CANCER DIAGNOSIS BY SIMPLE SEQUENCE REPEAT IN THE INTRON THAT AFFECTS ALTERNATIVE APLICING OF C-MYCTRANSCRIPTIONALSUPPRESSOR,FIR
| Project/Area Number |
18591406
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
SAKATA Haruhito Chiba University, CHIBA UNIVERSITY HOSPITAL, FRONTIER SURGERY, ASSISTANT PROFESSOR (20375687)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUSHITA Kazuyuki CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICIE, EMERGENCYAND CRITICAL CARE MEDICINE, ASSISTANT PROFESSOR (90344994)
SHIMADA Hideaki CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICIE, FRONTIER SURGERY, ASSOCIATE PROFESSOR (20292691)
SUNAGA Masahiko CHIBA UNIVERSITY, CHIBA UNIVERSITY HOSPITAL, MOLECULAR DIAGNOSIS, ASSISTANT PROFESSOR (10361437)
TOMONAGA Takeshi CHIBA UNIVERSITY, GRADUATE SCHOOL OF MEDICIE, MOLECULAR DIAGNOSIS, ASSOCIATE PROFESSOR (80227644)
NISHIMORI Takanori CHIBA UNIVERSITY, CHIBA UNIVERSITY HOSPITAL, FRONTIER SURGERY, ASSISTANT PROFESSOR (30401003)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Alternative splicing / simple sequence repeat / intron / transcriptional factor / cancer diagnosis / c-mvc遺伝子 / c-myc / FIR / イントロン / スプライシングバリアント |
|---|
| Research Abstract |
Detection of cancer cells in the peripheral blood (PB) is pivotal for prediction of micro-metastasis, recurrence probability and/or outcome of the patients. Far UpStream Element (FUSE)-binding protein-interacting repressor, FIR, is a transcriptional repressor of c-myc gene. FIRΔexon2, exon 2-spliced variant of FIR, which lacks c-myc repression activity, was frequently discovered in human primary colorectal cancers but not in corresponding non-cancer epithelium, indicating its cancer-related expression. In this study, thus the expression level of FIRΔexon2 mRNA is examined in PB of 30 colorectal cancer patients and compared to those of 17 healthy volunteers by real-time polymerase chain reactions. As expected, FIRΔexon2 mRNA expression in PB of cancer patients significantly activated than those of healthy volunteers. Additionally, FIRΔexon2 as well as FIR mRNA is detected in PB even in the early stage (Dukes A stage) of colorectal cancer patients. The area under the receiving operating characteristic curve of FIRΔexon2 was greater than conventional carcinoembryonic antigen or carbohydrate antigen 19-9. Together, FIRΔexon2 mRNA in PB is a candidate of novel screening marker for colorectal cancer detection.
|
Report
(3 results)
Research Products
(32 results)
-
-
-
-
-
-
-
[Journal Article] Identificaton of novel immunohistochemical markers for primary hepatocellular carcinoma;clathrin heavy chain and formiminotransferase cyclodeaminase2008
Author(s)
Masanori S, Tomonaga T, Matsushita K, Sunaga M, Ohishi M, Kodera Y, Meda T, Takano S, Togawa A, Yoshitomi H, Otuka M, Yamamoto M, Nakano M, Miyazaki M, Nomura F.
-
Journal Title
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Journal Article] Primary Chemotherapy にTrastuzumabを併用した乳癌の2例2007
Author(s)
村上 健太郎, 坂田 治人, 宮澤 幸正, 松下 一之, 阿久 津泰典, 西森 孝典, 米山 泰生, 碓井 彰大, 加野 将之, 松原 久裕, 落合 武徳
-
Journal Title
癌と化学療法 34-10
Pages: 1683-1687
Related Report
Peer Reviewed
-
-
[Journal Article] Gene expression profiling induced by histone deacetylase inhibitor,FK228,in human esophageal squamous cancer cells2007
Author(s)
Hoshino I, Matsubara H, Akutsu Y, Nishimori T, Yoneyama Y, Murakami K, Komatsu A, Sakata H, Matsushita K, Ochiai T
-
Journal Title
Oncol Rep. 18(3)
Pages: 585-92
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Presentation] 食道癌ESD後の追加治療-sm,ly(+)にて手術を施行した1例.2007
Author(s)
坂田 治人, 阿久津 泰典, 松原 久裕, 岡住 慎一, 島田 英昭, 西川 貴雄, 首藤 潔彦, 白鳥 享, 赤井 崇, 落合 武徳
Organizer
62回日本消化器外科学会総会
Place of Presentation
東京
Year and Date
2007-07-19
Related Report
-
-
-
-
-
-
-
-
