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Role of bone marrow derived cells in progression of hepatic fibrosis.

Research Project

Project/Area Number 18591520
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionFukushima Medical University

Principal Investigator

TSUCHIYA Takao  Fukushima Medical University, Department of Surgery I, Faculty of Medicine, Associate professor (70343390)

Co-Investigator(Kenkyū-buntansha) SAITO Takurou  Fukushima Medical University, Department of Surgery I, Faculty of Medicine, Associate professor (20305361)
KENJO Akira  Fukushima Medical University, Department of Surgery I, Faculty of Medicine, Associate professor (40305355)
KIMURA Takashi  Fukushima Medical University, Department of Surgery I, Faculty of Medicine, Associate professor (00381369)
TERASHIMA Masanori  Fukushima Medical University, Department of Surgery I, Faculty of Medicine, Assistant professor (40197794)
GOTOH Mitsukazu  Fukushima Medical University, Department of Surgery I, Faculty of Medicine, Professor and director (50162160)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsliver fibrosis / hepatic regeneration / hone marrow cell / green mouse
Research Abstract

Bone marrow derived cells are involved in the process of hepatic fibrosis secondary to chronic injury. However, it is not yet known how quickly this event occurs in acute fibrosis models. The purpose of this study was to determine the role of bone marrow cells in rapid fibrosis following bile duct ligation in mice using chimeric green fluorescent protein expressing bone marrow cells.
METHODOLOGY : After whole body irradiation, 1 × 106 donor whole bone marrow cells from green fluorescent protein^+/-mice were transplanted into C57BL/6 recipients via the tail vein. Four weeks after bone marrow transplantation, chimeric mice were subjected to common bile duct ligation, and livers of these animals were histologically examined after bile duct ligation using anti-fibroblast specific protein-1 antibody and anti-alpha-smooth muscle actin antibody.
RESULTS : Periductal fibrosis consisting of fibroblast specific protein-positive cells was demonstrated histologically as early as day 7. Most of the fibrotic cells were green fluorescent protein-negative, however, a significant number of cells (10%) were green fluorescent protein-positive and some were also anti-fibroblast specific protein-1- or alpha-smooth muscle actin-positive.
CONCLUSIONS : Differentiation of bone marrow derived cells to activated fibroblast and myofibroblast-like phenotypes occurs in the very early course of periductal fibrosis following bile duct ligation.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 Other

All Journal Article (14 results) (of which Peer Reviewed: 4 results) Presentation (6 results)

  • [Journal Article] Bone marrow traffic to regenerating islets induced by streptozotocin injection and partial pancreatectomy in mice2008

    • Author(s)
      Ohtake K, Saito T, Satoh Y, Keno A, Kimura T, Asawa S, Anazawa T, Gotoh M.
    • Journal Title

      Transplant Proc 40

      Pages: 449-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Traffic of infused bone marrow cells after genetically-labeled syngeneic bone marrow transplantation following lethal irradiation in mice2008

    • Author(s)
      Satoh A, Saito S, Sato Y, Tsuchiya T, Kenio A, Kimura T, Kanno R, Suzuki H, Kogure M, Hoshino Y, Gotoh M.
    • Journal Title

      Fukushima Journal of Medical Science (In press)

    • NAID

      10030915749

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bone marrow traffic to regenerating islets induced by streptozotocin injection and partial pancreatectomy in mice.2008

    • Author(s)
      Ohtake, K., Saito, T., Satoh, Y., Kenjo, A., Kimura, T., Asawa, S., Anazawa, T., Gotoh, M
    • Journal Title

      Transplant Proc. 40(2)

      Pages: 449-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Participation of bone marrow cells in biliary fibrosis after bile duct ligation2007

    • Author(s)
      Asawa S, Saito T, Satoh A, OhtakeK, TsuchiyaT, OkadaH, Neilson EG, Gotoh M.
    • Journal Title

      J Gastroenterol Hepatol 22

      Pages: 2001-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Ischemic preconditioning enhances regenerative capacity of hepatocytes in long-term is chemically damaged rat livers2007

    • Author(s)
      Yamada F, Saito T, Abe T, Tsuchiya T, Sato Y, Kenjo A, Kimura T, Gotoh M.
    • Journal Title

      J Gastroenterol Hepatol 22

      Pages: 1971-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Participation of bone marrow cells in biliary fibrosis after bile duct-ligation.2007

