| Project/Area Number |
18591513
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
UYAMA Naoki Hyogo College of Medicine, Faculty of Medicine, Professor (70402873)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Jiro Hyogo College of Medicine, Faculty of Medicine, Professor (90199373)
IIMURO Yuji Hyogo College of Medicine, Faculty of Medicine, Associate Professor (30252018)
KAWADA Norifumi Osaka City University, Graduate school of Medine, Faculty of Medicine, Professor (30271191)
IKEDA Kazuo Osaka City University, Graduate school of Medine, Faculty of Medicine, Associate Professor (80275247)
HATANO Etsuro Kyoto University, Graduate school of Medine, Faculty of Medicine, Instructor (80359801)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Intermediate filament / liver fibrois / hepatic stellate cells / hepatocellular carcinoma / synemin / vimentin |
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| Research Abstract |
(A) Expression of Intermediate filament proteins by rat hepatic stellate cells.
Expression of intermediated filament proteins in rat normal or cirrhotic liver tissues was investigated. In normal liver, GFAP, desmin, vimentin and synemin were expressed by hepatic stellate cells. In cirrhotic liver, expression of GFAP and synemin was down-regulated and expression of desmin and vimentin was up-regulated. Isolated stellate cells from rat liver are thought to be activated during the culture on the plastic dish. Expression of GFAP and synemin was down-regulated and expression of desmin and vimentin was up-regulated during the culture. So, we found that quiescent rat stellate cells express synmin and GFAP and activated stellate cells express desmin and vimentin.
(B) Expression of Intermediate filament proteins by human hepatic stellate cells.
Expression of GFAP, synemin, desmin and vimentin in human normal or cirrhotic liver tissues was investigated. In normal liver, vimentin and synemin were expressed by hepatic stellate cells. However, desmin and GFAP were not expressed by hepatic stellate cells. In cirrhotic liver, expression of synemin was down-regulated and expression of vimentin was up-regulated. In LX-2 cells, cell line of stellate cells, Expression of vimentin, but not synemin, detected. So, we found that synmin and vimentin play a key role to regulate the activation of hepatic stellate cells.
(C) The role of vimentin in human hepatic stellate cells.
We investigated the role of vimentin in human hepatic stellate cells. Expression of vimentin in LX-2 cells were down regulated by 85% of control by siRNAs. Depletion of vimentin did not affect the ability to proliferate and the expression of type I collagen. In the future, we will investigate the function of vimentin in human hepatic stellate cells again by other methods.
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