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Study on stroma of scirrhous gastric carcinoma Down reguration of collagen synthesis by siRNA in tumor tissue

Research Project

Project/Area Number 18591482
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKitasato University

Principal Investigator

KIKUCHI Shiro  Kitasato University, 医学部, 准教授 (30161417)

Co-Investigator(Kenkyū-buntansha) ADACHI Eijiro  北里大学, 医療系・研究科, 教授 (30110430)
FUTAWATARI Nobue  北里大学, 医学部, 助教 (40306600)
NAGATA Kazuhiro  京都大学, 再生医科学研究所, 教授 (50127114)
KUROYAMA Shinchi  北里大学, 医学部, 助教 (10286297)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,820,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywords胃癌 / スキルス / コラーゲン / HSP47 / 線維芽細胞 / 間質制御
Research Abstract

本研究では、スキルス胃癌組織の実験モデルを開発し、コラーゲン産生の抑制が新たな治療戦略となりうるかについて検討することを目的とした
標的分子トシテコラーゲン産生特異的分子シャペロン蛋白デアルheat shock protein 47(HSP47)ヲ選択シ、コノ標的蛋白質ニ対スル発現抑制ヲ行ウタメsmall interfering RNA (siRNA)導入癌細胞株ヲ作成シタ。スキルス胃癌モデルニオケル移植癌細胞ノ増殖状況ヲ、ウイルス導入前後デ比較シタ。結果、数例デウイルス導入株ニ癌細胞ノ増殖抑制ヲ認メタモノノ、大部分ノ移植片デハ増殖ニ明ラカナ違イハ認メラレズ、癌細胞自身カラ産生スルコラーゲン産生ノ関与ガアマリ大キクナイコトガ示サレタ

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (18 results)

All 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (3 results) Book (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Localization of the membrane-anchored MMP-regulator RECK at the neuromuscular junctions2008

    • Author(s)
      Kawashima S, Imamura Y, Ediriweera P. S. C, Noda T, Takahashi R, Adachi E, Takahashi C and Noda M
    • Journal Title

      J Neurochem 104(2)

      Pages: 376-385

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Collagen-type specificity of glycoprotein VI as a determinant of platelet adhesion2008

    • Author(s)
      Jung Stephanie M, Takemura, Y, Imamura Y, Hayashi T, Adachi E, Moroi M
    • Journal Title

      Platelets Feb 19(1)

      Pages: 32-42

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intercellular accumulation of type V collagen fibrils in accordance with cell aggregation2008

    • Author(s)
      Kihara T, Imamura Y, Takemura Y, Mizuno K, Adachi E, and Hayashi T
    • Journal Title

      J. Biochem 144(5)

      Pages: 625-633

    • NAID

      10024913455

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Keratinocyte Differentiation and proliferation are regulated by adhesion to the three-dimensional meshwork structure of type IV collagen2008

    • Author(s)
      Fujisaki H, Adachi E and Hattori Shunji
    • Journal Title

      Connective Tissue Research 49(6)

      Pages: 426-436

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Versatile roles of R-Ras GAP in neurite formation of PC12 cells and embryonic vascular development2007

    • Author(s)
      Iwashita, S., Kobayashi, M., Kubo, Y., Hinohara, Y., Sezaki, M., Nakamura, K., Suzuki-Migishima, R., Yokoyama, M., Sato, S., Fukuda, M., Ohba, M., Kato, C., Adachi, E. and Song, S.Y
    • Journal Title

      J. Biol. Chem 282(6)

      Pages: 3413-3417

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Keratinocyte apoptosis on type I collagen fibrils is prevented by Erk1/2 activation under high calcium conditions Connect2007

    • Author(s)
      Fujisaki H, Ebihara T, Irie S, Kobayashi T, Adachi E, Mochitate K, and Hattori S
    • Journal Title

      Tissue Res. 48(3)

      Pages: 159-169

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of an acyl-CoA synthetase, lipidosin, in astrocytes of the murine brain and its up-regulation during remyelination following cuprizone-induced demyelination2007

    • Author(s)
      Song Si-Young, Kato C, Adachi E, Moriya-Sato A, Inagawa-Ogashiwa M, Umeda R, Hashimoto N
    • Journal Title

      J. Neurosci. Res. 85(16)

      Pages: 3586-3597

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical Distribution of Heat Shock Protein 47 (HSP47) in Scirrhous Carcinoma of the Stomach2006

    • Author(s)
      Hirai K, Kikuchi S, et. al.
    • Journal Title

      Anticancer Research 26

      Pages: 71-78

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Elimination of epiplakin by gene targeting resuts in acceleration of keratinocyte migration in mice2006

    • Author(s)
      Goto Mizuki, Sumiyoshi Hideaki, Sakai Takao, Fossler R, Ohashi S, Adachi E, Yoshioka H, Fujiwara S
    • Journal Title

      Molecular & Cellular Biology 26(2)

      Pages: 548-558

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 12006

    • Author(s)
      Hjrai K, Kikuchi S, et. al.
    • Journal Title

      Anticancer Research 26

      Pages: 71-78

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Immunohistochemical Distribution of Heat Shock Protein 47 (HSP47) in Scirrhous Carcinoma of the Stomach2006

    • Author(s)
      Hirai K, Kikuchi S, et al.
    • Journal Title

      Anticancer Research 26

      Pages: 71-78

    • Related Report
      2006 Annual Research Report
  • [Presentation] Restoration to a contractile phenotype from dedifferentiated status of smooth muscle cells in layered vascular constructs2007

    • Author(s)
      Eijiro Adachi, Takashi Aihara, Satoshi Hosoya and Hironobu Iwashiro
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      アメリカ
    • Related Report
      2008 Final Research Report
  • [Presentation] スキルス胃癌微小環境の再生と線維化制御の意義について2006

    • Author(s)
      平井和弥、菊池史郎、栗田敦、安達栄治郎、渡邊昌彦
    • Organizer
      第78回日本胃癌学会
    • Place of Presentation
      横浜
    • Related Report
      2008 Final Research Report
  • [Presentation] 細胞外マトリックスの生物学とバイオマテリアル足場2006

    • Author(s)
      安達栄治郎
    • Organizer
      第9回日本組織工学会
    • Place of Presentation
      京都(抄録集 p.80)
    • Related Report
      2008 Final Research Report
  • [Book] 細胞外マトリックスの生物学2006

    • Author(s)
      安達栄治郎、相原孝至、大橋しほ花、浅野仁美、水野一乘
    • Publisher
      ティッシュエンジニアリング, 日本医学館, 東京
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] コラーゲン特異的分子シャペロンHSP47の測定方法2008

    • Inventor(s)
      渡邊昌彦, 菊池史郎他4名
    • Industrial Property Rights Holder
      学校法人北里学園
    • Patent Publication Number
      2008-122276
    • Acquisition Date
      2008-05-29
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] 高密度培養組織の製造方法及び高密度培養組織2006

    • Inventor(s)
      安達栄治郎、大橋しほ花、他1名
    • Industrial Property Rights Holder
      学校法人北里学園
    • Acquisition Date
      2006-08-24
    • Related Report
      2008 Final Research Report
  • [Patent(Industrial Property Rights)] コラーゲン特異的分子シャペロンHSP47の測定方法2006

    • Inventor(s)
      渡邊 昌彦 他
    • Industrial Property Rights Holder
      学校法人北里学園
    • Industrial Property Number
      2006-307831
    • Filing Date
      2006-11-14
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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