Redox state and biomolecular mechanism underlying the oxidative vulnerability of neonatal isolation model
| Project/Area Number |
18591297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | University of Miyazaki |
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Principal Investigator |
UEDA Yuto University of Miyazaki, Faculty of Medicine, Associate Professor (70244192)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Akira University of Miyazaki, Faculty of Medicine, Associate Professor (10041857)
DOI Taku University of Miyazaki, Faculty of Medicine, Research Associate (70274793)
ISHIDA Yasushi University of Miyazaki, Faculty of Medicine, Professor (20212897)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,780,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Glutamate / Redox / GABA / Neonatal isolation / kindling |
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| Research Abstract |
Nitroxide radical has been reported to react with hydroxyl radical and superoxide anion radical in the presence of cysteine or NADH. In this report the EPR SI of nitroxide radicals did not decay exponentially in the reaction with other radicals. The signal decay in this reaction is in accord with a linear function. On the other hand, its EPR SI decayed exponentially in the reaction with the reductants. Therefore, the reaction dynamics of nitroxide radicals with the reductants differed from that with other radicals. In the present study, the EPR SI of hydroxymethyl-PROXYL decayed exponentially. Therefore, the shortening of the halflife in the NI group without acute stress does not arise due to the reaction of nitroxide radical to other radicals (such as superoxide radical and hydroxyl radical). This indicates the enhancement of reducing ability. Therefore, the results obtained in this study indicate that while the intracerebral reducing ability was significantly depleted by acute stress in the NI group, this depletion did not occur in the control rats. It was also found that the intracerebral reducing ability significantly increased in the rats that had been subjected to NI when the acute stress was not applied. It is thought that the in vivo reducing ability is decreased due to mitochondrial damage, and a previous study reported that immobilization stress induced mitochondrial dysfunction. Therefore, the depletion of the intracerebral reducing ability after immobilization in the NI group may be related to mitochondrial damage in the brain. However, interpretation of the mechanisms behind the enhancement of intracerebral reducing ability in the NI group without the acute stress is now under way. We believe that impairment of the intracerebral reducing ability caused by acute stress in the NI rats is important biological evidence of cerebral vulnerability to acute stress in this model.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] Age-dependent changes in the hippocampal antioxidant ability of EL mice.2007
Author(s)
Takaki, M., Ueda, Y., Doi, T., Nagatomo, K., Murashima, YL, Nakajima, A., Kannan, H
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Journal Title
Neurosci Res 58(3)
Pages: 336-8
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Effect of levetiracetam on molecular regulation of hippocampal glutamate and GABA transporters in rats with chronic seizures induced by amygdalar FeCl(3) injection.2007
Author(s)
Ueda, Y., Doi, T., Nagatomo, K., Tokumaru, J., Takaki, M., Willmore, LJ
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Journal Title
Brain Res 1151
Pages: 55-61
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Generation of lipid radicals in the hippocampus of neonatal rats after acute hypoxic-ischemic brain damage2006
Author(s)
Ueda, Y., Noor, JI, Nagatomo, K., Doi, T., Ikeda, T., Nakajima, A., Ikenoue, T
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Journal Title
Exp Brain Res 169(1)
Pages: 117-121
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Molecular regulation of anti-oxidant ability in the hippocampus of EL mice.2007
Author(s)
M., Takaki, Y., Ueda, T., Doi, K., Nagatoio, Y.L., Murashima, L.J., illmore, H., Kannnan
Organizer
American Epilepsy Society
Place of Presentation
Philadelphia
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Neuroprotective effect of levetiracetam is derived from the synergistic effect on endogenous anti-oxidant in the hippocampus of rats.2007
Author(s)
Ueda, Y., Takaki, M., Doi, T., Nagatomo, K., Nakajima, A., Willmore, J
Organizer
American Epilepsy Society
Place of Presentation
Philadelphia
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] The functional role of glutamate transporter associated protein(GTRAP3-18) in the epileptogenesis induced by PTZ-kindling2006
Author(s)
Ueda, Y., Doi, T., Nagatomo, K., Takaki, M., Nakajima, A., Willmore, LJ
Organizer
1st North American Regional Epilepsy Congress, 60th Annual Meetings of the American Epilepsy Society &Canadian League Against Epilepsy
Place of Presentation
San Diego, California
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Measurement of antioxidant status using EPR and microdialysis during seizures in the EL mouse.2006
Author(s)
Takaki, M., Ueda, Y., Nakajima, A., Doi, T., Nagatomo, K., Willmore, LJ
Organizer
1st North American Regional Epilepsy Congress, 60th Annual Meetings of the American Epilepsy Society &Canadian League Against Epilepsy
Place of Presentation
San Diego, California
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] In vivo EPR Estimation of Bilateral Hippocampal Antioxidant Ability of Rats with Epileptogenesis induced by Amygdalar FeCl3 Microinjection2006
Author(s)
Willmore, LJ., Ueda, Y., Yokoyama, H., Nakajima, A., Takaki, M., Nagatomo, K., Doi, T
Organizer
1st North American Regional Epilepsy Congress, 60th Annual Meetings of the American Epilepsy Society &Canadian League Against Epilepsy
Place of Presentation
San Diego, California
Description
「研究成果報告書概要(欧文)」より
Related Report