    • Author(s)
      Asawa, S., Saito, T., Satoh, A., Ohtake, K., Tsuchiya, T., Okada, H., Neilson, EG., Gotoh, M
    • Journal Title

      J Gastroenterol Hepatol 22(11)

      Pages: 2001-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Ischemic preconditioning enhances regenerative capacity of hepatocytes in long-term ischemically damaged rat livers.2007

    • Author(s)
      Yamada, F., Saito, T., Abe, T., Tsuchiya, T., Sato, Y., Kenjo, A., Kimura, T., Gotoh M
    • Journal Title

      J Gastroenterol Hepatol 22(11)

      Pages: 1971-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Participation of bone marrow cells in biliary fibrosis after bile ductligation2007

    • Author(s)
      斎藤拓朗、土屋貴男、見城明、木村隆、後藤満一
    • Journal Title

      J Gastroenterol Hepatol 22(11)

      Pages: 2001-8

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Ischemic preconditioning enhances regeneration capacity of hepatocytes in long- term ischemically damaged rat livers2007

    • Author(s)
      斎藤拓朗、土屋貴男、見城明、木村隆、後藤満一
    • Journal Title

      J Gastroenterol Hepatol 22(11)

      Pages: 1971-77

    • Related Report
      2007 Annual Research Report
  • [Journal Article] 小腸移植2006

    • Author(s)
      土屋貴男, 斉藤拓朗, 見城明, 木村隆, 後藤満一
    • Journal Title

      綜合臨床 55・8

      Pages: 2077-2085

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 免疫不全状態での抗菌薬療法-肝移植後2006

    • Author(s)
      土屋貴男, 斉藤拓朗, 見城明, 木村隆, 後藤満一
    • Journal Title

      小児内科 38・7

      Pages: 1199-1202

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Traffic of infused bone marrow cells after genetically-abeled syngeneic bone marrow transplantation following lethal irradiation in mice

    • Author(s)
      Satoh A., Saito T., Sato Y., Tsuchiya T., Kenjo A., Kimura T., Kanno R., Suzuki H., Kogure M., Hoshino Y., Gotoh M
    • Journal Title

      Fukushima J Med Sci (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Participation of bone marrow cells in biliary fibrosis after bile duct ligation

    • Author(s)
      斉藤拓朗, 土屋貴男, 見城明, 木村隆, 後藤満一
    • Journal Title

      Hepatology (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ischemic preconditioning enhances regeneration capacity of hepatocytes in long- term ischemically damaged rat livers

    • Author(s)
      斉藤拓朗, 土屋貴男, 見城明, 木村隆, 後藤満一
    • Journal Title

      Hepatology (In press)

    • Related Report
      2006 Annual Research Report
  • [Presentation] Participation of bone marrow derived cells in islet regeneration after streptozotoc ininjection and partial hepatectomy in mice2007

    • Author(s)
      Ohtake K.
    • Organizer
      CTS-IPITA-IXA2007 Joint conference
    • Place of Presentation
      Minneapolis,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Participation of bone marrow derived cells in islet regeneration after streptozotocin injection and partial hepatectomy in mice.2007

    • Author(s)
      Ohtake, K., Saito, T., Satoh, Y., Kenjo, A., Kimura, T., Asawa, S., Anazawa, T., Gotoh, M
    • Organizer
      CTS-IPITA-IXA2007 Joint conference
    • Place of Presentation
      Minneapolis, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 肝線維化における骨髄由来細胞の関与に関する研究2006

    • Author(s)
      浅和 定徳
    • Organizer
      第402回福島医学会
    • Place of Presentation
      福島市
    • Year and Date
      2006-10-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 長時間肝温虚血再灌流障害に対するIschemic preconditioningの効果2006

    • Author(s)
      山田 文彦
    • Organizer
      第402回福島医学会
    • Place of Presentation
      福島市
    • Year and Date
      2006-10-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The role of bone marrow derived cells in progression of hepatic fibrosis after bile duct ligation.2006

    • Author(s)
      Sadanori, Asawa
    • Organizer
      Congress of the Fukushima Society of Medical Science
    • Place of Presentation
      Fumihiko, Yamada
    • Year and Date
      2006-10-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Ischemic preconditioning enhances regenerative capacity of hepatocytes in long-term ischemically damaged rat livers.2006

    • Organizer
      Congress of the Fukushima Society of Medical Science.
    • Year and Date
      2006-10-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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