Development of molecular imaging of brain function by using multi-nuclei magnetic resonance method and its application to analysis of cerebral disorders

• Project/Area Number: 18591362
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: NARUSE Shoji Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Associate Professor (50106407)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Tsunehiko Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Professor (70237733) ; TANAKA Chuzo Meiji University of Integrative Medicine, Medical MR Center, Professor (80163541)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: Magnetic Resonance Imaging / Molecular Imaging / Brain Function / 2D MR spectroscopy / Diffusion Tensor Imaging / Mn-enhanced MRI / Neuronal Regeneration / Inmmunology / 脳機能分子イメージング / Mn感受性物質 / MR感受性物質

Research Abstract

We have developed multi-nuclear magnetic resonance imaging (MRI) method for analysis of neuronal function and pathological conditions. Those are; (1) Neuronal activation imaging by using manganese-enhanced MRI (MEMRI): This method depends on the theory that Mn ion can enter into neuronal cell in conjunction with calcium ion during the neuronal activation; (2) Magnetic resonance technique which can visualize antigen-antibody reaction in the brain: First, we composed the macromolecule which is combined with magnetic resonance sensitive materials (Fe, Mn). Then, we developed the imaging of those material in vitro and in vivo systems. (3) Also we applied this method to the basic study for imaging of the specific reaction between tumor-cell and anti-tumor lymphocyte. (4) Two dimensional MR spectroscopy by which various brain metabolites can be analyzed precisely. This is developed by using ultra-fast MRI technique. (4) Improvement of conventional MR techniques such as; (1) Fiber tracking im aging method by using diffusion tensor images; (2) Perfusion imaging by using arterial spin tagging method, (3) Improvement of sensitivity of fMRI.
In MEMRI method, neuronal activation of the sensory area is clearly demonstrated by the paw stimulation in rat. Fibers were clearly demonstrated in rat olfactory grove, optic nerve and radiation, and the six cortical layers were clearly demonstrated by this method. The macromolecule combined Mn ion is clearly demonstrated on MR images, and also there is a possibility of demonstrating lymphocyte containing Fe ion. However it requires a relatively large amount of ion. In fiber tracking imaging method, disconnection of fiber was demonstrated in cases of cerebral infarction. In 2D-MRS, 2D-JPRESS and 2D-COSY were developed on 3.0T clinical MRI devices. Brain metabolites were detected sensitively by those 2D-MRS method. In conclusion, the multi-nulcear MR methods are useful to examine the brain function non-invasively. Since there are still more techniques in MRI methods to evaluate the brain function and metabolism, further research should be continued.

