • Author(s)
      戸倉 新樹
    • Organizer
      第30回日本小児皮膚科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2006-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Eosinophilic drug photoallergy induced by afloqualone and UVA in mice2006

    • Author(s)
      Nishio D
    • Organizer
      67th Annual Meeting of the Society for Investigative Dermatology
    • Place of Presentation
      Philadelphia,USA
    • Year and Date
      2006-05-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Eosinophilic drug photoallergy induced by afloqualone and UVA in mice.2006

    • Author(s)
      Nishio D
    • Organizer
      67th Annual Meetingof the Society for Investigative Dermatology Philadelphia
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Induction of eosinophil-infiltrating drug photoallergy in mice.2006

    • Author(s)
      Nishio D
    • Organizer
      International Symposium of Adverse Drug Reaction(ADR)in Yokohama
    • Place of Presentation
      Yokohama, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 目で見るアレルギー司性皮膚疾患2007

    • Author(s)
      戸倉 新樹
    • Total Pages
      5
    • Publisher
      南山堂,片山一朗,古川福実編集
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Environmental Dermatology環境, 職業からみた皮膚疾患2007

    • Author(s)
      戸倉 新樹
    • Total Pages
      6
    • Publisher
      文光堂,戸倉新樹他編
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 目で見るアレルギー性皮膚疾患2007

    • Author(s)
      戸倉 新樹
    • Total Pages
      5
    • Publisher
      南山堂、片山一朗,古川福実編集
    • Related Report
      2007 Annual Research Report
  • [Book] Environmental Dermatology 環境・職業からみた皮膚疾患2007

    • Author(s)
      戸倉 新樹
    • Total Pages
      6
    • Publisher
      文光堂、戸倉新樹 他 編
    • Related Report
      2007 Annual Research Report
  • [Book] 皮膚疾患の最新医療2006

    • Author(s)
      戸倉 新樹
    • Total Pages
      4
    • Publisher
      先端医療技術研究所
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 皮膚科診療プラクティス.19.薬疹を極める2006

    • Author(s)
      戸倉 新樹
    • Total Pages
      3
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 皮膚疾患の最新医療2006

    • Author(s)
      戸倉新樹
    • Total Pages
      318
    • Publisher
      先端医療技術研究所
    • Related Report
      2006 Annual Research Report
  • [Book] 皮膚科診療プラクティス.19.薬疹を極める2006

    • Author(s)
      戸倉新樹
    • Total Pages
      301
    • Publisher
      文光堂
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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