DOKI Yuichiro Osaka Univetsty, Graduate School of Medicine, Associate Professor (20291445)
MIYATA Hiroshi Osaka Univetsty, Graduate School of Medicine, Assistant Professor (80362713)
FUJIWARA Yoshiyuki Osaka Univetsty, Graduate School of Medicine, Assistant Professor (40314330)
KANTOU Tatsuya Osaka Univetsty, Greduate School of Medicine, Endowed Chair Associate Professor (80372613)
HATAZAWA Jun Osaka Univetsty, Graduate School of Medicine, Professor (70198745)
|Budget Amount *help
¥3,770,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
1 Basic study in human samples of esophageal cancers
In order to examine the relationship between tumor-infiltrating dendritic cells (DCs), lymphocyte, regulatory T cell and lymph node metastasis, we performed immunohistochemishy using antibodies including CD1a, 5-100, CD4, CD8, and Foxp3 in surgical specimens of esophageal cancers. We found that there was no significant correlation between the number of positive cells for those antibody and lymph node metastasis.
2 Setup for clinical study of neoadjuvant immunochemotherapy using dendritic cells for esophageal cancers
We generated matured DCs from PBMC in the following manner. Briefly, after leukapheresis collecting at least 5.0 x 10^8 mononuclear cells, monocytes isorated from PBMC were cultured in AIM-V media containing 50ng/ml GM-CSF and 20 ng/ml IL -4 for 48 hours. In addition, the cells were matured in conditioned-media with 0.1 KE/ml OK-432, 500IU/ml INFα, 50ng/ml PGE2 for final 24 hours. As a result, we confirmed that more than 3.6 x 10^6 matured DCs were generated, and that the viability of generated DCs was more than 70 %.
Next we labelled DCs with 〓In-Oxine, and labelled DCs was administer to a piece of meat (a substitute for tumors). Immediately and 24 hours after injection, we confirmed that administered DCs were detected by scintigraphic images.