Research Products

• [Journal Article] Large - and Small - Scale Functional Organization of Visual Field Representation in the Human Visual Cortex (2008)
  • Author(s): Yamamoto, H., Ban, H., Fukunaga, M., Tanaka, C., Umeda, M., Ejima, Y
  • Journal Title: Portocello TA, Velloti RB (eds). Visual Cortex: New Research. New York: Nova Science Publisher (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Large-and Small-Scale Functional Organization of Visual Field Representation in the Human Visual Cortex. (2008)
  • Author(s): Yamamoto H, Ban H, Fukunaga M, Tanaka C, Umeda, Ejima Y
  • Journal Title: Visual Cortex: New Research, Nova Science Publisher, New York (in press)
  • Peer Reviewed
• [Journal Article] マンガン造影MRIによる分子イメージング (2007)
  • Author(s): 田中 忠蔵、青木 伊知男、河合 裕子、梅田 雅宏、樋口 敏宏
  • Journal Title: 映像情報 39 Pages: 366-371
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Molecular imaging by using Manganese -enhanced MRI (2007)
  • Author(s): Tanaka, C., Aoki, I., Kawai, Y., Umeda, M., Higuchi, T
  • Journal Title: Eizo-Jyoho 39(4)(in Japanese) Pages: 366-371
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Clinical application of MRI.-Diagnosis of brain function by fMRI(in Japanese) (2007)
  • Author(s): Fukunaga, M., Umeda, Higuchi, T., Tanaka, C
  • Journal Title: Nichidoku-Iho 52(3) Pages: 83-92
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] MRI as an anatomical imaging- Normal brain(in Japanese) (2007)
  • Author(s): Tanaka, C., Naruse, S., Higuchi, T., Umeda, M
  • Journal Title: In Ogawa S, Ueno S (eds): Handbook of Non-invasive Visualization Techniques. NTC, Inc, Tokyo Pages: 18-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] MR撮像法の臨床応用(6)-fMRIによる脳機能の診断 (2007)
  • Author(s): 福永雅喜、梅田雅宏、樋口敏宏、田中忠蔵
  • Journal Title: 日独医報 52 Pages: 83-92
  • Peer Reviewed
• [Journal Article] マンガン造影MRIによる分子イメージング (2007)
  • Author(s): 田中忠蔵、青木伊知男、河合裕子、梅田雅宏、樋口敏宏
  • Journal Title: 映像情報 39 Pages: 366-371
• [Journal Article] Toward a common circle:interhemispheric contextual modulati on in human early visual areas (2006)
  • Author(s): Ban H, Yamamoto H, Fukunaga M, Nakagoshi A, Umeda M, Tanaka C, Ejima Y
  • Journal Title: J.Neuroscience 26 Pages: 8804-8809
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 高齢予備世代の高血圧患者における脳MRI集団検診の意義 (2006)
  • Author(s): 金井 恵理、小川 史顕、中川 正法、西村 恒彦、松原 弘明、成瀬 昭二
  • Journal Title: CI研究 28 Pages: 35-41
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Toward a common circle: Interhemispheric contextual modulation in human early visual areas (2006)
  • Author(s): Ban, H., Yamamoto, H., Fukunaga, M., Nakagoshi, A., Umeda, M., Tanaka, C., Ejima, Y
  • Journal Title: J. Neuroscience 26(34) Pages: 8804-8809
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell labeling for magnetic resonance imaging with the T1 agent manganese chloride (2006)
  • Author(s): Aoki, I., Takahashi, Y., Chuang, K-H., Silva, AC., Igarashi, T., Tanaka, C., Childs, RW, Koretsky, AP
  • Journal Title: NMR in Biomedicine 19(1) Pages: 50-59
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Advantage and problem in ultra-high field MRI(in Japanese) (2006)
  • Author(s): Umeda, M., Fukunaga, M., Higuchi, T., Tanaka, C
  • Journal Title: Japanese Journal of Magnetic Resonance in Medicine 26(4) Pages: 189-199
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role of group brain checkups using magnetic resonance imaging in pre-elderly with hypertension(in Japanese) (2006)
  • Author(s): Kanai, E., Ogawa, F., Nakagawa, M., Nishimura, T., Matsubara, H., Naruse, S
  • Journal Title: Progress in Computed Imaging 28(1) Pages: 35-41
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 高齢予備世代の高血圧患者における脳MRI集団検診の意義 (2006)
