| Project/Area Number |
18591523
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KAWAI Manabu Wakayama Medical University, School of Medicine, Second Department of Surgery, Assistant (40398459)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAUE Hiroki Wakayama Medical University, School of Medicine, Second Department of Surgery, Professor (20191190)
UCHIYAMA Kazuhisa Wakayama Medical University, School of Medicine, Second Department of Surgery, Assistant Professor (80232867)
TANI Masaji Wakayama Medical University, School of Medicine, Second Department of Surgery, Instructor (60236677)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,840,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | intraductal papillary-mucinous neoplasms / pure pancreatic juice / molecular diagnosis / malignant diagnosis / 網羅的遺伝子解析 |
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| Research Abstract |
Background: The early diagnosis of malignant intraductal papillary mucinous neoplasms (IPMN) still remains controversial as IPMT shows a wide spectrum of histological characteristics ranging from hyperplasia to invasive carcinoma. Cytological examination of pure pancreatic juice, obtained by endoscopic retrograde pancreatography (ERP) has been performed for patients with pancreatic neoplasms. However, its sensitivity is very low. The aim of the present study was to determine the new molecular method for the early diagnosis of malignant IPMN. Patients and Method: Thirty four consecutive patients with 29 pancreatic neoplasms (13 invasive adenocarcinoma, 9 intraductal papillary mucinous carcinoma, 7 intraductal papillary mucinous adenoma) and 5 chronic pancreatitis were enrolled. Pure pancreatic juice was obtained by ERP, and performed a quantitative detection method using a real-time reverse transcriptase chain reaction (RT PCR) system with hybridization probes. The mRNA Levels of CEA an
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d MUC1 were standardized to GAPDH. This methods could reproducibly quantify 10 to 10^6 Carcinoembryonic antigen (CEA) and 10^2 to 10^6 membrane-bound type mucin 1 (MUC1) expressing pancreatic caricinoma cells (PK-8) in 10^7 peripheral blood mononuclear cells. Results: MUC1 mRNA in pure pancreatic juice was detected at a significant higher frequency in malignant IPMN compared to benign IPMN (1, 079±879 vs 6, 938±7, 930, p=0.0229). However, CEA mRNA in pure pancreatic juice was not detected at a significant higher frequency in malignant IPMN compared to benign IPMN (25.5±21.5 vs 758±1, 824, p=0.0641). The optimal cut-off levels for MUC1 mRNA in pure pancreatic juice for differentiation between benign IPMN and malignant IPMN were sought by constructing receiver operating characteristics (ROC) curve. The cut-off level of MUC1 mRNA in pure pancreatic juice was at 1, 600 for differentiation between benign IPMN from malignant IPMN. The sensitivity of MUC1 mRNA cut-off line was 88.9%, the specificity was 71.4%. The accuracy was determined to be 81.3%. Conclusions: These results suggested that a quantitative detection of MUC1 mRNA in pure pancreatic juice is the useful preoperative diagnosis of malignant IPMN. Less
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