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Identification of SOX4 related with Colon Development and Carcinogenesis, and Vaccination

Research Project

Project/Area Number 18591491
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionTazuke Kofukai Medical Research Institute

Principal Investigator

HIROKI Hashida  Tazuke Kofukai Medical Research Institute, 2 Division, Medical Research Institute, Resrarcher (70281607)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsSOX4 / Colon Cancer / Colon Development / Wnt Signaling / Vaccine Therapy
Research Abstract

Gene expression profiling of embryonic mouse colon development was performed to identify regulated genes whose misexpression might associate with colon cancer Transcription factor SOX4 was identified as the one of top genes whose expression down-regulated as colon undergoes developmental maturation from undifferentiated endoderm to a more differentiated polarized epithelium (embryonic day 13.5-18.5). Its expression was increased in human colon adenomas compared to normal colon mucosa and its expression increased more than that during colon cancer development SOX4 expression was found nucleus and /or cytoplasm in colon cancer cells as various patterns. In human colon cancer cells with APC mutation, endogenous SOX4 and beta-catenin interact, co-localize in nucleus partially.
In the serial analysis of gene expression (SAGE) database, SOX4 expression was increased in colon cancer cell lines and actual colon cancer tissues compared to human normal colon tissues. SOX4 transfectants were produced using HEK293 cells and several colon cancer cell lines (SW480, HT29 etc). The luciferase assay showed that SOX4 transfectants regulated the Wnt signaling to stabilize beta-catenin.
In addition, to examine the potential of SOX4 for broad use as a colon cancer vaccine and the immunological potential, SOX4 specific CD4 and CD8 T cells were evaluated. Our preliminary study demonstrated that the identification of natural processed T cell from SOX4 provided development of peptide-based vaccination therapy for colon cancer.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (14 results)

All 2008 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (5 results)

  • [Journal Article] Prognostic significance of the immediate early response gene X-1 (IEX-1) expression in pancreatic cancer.2008

    • Author(s)
      Sasada T., et. al.
    • Journal Title

      Annals of Surgical Oncology 15

      Pages: 609-617

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prognostic significance of the immediate early response gene X-1(IEX-1) expression in pancreatic cancer2008

    • Author(s)
      Sasada T, Azuma K, Hirai T, Hashida H, Kanai M, Yanagawa T, Takabayashi A.
    • Journal Title

      Annals of Surgical Oncology. 15(2)

      Pages: 609-617

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prognostic significance of the immediate early response gene X-1(IEX-1)expression in pancreatic cancer2008

    • Author(s)
      Sasada T., et. al.
    • Journal Title

      Annals of Surgical Oncology 15

      Pages: 609-617

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SMAD4-deficient intestinal tumors recruit CCR1+ myeloid cells that promote invasion.2007

    • Author(s)
      Kitamura T., et. al.
    • Journal Title

      Nature Genetics 39

      Pages: 467-475

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 膵管出血をきたした退形成性膵管癌の1例2007

    • Author(s)
      水上陽, 他
    • Journal Title

      膵臓 22

      Pages: 488-449

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] SMAD4-deficient intestinal tumors recruit CCR1+myeloid cells that promote invasion.2007

    • Author(s)
      Kitamura T, Kometani K, Hashida H, Matsunaga A, Miyoshi H, Hosogi H, Aoki M, Oshima M, Hattori M, Takabayashi A, Minato N, Taketo MM.
    • Journal Title

      Nature Genetics. 39(4)

      Pages: 467-475

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] A case of giant cell type anaplastic carcinoma of the pancreas with hemosuccus pacreaticus.2007

    • Author(s)
      Mizukami Y, Sasada T, Hashida H, Mizumoto A, Satoh M, Ueda S, Tanaka M, Gunji S, Oishi M, Koyama Y, Yoshida M, Kanai M, Takasu K, Takabayashi A.
    • Journal Title

      Suizo. 22(4)

      Pages: 488-493

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] SMAD4-deficient intestinal tumors recruit CCR1+ myeloid cells that promote invasion2007

    • Author(s)
      Kitamura T., et. al.
    • Journal Title

      Nature Genetics 39

      Pages: 467-475

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SMAD4-deficient intestinal tumors recruit CCR1+ myeloid cells that promote invasion.2007

    • Author(s)
      Kitamura T, Kometani K, Hashida H, Matsunaga A, Miyoshi H, Hosogi H, Aoki M, Oshima M, Hattori M, Takabayashi A, Minato N, Taketo MM
    • Journal Title

      Nature Genetics 39(4)

      Pages: 467-475

    • Related Report
      2006 Annual Research Report
  • [Presentation] リンパ節転移陽性直腸癌に対する術前放射線化学療法の有用性2007

    • Author(s)
      橋田裕毅
    • Organizer
      第62回日本消化器外科学会定期学術総会
    • Place of Presentation
      東京
    • Year and Date
      2007-07-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Efficacy of preoperative chemoradiotherapy for advaced rectal cancer.2007

    • Author(s)
      Hashida H, Mizumoto A, Mizukami Y, Yoshida M, Koyama Y, Gunji S, Tanaka M, Ueda S, Kanai M, Takabayashi A.
    • Organizer
      The 62nd Annual Meeting of the Japanese Society of gastroenterological Surgery
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-07-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 血行改変を用いた切除不能酔膵癌に対する選択的動注化学療法2007

    • Author(s)
      橋田裕毅
    • Organizer
      第107回日本外科学会定期学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2007-04-13
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Arterial infusion chemotherapy for advanced pancreatic carcinoma after vascular reconstruction.2006

    • Author(s)
      Hashida H, Sasada T, Mizukami Y, Oishi M, Koyama Y, Gunji S, Yoshida M, Tanaka M, Ueda S, Kanai M, Takabayashi A.
    • Organizer
      The 107th Annual Congress of Japan Surgical Society
    • Place of Presentation
      Osaka
    • Year and Date
      2006-04-13
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Indentification of SOX4 related with colon development and carcinogenesis.2006

    • Author(s)
      Hashida H, Ueda S, Sasada T, Mizumoto A, Denno R, Kanai M, Takabayashi A.
    • Organizer
      The 106th Annual Congress of Japan Surgical Society
    • Place of Presentation
      Tokyo
    • Year and Date
      2006-03-31
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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