Exhaustive investigation of gene expression profile in early carcinogenesis of human lung adenocarcinoma
| Project/Area Number |
18591539
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
ANAMI Yoichi University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant professor (40317376)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Masayuki University of Tsukuba, Graduate School of Comprehensive Human Sciences, Professor (00198582)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | immortalized cell lines / atypical adenomatous hyperplasia(AAH) / cDNA microarray / small adenocarcinoma / Sushi domain containing 2(SUSD2) / Neuromedin U(MNU) / Neuromadin U / prognostic factor / 異型腺腫様過形成(AAH)不死化細胞株 / 細気管支肺胞上皮癌 / 混合型腺癌 / real-time PCR / Neuromedin U |
|---|
| Research Abstract |
We have already had two immortalized cell lines which are developed from atypical adenomatous hyperplasia (AAH) and its counterpart normal bronchial epithelium, respectively. As development of molecular biology, it was enable to detect expression levels of thousands of genes on cDNA microarray. We aimed to detect significant genes which have important roles in early carcinogenesis, using these two cell lines and cDNA microarray. We detected that Sushi domain containing 2 (SUSD2) and Neuromedin U (MNU) were overexpressed more than 10 times in AAH cell line compared with normal bronchial epithelial cell line. We revealed that SUSD2 was overexpressed in BAC and NMIU was overexpressed in early invasive cancer compared with normal lung tissue. And finally, we revealed that patients with small sized adenocarcinomas with positeve staining for SUSD2 showed long survival time, and SUSD2 expression was significantly related to preferable survival (p=0.0112).
|
Report
(3 results)
Research Products
(62 results)
