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Does angiogenesis require smooth muscular cells?

Research Project

Project/Area Number 18591405
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionUniversity of Tsukuba

Principal Investigator

SAKAKIBARA Yuzuru (2007)  University of Tsukuba, Graduate School of Comprehensive Human Science, Professor (60192085)

佐藤 藤夫 (2006)  筑波大学, 大学院人間総合科学研究科, 講師 (20375497)

Co-Investigator(Kenkyū-buntansha) OHNEDA Osamu  University of Tsukuba, Graduate School of Comprehensive Human Science, Professor (30311872)
HYODO Kazuyuki  Photon Factory, High EnergyAcoelerator Research Organization, Assistant Professor (60201729)
榊原 謙  筑波大学, 大学院人間総合科学研究科, 教授 (60192085)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsRegenerative Medicine / Angiogenesis / Ischemic limb / Endothelial cell / Smooth muscular cell / Pericyte / Arteriogenesis / Mural Cell / 再生医学 / 放射光血管造影
Research Abstract

1. Investigation in relation to Endothelial Cell/Pericyte Adhesion and angiogenesis
Adhesive rate on capillary endothelial cell and pericyte was investigated in diabetic mice with hindlimb ischemia using Cilostazol, which is proved to be effective drug for peripheral vascular disease. Cilostazol-treated mice preserved both cell adhesion significantly compared with non-treated mice. At the same time, Cilostazol-treated mice showed higher rate of angiogenesis than non-treated mice significantly. This result implies that mural cells (pericytes and smooth muscle cells) are essential for angiogenesis. Moreover, their adhesion to endothelial cells is also important for angiogenesis.
2. Investigation on visualization of arteriogenesis using synchrotron radiation micro-angiography (SRMA)
Several types of endothelial progenitor cells (EPCs) were injected to ischemic hindlimb of nude mice. EPCs were classified according to Aldehyde Dehydrogenase activity. EPCs with low Aldehyde Dehydrogenase activity (Alde-low) showed higher vascular density (angiogenesis) than EPCs with high Aldehyde Dehydrogenase activity (Alde-high). At the same time, Alde-low showed higher number of arteriogenesis than Alde-high confirmed by SRMA. Since arteriogenesis was achieved by accumulation of smooth muscle cells in pre-existence arterioles, it is turned out that effective angiogenesis like application of Alde-low is essentially accompanied with smooth muscle cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (12 results)

All 2008 2007

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (3 results)

  • [Journal Article] Sex difference in peripheral arterial response to cold exposure.2008

    • Author(s)
      Sato F, Sakakibara Y, et. al.
    • Journal Title

      Circulation Journal 72(In press)

    • NAID

      110006835742

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Sex difference in peripheral arterial response to cold exposure2008

    • Author(s)
      Sato F, Sakakibara Y, et. al.
    • Journal Title

      Circulation Journal 72(in press)

    • NAID

      110006835742

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sex difference in peripheral arterial respones to cold exposure2008

    • Author(s)
      Sato F, Sakakibara Y, et. al.
    • Journal Title

      Circulation Journal 72(in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elucidation of Microvascular Response using Synchrotron Radiation Micro-Angiography.2007

    • Author(s)
      Matsushita S, Sato F, Sakakibara Y, et. al.
    • Journal Title

      Photon Factory Activity Report 2006 (Part A) Highlight and Faculty Report 24

      Pages: 51-52

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of erythropoietin on vascular regeneration of myocardial infarction assessed bysynchrotron radiation coronary angiography.2007

    • Author(s)
      Matsushita S, Sato F, Sakakibara Y, et. al.
    • Journal Title

      Photon Factory Activity Report 2006 (Part B) 24

      Pages: 264-264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Elucidation of Microvascular Response using Synchrotron Radiation Micro-Angiography2007

    • Author(s)
      Matsushita S, Sato F, Sakakibara Y, et. al.
    • Journal Title

      Photon Factory Activity Report 2006(Part A)Highlight and Faculty Report 24

      Pages: 51-52

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effect of erythropoietin on vascular regeneration of myocardial infarction assessed by synchrotron radiation coronary angiography2007

    • Author(s)
      Matsushita S, Sato F, Sakakibara Y, et. al.
    • Journal Title

      Photon Factory Activity Report 2006 24

      Pages: 264-264

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Elucidation of Microvascular Response using Synchrotron Radiation Micro-Angiography.2007

    • Author(s)
      Matsushita S, Sato F, Sakakibara Y, et. al.
    • Journal Title

      Photon Factory Activity Report 2006(Part A)Highlight and Faculty Report 24

      Pages: 51-52

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of erythropoietin on vascular regeneration of myocardial infarction assessed by synchrotron radiation coronary angiography.2007

    • Author(s)
      Matsushita S, Sato F, Sakakibara Y, et. al.
    • Journal Title

      Photon Factory Activity Report 2006(Part B) 24

      Pages: 264-264

    • Related Report
      2007 Annual Research Report
  • [Presentation] Sex difference in peripheral arterial response to cold exposure.2007

    • Author(s)
      Sato F, Sakakibara Y, et. al.
    • Organizer
      42nd Annual Meeting of Experimental Biology
    • Place of Presentation
      Washiington, D.C., U.S.A.
    • Year and Date
      2007-05-02
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sex difference in peripheral arterial response to cold exposure2007

    • Author(s)
      Sato F, Sakakibara Y, et. al.
    • Organizer
      42nd Annual Meeting of Experimental Biology
    • Place of Presentation
      Washington, D. C. U. S. A.
    • Year and Date
      2007-05-02
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Sex difference of arterial response to cold exposure2007

    • Author(s)
      Sato F, Sakakibara Y, et. al.
    • Organizer
      42nd Annual Meeting of Experimental Biology
    • Place of Presentation
      Washington, D. C., U.S.A.
    • Year and Date
      2007-05-02
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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