| Project/Area Number |
18591621
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Akita University |
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Principal Investigator |
OKADA Kyoji Akita University, School of Medicine, Associate professor (10185431)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 周 秋田大学, 医学部, 助手 (90344754)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Acridine orange / Ultrasound / Sarcoma / U1trasound / sarcoma |
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| Research Abstract |
Background: Sonodynamic synergistic antitumor therapy is a promising new methodology for cancer treatment.
Materials and Methods: To determine the antitumor effects of ultrasound (US) in the presence of acridine orange (AO), 1.0 μg/ml solution of AO was tested as a sonodynamic compound against sarcoma 180 cells in vitro.
Results: After US irradiation at 2.0 W/cm^2 for 60 sec, the survival rate of tumor cells in the presence of AO was significantly lower than in its absence (p<0.001). In the AO-treated group, the tumor cells were mostly fragmented. When D-mannitol or L-histidine was used along with the AO, the survival rate of tumor cells after irradiation was significantly higher than that when AO alone was applied.
Conclusion: AO could exhibit useful antitumor activity under US irradiation, and the generation of singlet oxygen and hydroxyl radicals is involved in the processes of tumor cell damage.
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