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Analysis of the antitumor immunity activity of α-GalCer according to each organ against the human tumor cell line

Research Project

Project/Area Number 18591443
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

FUJI Nobuaki  Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (90332949)

Co-Investigator(Kenkyū-buntansha) ITOH Tsuyoshi  Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Assistant Professor (40420707)
YAMAGISHI Hisakazu  Kyoto Prefectural University of Medicine, President (40128723)
上田 祐二  京都府立医科大学, 医学研究科, 講師 (60254356)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsα-GalCer / α-GalCer
Research Abstract

We presented mechanism of the antitumor effect of a-GalCer using a murine spontaneous hepatic metastases model. Therefore this purpose was to investigate antitumor effect of a-GalCer against the human tumor cell line in vitro. At first lymphocytes were divided from human peripheral blood or liver tissue, and a-GalCer was added in them in vitro. Cellular cytotoxicity for the human tumor cell line and a production of cytokine having the anti-tumor activity were measured, and effectors cells were analyzed subsequently. Furthermore, we schemed to investigate whether anti-tumor activity of a-GalCer had organ specificity in a human. The reason was because a-GalCer administration could become a treatment for the metastasis lesion of the cancer patient if this antitumor effect had organ specificity.
A study of the antitumor effect of peripheral blood lymphocyte and hepatic lymphocyte: From the patient who underwent abdominal operation peripheral blood and hepatic tissue were obtained, and peripheral blood lymphocytes and hepatic lymphocytes were divided. Human tumor cell line and a-GalCer were added at various kinds of concentration for each lymphocyte, and mixed culture was performed in vitro, and cytotoxic activity by the Cr release examination was measured. However, results having reproducibility could not be obtained in peripheral blood lymphocyte and hepatic lymphocyte. Therefore a further experiment was needed to establish optimal concentration of a-GalCer. Quantity of production of IFN -y or IL-12 could not be evaluated continuously.
Effectors analysis of the above lymphocytes: Effectors analysis was started in each lymphocyte for a few examples. However, we did not result in an outcome having reproducibility similarly. A process and an effort to prepare as possible the background of the patient will be most important in future. A scheme and an effort to keep as possible the background of the patient may be most important in future.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report

Research Products

(31 results)

All 2008 2007 2006

All Journal Article Presentation

  • [Journal Article] Port site herniation of the small bowel following laparoscopy-assisted distal gastrectomy:a case report2008

    • Author(s)
      Itoh T, Fuji N, Taniguchi H, Naito K(他3名;(2)番目)
    • Journal Title

      J Med Case Reports 2

      Pages: 48-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Port site herniation of the small bowel following laparoscopy-assisted distal gastrectomy: a case report.2008

    • Author(s)
      Itoh, T, Fuji, N, Taniguchi, H, Naito, K, et. al.
    • Journal Title

      J Med Case Reports 2, 48

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Port site herniation of the small bowel following laparoscopy-assisted distal gastrectomy:a case report.2008

    • Author(s)
      Itoh T, Fuji N, Taniguchi H, Naito K(他3名;(2)番目)
    • Journal Title

      J Med Case Reports 2

      Pages: 48-48

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful induction of clinically competent dendritic cells from granulocyte colony-stimulating factor-mobilized monocytes for cancer vaccine therapy2007

    • Author(s)
      Ueda Y, Itoh T, Fuji N, Yamagishi H(他10名;(3)番目)
    • Journal Title

      Cancer Immunol Immunother 56(3)

      Pages: 381-389

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Induction of Peptide-Specific Immune Response in Patients with Primary Malignant Melanoma of the Esophagus after Immunotherapy Using Dendritic Cells Pulsed with MAGE Peptides2007

    • Author(s)
      Ueda Y, Itoh T, Fuji N, Yamagishi H(他7名;(4)番目)
    • Journal Title

      Jpn J Clin Oncol 37(2)

      Pages: 140-145

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Successful induction of clinically competent dendritic cells from granulocyte colony-stimulating factor-mobilized monocytes for cancer vaccine therapy2007

    • Author(s)
      Uead, Y, Itoh, T, Fuji, N, Yamagishi, N, et. al.
    • Journal Title

      Cancer Immunol Immunother 56 (3)

      Pages: 381-389

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Induction of Peptide-Specific Immune Response in Patients with Primary Malignant Melanoma of the Esophagus after Immunotherapy Using Dendritic Cells Pulsed with MAGE Peptides.2007

    • Author(s)
      Ueda, Y, Itoh, T, Fuji, N, Yamagishi, H, et. al.
    • Journal Title

