| Project/Area Number |
18591535
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
KAGA Kichizo Hokkaido University, Hokkaido University Hospital, Lecture (80224335)
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| Co-Investigator(Kenkyū-buntansha) |
HIDA Yasuhiro Hokkaido University, Hokkaido University Hospital, Lecturer (30399919)
KONDO Satoshi Satoshi Hokkaido University, Graduate School of medicine, Professor (30215454)
MIYAMOTO Masaki Masaki Hokkaido University, Hokkaido University Hospital, Research (40333611)
HIDA Kyoko Hokkaido University, Hokkaido University Hospital, research assistant (40399952)
SHICHINOHE Toshiaki Toshiaki Hokkaido University, Graduate School of medicine, Lecture (70374353)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | cancer / EPC / PBMC / anqioqenesis / tumor marker / 血管内皮前駆細胞 / 末梢血 / 腫瘍血管内皮細胞 |
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| Research Abstract |
Endothelial progenitor cell (EPC) is known as a significant material of angiogenesis in the tumor. EPC is released from the bone marrow and the number depends on the mediators secreted by tumor cells. Therefore, we hypothesized that EPC would show the status of tumor growth and indicates the prognosis of cancer patients. We constructed PEDF expressing lentivirus vector (Lv-PEDF) and established PEDF-overexpressiong cell lines HEC46-PEDF and TE8-PEDF. HEC46-PEDF and TE8-PEDF were implanted into nude mice subcutaneously and the growth was monitored After then, PBMC was extracted and the number of EPC was counted by using FACS. The number of EPC was increased in the mice with wild type HEC46 tumors or TE8 tumors, but not HEC46-PEDF and TE8-PEDF. Therefore, the data suggested that tumor cells secret factors inducing angiogenesis and they would be inhibited by PEDF Subsequently, we extracted PBMC from patients with tumor and assessed the number of PBMC and EPC.
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