| Project/Area Number |
18591596
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
NAGAYAMA Tetsuya Kagoshima University, Medical and Dental Hospital, Senior Assistant Professor (40336334)
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| Co-Investigator(Kenkyū-buntansha) |
NIIRO Masaki Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor (30172612)
MIYATA Atsuro Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (60183969)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,860,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | C-type natriuretic peptide / PACAP / ischemia / astrocytes / neuronal cell / endothelial cell / RIA / RT-PCR |
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| Research Abstract |
C-type natriuretic peptide (CNP) belongs to the natriuretic peptide family and has a ring structure formed by an intramolecular disulfide bond, which is conserved among atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP),and CNP. ANP and BNP are mainly secreted from heart as cardiac hormones, whereas CNP is mainly localized in the central nervous system (CNS),but the function of CNP in the CNS has not been clarified so far. Receptors for natriuretic peptides are mainly guanylate cyclase-A (GC-A) and guanylate cyclase-B (GC-B). ANP and BNP show high affinity to GC-A, whereas, CNP preferentially binds to GC-B. Both GC-A and GC-B elicit an increase in intracellular cGMP that mediates most of the biological actions of natriuretic peptides. It has been reported that the neurotrophic peptide pituitary adenylate cyclase-activating polypeptide (PACAP)s timulates adenylate cyclase activity in cultured astrocytes. During the course of a study regarding the effect of PACAP on astrocytes with DNA microarray, we found that CNP gene expression was markedly increased in astrocytes stimulated by PACAP. Then, we tried to clarify how PACAP regulates the biosynthesis and secretion of CNP in the astrocytes. In rat-cultured astrocytes, pituitary PACAP activates gene expression and secretion of CNP in a dose- and time-dependent manner. These results suggest that PACAP might be involved in,the regulation of CNP biosynthesis in astrocytes.
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