• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The mechanism and clinical significance of loss of CEACAM1 expression in hepatocellular carcinoma

Research Project

Project/Area Number 18591504
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionNiigata University

Principal Investigator

WAKAI Toshifumi  Niigata University, Medical and Dental Hospital, Assistant Professor (50372470)

Co-Investigator(Kenkyū-buntansha) SHIRAI Yoshio  Niigata University, Graduate School of Medical and Dental Sciences, Associate Professor (50216173)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsCEACAM1 / HepG2 / Anchorage-independent growth / small interfeing RNA / Hepatocellular carcinoma / Small interfering RNA / Isoform / Neoplasm metastasis / Transmembrane domain / Cytoplasmic domain
Research Abstract

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), an adhesion molecule of the immunoglobulin superfamily, has been characterized as a putative tumor suppressor because it is frequently down-regulated in aggressive types of cancer cells. Recently, however, several studies have shown that CEACAM1 actively contributes to malignant progression or migration in some types of tumor cells, suggesting that the role of CEACAM1 might be diverse among different types of cancer cells. To investigate the functional consequences of CEACAM 1 expression in hepatocellular carcinoma, we analyzed the status of CEACAM1 in hepatoma cell lines HLF, PLC/PRF/5, HepG2 and KYN-2. We found that CEACAM1 was only expressed in HepG2 cells, which show a unique property for enhanced anchorage-independent growth. When HepG2 cells were treated with small interfering RNA targeted against CEACAM1, the growth rate in monolayer culture was increased. In contrast, when HepG2 cells were cultured in suspension, inhibition of CEACAM1 expression significantly decreased the growth rate, and the speed of cell-cell attachment was repressed. Hyaluronidase treatment attenuated the growth rate of HepG2 cells in suspension culture, indicating that cell-cell attachment is a requisite for anchorage-independent growth. Our data may reveal the dual role of CEACAM1 on hepatocarcinogenesis, by showing that CEACAM1 acts as a tumor suppressor in HepG2 cells in anchorage-dependent growth conditions, while in anchorage-independent growth conditions, it augments cell proliferation by potentiating the cell-cell attachment.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2007

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] Tumor suppressor carcinoembryonic antigen-related cell adhesion molecule 1 potentates the anchorage-independent growth of human hepatoma HepG2 cells.2007

    • Author(s)
      Hokari M, Matsuda Y, Wakai T, et. al.
    • Journal Title

      Life Science 81

      Pages: 336-345

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Anatomic resection independently improves long-term survival in patients with T1-T2 hepatocellular carcinoma.2007

    • Author(s)
      Wakai T, Shirai Y, Sakata J, et. al.
    • Journal Title

      Annals of Surgical Oncology 14

      Pages: 1356-1365

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Long-term outcomes after hepatectomy for recurrences after prior local ablation for hepatocellular carcinoma.2007

    • Author(s)
      Sakata J, Shirai Y, Wakai T, et. al.
    • Journal Title

      European Journal of Surgical Oncology

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Tumor suppressor carcinoembryonic antigen-related cell adhesion molecule 1 potentates the anchorage-independent growth of human hepatoma HepG2 cells2007

    • Author(s)
      Hokari, M, Matsuda, Y, Wakai, T, et. al.
    • Journal Title

      Life Science 81

      Pages: 336-345

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Anatomic resection independently improves long-term survival in patients with T1-T2 hepatocellular carcinoma.2007

    • Author(s)
      Wakai, T, Shirai, Y, Sakata, J, et. al.
    • Journal Title

      Annals of Surgical Oncology 14

      Pages: 1356-1365

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Long-term outcomes after hepatectomy for recurrences after prior local ablation for hepatocellular carcinoma.2007

    • Author(s)
      Sakata, J, Shirai, Y, Wakai, T, et. al.
    • Journal Title

      European Journal of Surgical Oncology, PMID 17475439

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 脈管侵襲を伴う肝細胞癌における細胞接着因子の臨床的意義2007

    • Author(s)
      Pavel Korita, 若井俊文, 白井良夫, 他
    • Organizer
      第107回日本外科学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-04-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Clinical significance of cell adhesion molecules in hepatocellular carcinoma with vascular invasion2007

    • Author(s)
      Korita, P, Wakai, T, Shirai, Y, et. al.
    • Organizer
      The 107th Annual Congress of Japan Surgical Society
    • Place of Presentation
      Osaka
    • Year and Date
      2007-04-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi