Transplantation of primate embryonic stem cell-derived neural stem cell against brain ischemia

• Project/Area Number: 18591588
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Kyoto University
• Principal Investigator: TAKAGI Yasushi Kyoto University, Graduate School of Medicine, Lecturer (40312227)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000); Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: ES cell / brain ischemia / regenerative therapy / primate / 神経幹細胞 / 移植

Research Abstract

It became possible that we can make neural stem cell from embryonic stem cells. Now we can stat to experimental transplantation aginst brain ischemia. We assessed the result of transplantation against mouse focal ischemaia model using monkey ES cells. We analyzed the survival of transplanted cells at 14 days and 28 days after ischemia. In addition, we analyzed matrix metalloproteinase expressions and tissue inhibitor of metalloproteinases after ischemis. We employed TIMP-1 and TIMP-2 knockout mice. Furthermore we analyzed the effect of heatshock protein110during brain ischemia.

Research Products

• [Journal Article] Expression of hypoxia-inducing factor-1 alpha and endoglin in intimal hyperplasia of the middle cerebral artery of patients with Moyamoya disease. (2007)
  • Author(s): Takagi Y, et al.
  • Journal Title: Neurosurgery 60 Pages: 338-345
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Neuronal expression of Fas-associated death domain protein and caspase-8 in the perinidal parenchyma of cerebral arteriovenous malformations (2007)
  • Author(s): Takagi Y, et al.
  • Journal Title: J Neurosurg 106 Pages: 275-282
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cerebral aneurysm progression suppressed by blockage of endothelin Breceptor. (2007)
  • Author(s): Sadamasa N, et al.
  • Journal Title: J Neurosurg 106 Pages: 330-336
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] pression of hypoxia-inducing factor-1 alpha and endoglin in intimal hyperplasia of the middle cerebral artery of patients with Moyamoya disease. (2007)
  • Author(s): Takagi Y, Kikuta K, Nozaki K, Fujimoto M, Hayashi J, Imamura H, Hashimoto N.
  • Journal Title: Neurosurgery. 60(2) Pages: 338-45
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Neuronal expression of Fas-associated death domain protein and caspase-8 in the perinidal parenchyma of cerebral arteriovenous malformations. (2007)
  • Author(s): Takagi Y, Kikuta K, Nozaki K, Fujimoto M, Hayashi J, Hashimoto N
  • Journal Title: J Neurosurg. 106(2) Pages: 275-82
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cerebral aneurysm progression suppressed by blockage of endothelin B receptor. (2007)
  • Author(s): Sadamasa N, Nozaki K, Takagi Y, Moriwaki T, Kawanabe Y, Ishikawa M, Hashimoto N.
  • Journal Title: J Neurosurg. 106(2) Pages: 330-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 脳梗塞に対する再生医療-現状と展望- (2007)
  • Author(s): 高木 康志
  • Journal Title: 脳神経外科ジャーナル Pages: 481-489
  • NAID: 110006279434
  • Peer Reviewed
• [Journal Article] Primate embryonic stem cell-derived neuronal progenitors transplanted into ischemic brain (2006)
  • Author(s): Hayashi J, Takagi Y, Fukuda H, Imazato T, Nishimura M et al.
  • Journal Title: J Cereb Blood Flow Metab 26(7) Pages: 906-914
• [Journal Article] 脳梗塞に対する細胞移植治療への期待-脳神経疾患への細胞移植治療の現状を含めて- (2006)
  • Author(s): 高木康志
  • Journal Title: 脳循環代謝 18 Pages: 104-109
• [Journal Article] ES細胞を用いた脳梗塞治療 (2006)
  • Author(s): 高木康志
  • Journal Title: 分子血管病 5(4) Pages: 414-419
• [Journal Article] 脳梗塞に対するES細胞由来神経幹細胞の移植 (2006)
  • Author(s): 高木康志, 橋本信夫
  • Journal Title: 脳卒中 28 Pages: 600-605
  • NAID: 10018686834
• [Presentation] 幹細胞移植とその臨床応用 (2007)
  • Author(s): 高木康志
  • Organizer: 第19回日本脳循環代謝学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2007-10-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Transplantation of stem cells and clinical applications (2007)
  • Author(s): Yasushi Takagi
  • Organizer: 19^<th> annual meeting of Japanese society of cerebral blood flow and metabolism
  • Place of Presentation: Morioka
  • Year and Date: 2007-10-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 脳梗塞に対する再生医療 (2007)
  • Author(s): 高木 康志
  • Organizer: 日本脳循環代謝学会
  • Place of Presentation: 盛岡市
  • Year and Date: 2007-10-26