  • Author(s): 金井恵理, 小川史顕, 中川正法, 西村恒彦, 松原弘明, 成瀬昭二
  • Journal Title: CI研究 28・1 Pages: 35-41
• [Journal Article] 超高磁場MRIの特徴と問題点 (2006)
  • Author(s): 梅田雅宏, 福永雅喜, 樋口敏宏, 田中忠蔵
  • Journal Title: 日本磁気共鳴医学会雑誌 26・4 Pages: 189-199
  • NAID: 10019266899
• [Journal Article] MRIによる分子イメージング (2006)
  • Author(s): 田中忠蔵, 青木伊知男, 河合裕子, 梅田雅宏, 樋口敏宏
  • Journal Title: 映像情報 Medical 10・9 Pages: 965-970
• [Journal Article] Toward a common circle : interhemispheric contextual modulation in human early visual areas (2006)
  • Author(s): Ban H, Yamamoto H, Fukunaga M, Nakagoshi A, Umeda M, Tanaka C, Ejima Y
  • Journal Title: The Journal of Neuroscience 26・34 Pages: 8804-8809
• [Presentation] High Time Resolution DWI of Muscle Contraction During Electrical Simulation (2007)
  • Author(s): Watanabe Y, Kimura K, Umeda M., Higuchi T, Tanaka C
  • Organizer: International Society for Magnetic Resonance in Medicine, 15th Scientific Meeting
  • Place of Presentation: Berlin,Germany
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional Imaging of Muscle Contraction caused by Electric Stimulation(Abstract 1726) (2007)
  • Author(s): Watanabe, Y., Kimura, K., Umeda, M., Aoki, I,. Higuchi, T., Tanaka, C., Naruse, S
  • Organizer: International Society for Magnetic Resonance in Medicine, 14th Scientific Meeting
  • Place of Presentation: Seattle, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Physiological Changes of Liver ADC values during Cardiac Cycle using DW-EPI(Abstract 1231) (2007)
  • Author(s): Umeda, M., Tanaka, C., Higuchi, T., Watanabe, Y., Kitamura, M., Aoki, I., Naruse, S
  • Organizer: International Society for Magnetic Resonance in Medicine, 14th Scientific Meeting
  • Place of Presentation: Seattle, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Improving contrast of iron oxide based cell labeling with manganese-enhanced MRI(Abstract 1815) (2007)
  • Author(s): Aoki, I., Kawai, Y., Jo, J-I., Tabata, Y., Umeda, M., Higuchi, T., Silva, AC., Tanaka ,C
  • Organizer: International Society for Magnetic Resonance in Medicine, 14th Scientific Meeting
  • Place of Presentation: Seattle, USA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Detection of Reactive Gliosis Using Manganese -Enhanced MRI (MEMRI)(Abstract 1215) (2007)
  • Author(s): Kawai, Y., Aoki, I., Matsumoto, N., Umeda, U., Higuchi, T., Kershaw, J., Silva, AC., Tanaka, C
  • Organizer: International Society for Magnetic Resonance in Medicine, 15th Scientific Meeting
  • Place of Presentation: Berlin, Germany
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] High Time Resolution DWI of Muscle Contraction During Electrical Simulation(Abstract 2635) (2007)
  • Author(s): Watanabe, Y., Kimura, K., Umeda, M., Higuchi, T., Tanaka, C
  • Organizer: International Society for Magnetic Resonance in Medicine, 15th Scientific Meeting
  • Place of Presentation: Berlin, Germany
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] High Time Resolution DWI of Muscle Contraction During Electrical Simulation (2007)
  • Author(s): Watanabe Y, Kimura K, Umeda M., Higuchi T, Tanaka C
  • Organizer: International Society for Magnetic Resonance in Medicine, 15th Scientific Meeting
  • Place of Presentation: Berlin, Germany
• [Book] 解剖画像としてのMRI-正常編。小川誠二、上野照剛(監修)非侵襲可視化技術ハンドブック 第1章MRIを用いる形態, 機能, 代謝の可視化 (2007)
  • Author(s): 田中 忠蔵、成瀬 昭二、樋口 敏宏、梅田 雅宏
  • Publisher: NTS,Inc.
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 解剖画像としてのMRI-正常編。小川誠二、上野照剛(監修)非侵襲可視化技術ハンドブック 第1章MRIを用いる形態, 機能, 代謝の可視化 (2007)
  • Author(s): 田中忠蔵、成瀬昭二、樋口敏宏、梅田雅宏
  • Total Pages: 10
  • Publisher: NTS, Inc.
• [Book] 「リハビリテーション医療」第23章:リハビリテーションで活用される検査法:「functional MRIの運動器疾患及びリハビリテーションヘの応用」(平澤泰介・田島文博(編)) (2007)
  • Author(s): 成瀬昭二, 平澤泰介, 勝見泰和, 田中忠蔵
  • Publisher: 金芳堂