      Jpn J Clin Oncol 37 (2)

      Pages: 140-145

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Induction of Peptide-Specific Immune Response in Patients with Primary Malignant Melanoma of the Esophagus after Immunotherapy Using Dendritic Cells Pulsed with MAGE Peptides.2007

    • Author(s)
      Ueda Y, Itoh T, Fuji N, Yamagishi H(他7名;(4)番目)
    • Journal Title

      Jpn J Clin Oncol 37

      Pages: 140-145

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful induction of clinically competent dendritic cells from granulocyte colony-stimulating factor-mobilized monocytes for cancer vaccine therapy.2007

    • Author(s)
      Ueda Y, Itoh T, Fuji N, Yamagishi H(他10名;(3)番目)
    • Journal Title

      Jpn J Clin Oncol 56

      Pages: 381-389

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful induction of clinically competent dendritic cells from granulocyte colony-stimulating factor-mobilized monocytes for cancer vaccine therapy.2007

    • Author(s)
      Ueda Y, Itoh T, Fuji N, Yamagishi H (他10名;(3)番目)
    • Journal Title

      Cancer Immunol Immunother 56(3)

      Pages: 381-389

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Induction of Peptide-Specific Immune Response in Patients with Primaiy Malignant Melanoma of the Esophagus after Immunotherapy Using Dendritic Cells Pulsed with MAGE Peptides.2007

    • Author(s)
      Ueda Y, Itoh T, Fuji N, Yamagishi H (他7名;(4)番目)
    • Journal Title

      Jpn J Clin Oncol 37(2)

      Pages: 140-145

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Potassium oxonate,an enzyme inhibitor compounded in S-1,reduces the supPression of antiumor immunity induced by 5-fluorouracil2006

    • Author(s)
      Yamashita T, Ueda Y, Fuji N, Itoh T, Yamagishi H(他2名;(3)番目)
    • Journal Title

      Cancer Chemother Pharmacol 58(2)

      Pages: 183-188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mature dendritic cells generated from patient-derived peripheral blood monocytes in one-step culture using streptococcal prepazation OK-432 exert an enhanced antigen-presenting capacity2006

    • Author(s)
      Naito K, Ueda Y, Itoh T, Fuji N, Yamagishi H(他10名;(4)番目)
    • Journal Title

      Int J Oncol 28(6)

      Pages: 1481-1489

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A new strategy using autologous dendritic cells and lymphokine-activated cells for cancer immunotherapy:efficient maturation of DCs by co-culture with LAK cells in vitro2006

    • Author(s)
      Yano Y, Ueda Y, Itoh T, Fuji N, Yamagishi H(他8名;(4)番目)
    • Journal Title

      Oncol Rep 16(1)

      Pages: 147-152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Intraoperative pathological investigation of recurrent nerve nodal metastasis can guide the decision whether to perform cervical lymph node dissection in thoracic esophageal cancer2006

    • Author(s)
      Ueda Y, Fuji N, Itoh T, Yamagishi H(他8名;(8)番目)
    • Journal Title

      Oncol Rep 16(5)

      Pages: 1061-1066

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The range of tumor extension should have precedence over the location of the deepest tumor center in determining the regional lymph node grouping for widely extending esophageal carcinomas2006

    • Author(s)
      Ueda Y, Fuji N, Itoh T, Yamagishi H(他7名;(8)番目)
    • Journal Title

      Jpn J Clin Oncol 36(12)

      Pages: 775-782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Potassium oxonate, an enzyme inhibitor compounded in S-1, reduces the suppression of antitumor immunity induced by 5-fluorouracil.2006

    • Author(s)
      Yamashita, T, Ueda, Y, Fuji, N, Itoh, T, yamagishi, H, et. al.
    • Journal Title

      Cancer Chemother Pharmacol 58 (2)

      Pages: 183-188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mature dendritic cells generated from patient-derived peripheral blood monocytes in one-step culture using streptococcal preparation OK-432 exert an enhanced antigen-presenting capacity.2006

    • Author(s)
      Naito, K, Ueda, T, Fuji, N, Yamagishi, H, et. al.
    • Journal Title

      Int J Oncol 28 (6)

      Pages: 1481-1489

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A new strategy using autologous dendritic cells and lymphokine-activated killer cells for cancer immunotherapy: efficient maturation of DCs by co-culture with LAK cells in vitro.2006

    • Author(s)
      Yano, Y, Ueda, Y, Itoh, T, Fuji, N, Yamagishi, H, et. al.
    • Journal Title

