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Free radical production from betaAmyloid oligomers and mechanism of neurotoxicity deduced from them

Research Project

Project/Area Number 18591298
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionUniversity of Miyazaki

Principal Investigator

HAYASHI Yoshihito  University of Miyazaki, Faculty of Medicine, Assistant Professor (20295223)

Co-Investigator(Kenkyū-buntansha) ISHIDA Yasushi  UNIVERSITY OF MIYAZAKI, Faculty of Medicine, Professor (20212897)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,490,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsbeta amyloid / free radical / oligomer / βアミロイド / フリーラジカル / 凝集 / 神経毒性
Research Abstract

It is well known that beta amyloid (Aβ) peptides aggregate and make senile plaques up in AD patients' brain. So far the causes of AD is thought to be due to the excessive production of Aβ and its toxicity against neuronal cells in the brain in the process of aggregation. One of the main toxicities that Aβ shows against neuronal cells is due to productions of free radicals. It is thought that the stage when free radicals are produced maximally by Aβ is the very early period and it is just when Aβ changes into oligomers such as dimmer, timer and tetramer. This time we make oligomers (dimmer, trimmer, tetramer) using photocross-linking methods and separate them individually by using size gelchoromatography. Then we measured how much these oligomers produce H2O2 using APR solution and how many % of cells survive in the circumstances with Aβ oligomers. The results were that the molecular size of oligomers goes up there is tendency that the production of H2O2 is going up within 24 hours but there are no apparent differences between four forms of Aβ oligomers. The survive rate of the cells after 48hours about 60% cell were survived and there were also no apparent differences between Aβ oligomers. Therefore it will be needed that we should do further experiment using more bigger size oligomers such as tetramer, hexamer and soon carefully.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 7 results) Presentation (2 results)

  • [Journal Article] 抗うつ薬とperospironeによる増強療法が奏功した薬物治療抵抗性うつ病の2症例2007

    • Author(s)
      長友 慶子, 石塚 雄太, 林 要人, 他
    • Journal Title

      臨床精神医学 36

      Pages: 1293-1298

    • NAID

      40015679883

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 宮崎大学医学部附属病院精神科におけるコンサルテーション・リエゾン精神医療の現状2007

    • Author(s)
      長友 慶子, 並木 薫, 林 要人, 他
    • Journal Title

      宮崎県医師会医学会誌 312

      Pages: 15-20

    • NAID

      120007123010

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ベンゾジアゼピン系薬物依存症にSSRIが有効であった1例2007

    • Author(s)
      船橋 英樹, 林 要人, 他
    • Journal Title

      九州神経精神医学 53

      Pages: 74-74

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] ベンゾジアゼピン系薬物依存症にSSRIが有効であった1例2007

    • Author(s)
      船橋英樹, 林要人, 他
    • Journal Title

      九州神経精神医学 53

      Pages: 74-74

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 抗うつ薬とperospironeによる増強療法が奏功した薬物治療抵抗性うつ病の2症例2007

    • Author(s)
      長友慶子, 石塚雄太, 林要人, 他
    • Journal Title

      臨床精神医学 36

      Pages: 1293-1298

    • NAID

      40015679883

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 宮崎大学医学部附属病院精神科におけるコンサルテーション・リエゾン精神医療の現状2007

    • Author(s)
      長友慶子, 並木薫, 林要人, 他
    • Journal Title

      宮崎県医師会医学会誌 312

      Pages: 15-20

    • NAID

      120007123010

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma amyloid-beta concentrations in Alzheimer's disease:an alternative hypothesis2006

    • Author(s)
      Fullwood NJ, Hayashi Y, Allsop D.
    • Journal Title

      Lancet Neurol 12

      Pages: 1000-1001

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Plasma amyloid-beta concentrations in Alzheimer's disease : an alternative hypothesis2006

    • Author(s)
      Fullwood, NJ., Havashi, Y., Allsop, D
    • Journal Title

      Lancet Neurol 5(12)

      Pages: 1000-1

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Plasma amyloid-beta concentrations in Alzheimer's disease : an alternative hypothesis.2006

    • Author(s)
      Fullwood NJ, Hayashi Y, Allsop D
    • Journal Title

      Lancet Neurol. 5・12

      Pages: 1000-1

    • Related Report
      2006 Annual Research Report
  • [Presentation] Clioquinol itself does not inhibit Hydrogenperoxide formation induced by amyloid-beta peptide2006

    • Author(s)
      Y. HAYASHI, Y. ISHIDA
    • Organizer
      国際精神薬理学会
    • Place of Presentation
      HIROSHIMA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Clioquinol itself does not inhibit Hydrogenperoxide formation induced by amyloid-beta peptide2006

    • Author(s)
      Yoshihito, Hayashi, Yasushi, Ishida
    • Organizer
      INTER NATIONAL PSYCHOPHARMACOLOGY
    • Place of Presentation
      HIROSHIMA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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