Study on stroma of scirrhous gastric carcinoma Down reguration of collagen synthesis by siRNA in tumor tissue

• Project/Area Number: 18591482
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kitasato University
• Principal Investigator: KIKUCHI Shiro Kitasato University, 医学部, 准教授 (30161417)
• Co-Investigator(Kenkyū-buntansha): ADACHI Eijiro 北里大学, 医療系・研究科, 教授 (30110430) ; FUTAWATARI Nobue 北里大学, 医学部, 助教 (40306600) ; NAGATA Kazuhiro 京都大学, 再生医科学研究所, 教授 (50127114) ; KUROYAMA Shinchi 北里大学, 医学部, 助教 (10286297)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,820,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: 胃癌 / スキルス / コラーゲン / HSP47 / 線維芽細胞 / 間質制御

Research Abstract

本研究では、スキルス胃癌組織の実験モデルを開発し、コラーゲン産生の抑制が新たな治療戦略となりうるかについて検討することを目的とした
標的分子トシテコラーゲン産生特異的分子シャペロン蛋白デアルheat shock protein 47(HSP47)ヲ選択シ、コノ標的蛋白質ニ対スル発現抑制ヲ行ウタメsmall interfering RNA (siRNA)導入癌細胞株ヲ作成シタ。スキルス胃癌モデルニオケル移植癌細胞ノ増殖状況ヲ、ウイルス導入前後デ比較シタ。結果、数例デウイルス導入株ニ癌細胞ノ増殖抑制ヲ認メタモノノ、大部分ノ移植片デハ増殖ニ明ラカナ違イハ認メラレズ、癌細胞自身カラ産生スルコラーゲン産生ノ関与ガアマリ大キクナイコトガ示サレタ

