Research Project
Grant-in-Aid for Scientific Research (C)
Heptocellular carcinoma (HCC) often shows multi-step hepatocarcinogenesis from dysplastic nodule (DN) to overt hypervascular HCC. Therefore, it has been considered that administration of antiangiogenic factors during early stage of hepatocarcinogenesis might prevent or delay the development of HCC. For this purpose, basic and clinical studies were carried out. To investigate the morphological change of intratumoral microvessels after administration of thalidomide (antiangiogenic agent) in occult hepatic metastases experimentally induced in rats were analyzed by fluorescent vital microscopy and immunohistochemistry. Thalidomide exerted antiangiogenic effect on the occult hepatic metastases, however, the different vascular components in the tumor vasculature demonstrated various responses to antiangiogenic therapy. The vascular changes during hepatocarcinogenesis were examined with respect to vascular endothelial growth factor (VEGF) and its receptors (Flt-1 and Flk-1), hypoxia inducible factor-1 (HIF-1), and CD34 in DNs and HCCs. The capillarized areas with increased numbers of unpaired arteries in DNs were considered to represent an early malignant transformation. Increased expression of Flk-1 and HIF-1 associated with VEGF was involved in sinusoidal capillarization and the increased numbers of unpaired arteries in these areas. To evaluate the effectiveness of gelatine hydrogel as a carrier of antiangiogenic factor for transcatheter arterial embolization therapy, bFGF (strong. angiogenic factor) contained gelatine hydrogel was directly injected into the rat liver. However, no definite effect was confirmed, and further technical improvement is necessary for this purpose. The early detection of hypervascular foci in hypovascular DNs by imaging is important to know the time of antiangiogenic therapy, and dynamic CT and/or MRI were considered to be effective in clinical practice.
All 2008 2007 2006 Other
All Journal Article (49 results) (of which Peer Reviewed: 27 results) Presentation (26 results) Book (1 results) Remarks (2 results)
Abdom Imaging 33
Pages: 80-86
Abdom Imaging. (掲載確定)
Eur Radiol. (掲載確定)
Eur Radiol. Apr 2 (Epub ahead of print)
Hum Pathol. 38
Pages: 1532-1546
Histopathology 51
Pages: 390-400
Radiology 243
Pages: 386-395
Abdom Imaging 32
Pages: 470-474
Clin Exp Immunol. 147
Pages: 296-305
J Vasc Interv Radiol. 18
Pages: 365-376
Dig Dis Sci. 52
Pages: 1987-1994
Eur Radiol 17
Pages: 2969-2982
Pages: 1111-1117
Hepatol Res. 37
10019773136
J Comput Assist Tomogr. 31
Pages: 557-563
Phys Med Biol. 52
Pages: 6461-6474
Hum Pathol 38
Clin Exp Immunol 147
J Vasc Intery Radiol 18
Dig Dis Sci 52
Hepatol Res 37
Pages: 5200-205
Comput Assist Tomogr 31
Phys Med Biol 52
Abdom Imaging. Jul 4 (Epub ahead of print)
120001135713
Abdom Imaging. Jul 27 (Epub ahead of print)
Phys Med Biol. 7
J Vasc Interv Radiol. 18(3)
Rediology. 2007 Mar 13;[Epub ahead of print] (印刷中)
Abdo Imaging 2007 Mar 3;[Epub ahead of print] (印刷中)
Ckin Exp Immunol. 147(2)
Abdom Imaging,2006Dec 7;[Epub ahead of print] (印刷中)
187(1)
http://web.kanazawa-u.ac.jp/%7Emed22/
