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Proteomic profiling of autoantibodies in patients with esophageal squamous cell carcinoma

Research Project

Project/Area Number 18591465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionYamaguchi University

Principal Investigator

TAKEDA Shigeru  Yamaguchi University, Graduate School of Medicine, Assistant Professor (50403671)

Co-Investigator(Kenkyū-buntansha) OKA Masaaki  Yamaguchi University, Graduate School of Medicine, Professor (70144946)
TAKASHIMA Motonari  Yamaguchi University, Graduate School of Medicine, Assistant Professor (40420525)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsproteomics / Esophageal cancer / LC-MS / MS / Tropomyosin
Research Abstract

To identify proteins associated with esophageal carcinogenesis, we performed protein profiling of 16 esophageal squamous cell carcinomas (ESCCs) and paired non-cancerous tissues by two-dimensional electrophoresis (2-DE) and tandem mass spectrometry. In cancerous tissues, three spots showed significant up-regulation in the amount of protein, while eight spots were significantly down-regulated. The identities of the spots were determined by peptide mass fingerprinting (PMF) with liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) and were confirmed by immunoblotting. The up-regulated proteins were tropomyosin alpha 4 chain, transgelin, and pyruvate kinase. The down-regulated proteins were serum albumin precursor, isoforms of annexin Al, tropomyosin beta chain, 14-3-3 protein sigma and isoforms of serotransferrin precursor. In all 16 cases, up-regulation of the tropomyosin alpha 4 chain was confirmed by immunoblotting. Localization of the tropomyosin alpha 4 chain in ESCC cells and adjacent fibroblasts was confirmed by immunohistochemistry.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (13 results)

All 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (5 results)

  • [Journal Article] Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectromet.2007

    • Author(s)
      原田 俊夫
    • Journal Title

      Proteomics 1(2)

      Pages: 215-223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] A clinical study of esophagectomy after chemo-radiation therapy for advanced esophageal carcinoma2007

    • Author(s)
      武田 茂
    • Journal Title

      Gan To Kagaku Ryoho 34(12)

      Pages: 1982-1984

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry2007

    • Author(s)
      Toshio, Harada
    • Journal Title

      Prpteomics 1(2)

      Pages: 215-223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A clinical study of esophagectomy after chemo-radiation therapy for advanced esophageal carcinoma2007

    • Author(s)
      Shigeru, Takeda
    • Journal Title

      Gan To Kagaku Ryoho 34(12)

      Pages: 1982-1984

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of tropomyosin alpha 4 chain is increased in esophageal squamous cell carcinoma as evidenced by proteomic profiling by two-dimensional electrophoresis and liquid chromatography-mass spectrometry/mass spectrometry.2007

    • Author(s)
      原田 俊夫
    • Journal Title

      Proteomics 1(2)

      Pages: 215-223

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A clinical study of esophagectomy after chemo-radiation therapy for advanted esophageal carcinoma2007

    • Author(s)
      武田 茂
    • Journal Title

      Gan To Kagaku Ryoho 34(12)

      Pages: 1982-1984

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of autoantibodies in patients with hepatocellular carcinoma.2006

    • Author(s)
      高島元成
    • Journal Title

      PROTEOMICS 6(13)

      Pages: 3984-3900

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Increased expression and phosphorylation of liver glutamine synthetase in well-differentiated hepatocellular carcinoma tissues from patients infected with hepatitis C virus.2006

    • Author(s)
      蔵満保宏
    • Journal Title

      Electrophoresis 27(8)

      Pages: 1651-1658

    • Related Report
      2006 Annual Research Report
  • [Presentation] 3D CT-lymphographyを用いた食道癌におけるsentinel lymph nodeの検討2007

    • Author(s)
      武田 茂
    • Organizer
      第62回日本消化器外科学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2007-07-18
    • Related Report
      2007 Annual Research Report
  • [Presentation] 進行食道癌における術前化学放射線後食道切除症例の検討2007

    • Author(s)
      武田茂
    • Organizer
      第29回日本癌局所療法研究会
    • Place of Presentation
      ホテル日航東京
    • Year and Date
      2007-06-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 高齢者食道癌における食道切除術症例の検討2007

    • Author(s)
      武田 茂
    • Organizer
      第61回日本食道学会学術集会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2007-06-22
    • Related Report
      2007 Annual Research Report
  • [Presentation] 食道扁平上皮癌患者における血清蛋白自己抗体の解析2007

    • Author(s)
      原田俊夫
    • Organizer
      第107回日本外科学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Analysis for Auoantibodies in Sera from esophagus Squamous Cell Carcinoma-Bearing Patients2007

    • Author(s)
      Toshio, Harada
    • Organizer
      The 107th Annual Congress of Japan Surgical Society
    • Place of Presentation
      Osaka
    • Year and Date
      2007-04-12
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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