| Project/Area Number |
18591313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
OSADA Kenichi St.Marianna University School of Medicine, Department of Neuropsychiatry, Lecturer (20233504)
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| Co-Investigator(Kenkyū-buntansha) |
MATUI Hiroaki St. Marianna University School of Medicine, Radioisotope Research Institute, Professor (90181685)
YAMADA Mituhiko National Institute of Mental Helth, National Center of Neurology and Psychiatry, Director (60240040)
KIDA Satoshi Tokyo University of Agriculture, Faculty of Applied Bioscience, Department of Bioscience, Laboratory of Animal Molecular Biology, Associate Professor (80301547)
KANEKO Rituko Touyou University, Department of Bioscience, Professor (00161183)
KATOU Tomohiro St. Marianna University School of Medicine, Department of Biochemistry, Professor (80233807)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | milnacipran / SNRI / SSRI / NBD-F / binding protein |
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| Research Abstract |
Since both preclinical and clinical studies have clearly implicated the serotonin (5-HT) and norepine phrine neurotransmitter systems in the mechanism of action of antidepressants, a monoaminergic hypothesis of the pathophysiology of depression was put forward. Our study investigated how SSRI, SNRI acted in vivo neuron, and which protein bond antidepressants.
We used milnacipran (SNRI) hiaffinity colum and applied rat brain cytosol fraction. Antidepressants binding protein was detected the 2D analysis system variable imageanalyzer, and identify by the mass spectrometry.
Milnacipran bond 4-Fluoro-7-nitro-2, 1, 3- benzoxadiazole (NBD-F) was detected in vivo PC12 cells by confocal microscope. We investigated that milnacipran bond NBD-F colocalize antidepressants binding protein by confocal microscope.
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