Research Products

• [Journal Article] Localization of the membrane-anchored MMP-regulator RECK at the neuromuscular junctions (2008)
  • Author(s): Kawashima S, Imamura Y, Ediriweera P. S. C, Noda T, Takahashi R, Adachi E, Takahashi C and Noda M
  • Journal Title: J Neurochem 104(2) Pages: 376-385
  • Peer Reviewed
• [Journal Article] Collagen-type specificity of glycoprotein VI as a determinant of platelet adhesion (2008)
  • Author(s): Jung Stephanie M, Takemura, Y, Imamura Y, Hayashi T, Adachi E, Moroi M
  • Journal Title: Platelets Feb 19(1) Pages: 32-42
  • Peer Reviewed
• [Journal Article] Intercellular accumulation of type V collagen fibrils in accordance with cell aggregation (2008)
  • Author(s): Kihara T, Imamura Y, Takemura Y, Mizuno K, Adachi E, and Hayashi T
  • Journal Title: J. Biochem 144(5) Pages: 625-633
  • NAID: 10024913455
  • Peer Reviewed
• [Journal Article] Keratinocyte Differentiation and proliferation are regulated by adhesion to the three-dimensional meshwork structure of type IV collagen (2008)
  • Author(s): Fujisaki H, Adachi E and Hattori Shunji
  • Journal Title: Connective Tissue Research 49(6) Pages: 426-436
  • Peer Reviewed
• [Journal Article] Versatile roles of R-Ras GAP in neurite formation of PC12 cells and embryonic vascular development (2007)
  • Author(s): Iwashita, S., Kobayashi, M., Kubo, Y., Hinohara, Y., Sezaki, M., Nakamura, K., Suzuki-Migishima, R., Yokoyama, M., Sato, S., Fukuda, M., Ohba, M., Kato, C., Adachi, E. and Song, S.Y
  • Journal Title: J. Biol. Chem 282(6) Pages: 3413-3417
  • Peer Reviewed
• [Journal Article] Keratinocyte apoptosis on type I collagen fibrils is prevented by Erk1/2 activation under high calcium conditions Connect (2007)
  • Author(s): Fujisaki H, Ebihara T, Irie S, Kobayashi T, Adachi E, Mochitate K, and Hattori S
  • Journal Title: Tissue Res. 48(3) Pages: 159-169
  • Peer Reviewed
• [Journal Article] Expression of an acyl-CoA synthetase, lipidosin, in astrocytes of the murine brain and its up-regulation during remyelination following cuprizone-induced demyelination (2007)
  • Author(s): Song Si-Young, Kato C, Adachi E, Moriya-Sato A, Inagawa-Ogashiwa M, Umeda R, Hashimoto N
  • Journal Title: J. Neurosci. Res. 85(16) Pages: 3586-3597
  • Peer Reviewed
• [Journal Article] Immunohistochemical Distribution of Heat Shock Protein 47 (HSP47) in Scirrhous Carcinoma of the Stomach (2006)
  • Author(s): Hirai K, Kikuchi S, et. al.
  • Journal Title: Anticancer Research 26 Pages: 71-78
  • Peer Reviewed
• [Journal Article] Elimination of epiplakin by gene targeting resuts in acceleration of keratinocyte migration in mice (2006)
  • Author(s): Goto Mizuki, Sumiyoshi Hideaki, Sakai Takao, Fossler R, Ohashi S, Adachi E, Yoshioka H, Fujiwara S
  • Journal Title: Molecular & Cellular Biology 26(2) Pages: 548-558
  • Peer Reviewed
• [Journal Article] 1 (2006)
  • Author(s): Hjrai K, Kikuchi S, et. al.
  • Journal Title: Anticancer Research 26 Pages: 71-78
  • Peer Reviewed
• [Journal Article] Immunohistochemical Distribution of Heat Shock Protein 47 (HSP47) in Scirrhous Carcinoma of the Stomach (2006)
  • Author(s): Hirai K, Kikuchi S, et al.
  • Journal Title: Anticancer Research 26 Pages: 71-78
• [Presentation] Restoration to a contractile phenotype from dedifferentiated status of smooth muscle cells in layered vascular constructs (2007)
  • Author(s): Eijiro Adachi, Takashi Aihara, Satoshi Hosoya and Hironobu Iwashiro
  • Organizer: Gordon Research Conference
  • Place of Presentation: アメリカ
• [Presentation] スキルス胃癌微小環境の再生と線維化制御の意義について (2006)
  • Author(s): 平井和弥、菊池史郎、栗田敦、安達栄治郎、渡邊昌彦
  • Organizer: 第78回日本胃癌学会
  • Place of Presentation: 横浜
• [Presentation] 細胞外マトリックスの生物学とバイオマテリアル足場 (2006)
  • Author(s): 安達栄治郎
  • Organizer: 第9回日本組織工学会
  • Place of Presentation: 京都(抄録集 p.80)
• [Book] 細胞外マトリックスの生物学 (2006)
  • Author(s): 安達栄治郎、相原孝至、大橋しほ花、浅野仁美、水野一乘
  • Publisher: ティッシュエンジニアリング, 日本医学館, 東京
• [Patent(Industrial Property Rights)] コラーゲン特異的分子シャペロンHSP47の測定方法 (2008)
  • Inventor(s): 渡邊昌彦, 菊池史郎他4名
  • Industrial Property Rights Holder: 学校法人北里学園
  • Patent Publication Number: 2008-122276
  • Acquisition Date: 2008-05-29
• [Patent(Industrial Property Rights)] 高密度培養組織の製造方法及び高密度培養組織 (2006)
  • Inventor(s): 安達栄治郎、大橋しほ花、他1名
  • Industrial Property Rights Holder: 学校法人北里学園
  • Acquisition Date: 2006-08-24
• [Patent(Industrial Property Rights)] コラーゲン特異的分子シャペロンHSP47の測定方法 (2006)
  • Inventor(s): 渡邊 昌彦 他
  • Industrial Property Rights Holder: 学校法人北里学園
  • Industrial Property Number: 2006-307831
  • Filing Date: 2006-11-14