| Project/Area Number |
18591529
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Asahi University (2007)
Osaka Medical College (2006) |
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Principal Investigator |
TAKAORI Kyoichi Asahi University, Department of Surgery, Professor (10329485)
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| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Surgery / Pathology / International collaboration / Cancer / 膵管内腫瘍性病変 / pancreatic intraepithelial neoplasia / PanIN / 膵癌前駆病変 / レーザーキャプチャー / マイクロダイセクション |
|---|
| Research Abstract |
Pancreatic intraepithelial neoplasia (PanIN) is a microscopic lesion identified in the pancreatic duct system and PanIN is presumed to be a precursor to invasive pancreatic carcinoma. However, the pathways from PaniN toward invasive pancreatic carcinoma have not been thoroughly studied so far. The aim of this study was to investigate the pathways from PanIN toward invasive pancreatic carcinoma by complimentary genomic hybridization. Surgical specimens of the pancreas from five patients with invasive pancreatic carcinoma, three with intraductal papillary mucinous neoplasm, two with gastric cancer were used under guidance of ethical codes. Cellular content of PanIN lesions were retrieved by laser micro-dissection under observation by a microscope (PixCell LCM, Aicturus, USA). DNA was isolated for further analysis. Preliminary experiment for degenerate oligonucleotide primed-PCR and complimentary genomic hybridization was carried out.
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