      Oncol Rep 16 (1)

      Pages: 147-152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Intraoperative pathological investigation of recurrent nerve nodal metastasis can guide the decision whether to perform cervical lymph node dissection in thoracic esophageal cancer.2006

    • Author(s)
      Ueda, Y, Fuji, N, Itoh, Yamagishi, H, T, et. al.
    • Journal Title

      Oncol Rep 16 (5)

      Pages: 1061-1066

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The range of tumor extension should have precedence over the location of the deepest tumor center in determining the regional lymph node grouping for widely extending esophageal carcinomas.2006

    • Author(s)
      Ueda, Y, Fuji, N, Itoh, T, Yamagishi, H, et. al.
    • Journal Title

      Jpn J Clin Oncol 36 (12)

      Pages: 775-782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Potassium oxonate, an enzyme inhibitor compounded in S-1, reduces the suppression of antitumor immunity induced by 5-fluorouracil.2006

    • Author(s)
      Yamashita T, Ueda Y, Fuji N, Itoh T, Yamagishi H(他2名;(3)番目)
    • Journal Title

      Cancer Chemother Pharmacol 58(2)

      Pages: 183-188

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mature dendritic cells generated from patient-derived peripheral blood monocytes in one-step culture using streptococcal preparation OK-432 exert an enhanced antigen-presenting capacity.2006

    • Author(s)
      Naito K, Ueda Y, Itoh T, Fuji N, Yamagishi H(他10名;(4)番目)
    • Journal Title

      Int J Oncol 28(6)

      Pages: 1481-1489

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A new strategy using autologous dendritic cells and lymphokine-activated killer cells for cancer immunotherapy : efficient maturation of DCs by co-culture with LAK cells in vitro.2006

    • Author(s)
      Yano Y, Ueda Y, Itoh T, Fuji N, Yamagishi H(他8名;(4)番目)
    • Journal Title

      Oncol Rep 16(1Y)

      Pages: 147-152

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Intraoperative pathological investigation of recurrent nerve nodal metastasis can guide the decision whether to perform cervical lymph node dissection in thoracic esophageal cancer.2006

    • Author(s)
      Ueda Y, Fuji N, Itoh T, Yamagishi H (他8名;(8)番目)
    • Journal Title

      Oncol Rep 16(5)

      Pages: 1061-1066

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The range of tumor extension should have precedence over the location of the deepest tumor center in determining the regional lymph node grouping for widely extending esophageal carcinomas.2006

    • Author(s)
      Ueda Y, Fuji N, Itoh T, Yamagishi H (他7名;(8)番目)
    • Journal Title

      Jpn J Clin Oncol 36(12)

      Pages: 775-782

    • Related Report
      2006 Annual Research Report
  • [Presentation] Port site herniation of the small bowel following laparoscopy-assisted distal gastrectomy: a case report.2008

    • Author(s)
      Itoh, T, Fuji, N, Taniguchi, H, Naito, K, et. al.
    • Organizer
      J Med Case Reports
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Synchronous double cancer of the cystic duct and gallbladder associated with the low junction of the duct2007

    • Author(s)
      Itoh T, Fuji N, Taniguchi H, Watanabe T, Kosuga T, Kashimoto K, Naito K
    • Organizer
      1st biennial congress the Asian-Pacific Hepato-Pancreato-Biliary Association
    • Place of Presentation
      Fukuoka,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Outcome of supervised surgical training for the resection of colo-rectal cancer2007

    • Author(s)
      Itoh T, Fuji N, Taniguchi H, Amaike H, Watanabe T, Naito K
    • Organizer
      American Collegeof Surgeons 93rd annual clinical congress
    • Place of Presentation
      New Orleans,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Induction of Peptide-Specific Immune Response in Patients with Primary Malignant Melanoma of the Esophagus after Immunotherapy Using Dendritic Cells Pulsed with MAGE Peptides.2007

    • Author(s)
      Ueda, Y, Itoh, T, Fuji, N, Yamagishi, H, et. al.
    • Organizer
      Jpn J Clin Oncol
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Outcome of supervised surgical training for the resection of colo-rectal cancer.2007

    • Author(s)
      Itoh T, Fuji N, Taniguchi H, Naito K(他2名;(2)番目)
    • Organizer
      American College of Surgeons 93rd annual clinical congress
    • Place of Presentation
      New Orleans, USA
    • Related Report
      2007 Annual Research Report

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Published: 2006-03-31   Modified: 2016-04-21  

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