| Project/Area Number |
18591444
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
UCHIDA Eiji Nippon Medical School, Faculty of Medicine, Professor (70176684)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥1,250,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥150,000)
Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | pancreatic cancer / experimental model / hamster / imaging |
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| Research Abstract |
1. Characterization of PGHAM-1-Luc cells in vitro
We developed a new cells named PGHAM-1-Luc which is special PGHAM-1 cells with luciferase activity transduced by AAV vector. PGHAM-1-Luc-1 has most activity of luciferase among five subclones from PGHAM-1-Luc cells. Luciferase activity is measured by IVIS Imaging System (IVIS 100, Xenogen) with luciferin. Doubling time of PGHAM-1-Luc-1 was 18.2 hours that is longer than that of original PGHAM-1.
2. In vivo transplantation experiment using PGHAM-1-Luc-1 cells
Three models for PGHAM-1 ; 1)primary pancreatic cancer and simultaneous liver metastasis by intrapancreatic transplantation, 2)liver metastasis alone by intrasplenic transplantation, 3)peritoneal dissemination by intraperitoneal transplantation, within twenty-one days after transplantation.
Results of transplantation of PGHAM-1-Luc in hamsters.
Intrapancreatic transplantation model : Bioluminescence image was shown in primary and metastatic tumors at 14-21 days. The sin of bioluminescence was almost same as actual size at autopsy.
Intrasplenic transplantation model : Bioluminescence was found in spleen without induction of liver metastasis.
Intraperitoneal transplantation model : Very low number of disseminated nodules on peritoneum with faint bioluminescence.
From above data, the metastatic activity of PGHAM-1-Luc-1 was less than that of PGHAM-1.
3. Pathology of tumors
Histopathology of pancreatic tumor by PGHAM-1-Luc-1 transplantation is moderately of poorly differentiated adenocarcinoma. PGHAM-1 is well-differentiated one. Any changes would be induced through gene induction.
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