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The study for the regenerative med nc of articular cartilage using cell sheet techonology

Research Project

Project/Area Number 18591644
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionTokai University

Principal Investigator

SATO Masato  Tokai University, Orthopaedic Surgery, Associate Professor (10056335)

Co-Investigator(Kenkyū-buntansha) MOCHIDA Joji  Tokai University School of Medicine, Orthopaedic Surgery, Professor (50174347)
MITANI Genya  Tokai University School of Medicine, Orthopaedic Surgery, Assistant Professor (80307280)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsTemperature-responsive culture dish / Cell sheet / Tissue Engineering / Chondrocyte / Partial thickness defect / Articular cartilage / 軟骨再生 / 軟骨修復
Research Abstract

Some treatments for full thickness defects of articular cartilage, such as cultured chondrocytes transplantation, have already been done. However, to overcome osteoarthritis, we must further study the partial thickness defect of articular cartilage. It is much more difficult to repair a partial thickness defect because few repairing cells can address such injured sites. We herein show that bioengineered layered chondrocyte sheets using emperature-responsive culture dishes may be a potentially useful treatment for partial thickness defects. We evaluated the property of these sheets using real-time PCR and histological findings, and allografted these sheets to evaluate the effect of treatment using a rabbit partial model. In conclusion, layered chondrocyte sheets were able to maintain the cartilageous phenotype, and could be attached to the sites of cartilage damage and which acted as a barrier to prevent a loss of proteoglycan from these sites and to protect them from catabolic factors in the joint.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (11 results)

  • [Journal Article] Effects of growth factors on heparin-carrying polystyrene-coated atelocoll agen scaffold for articular cartilage tissue engineering2007

    • Author(s)
      Sato M, Ishihara M, Ishihara M, Kaneshiro N, Mitani G, Nagai T, Kutsuna T, Asazuma T, Kikuchi M, Mochida J.
    • Journal Title

      J.Biomed.Mater.Res.Part B, Appl.Biomaterials 84

      Pages: 181-188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cultured articular chondrocyte sheets for partial thickness cartilage defects utilizing temperature-responsive culture dishes2007

    • Author(s)
      Kaneshiro N, Sato M, Ishihara M, Mitani G, Sakai H, Kikuchi T, Mochida J
    • Journal Title

      European Cells and Materials 13

      Pages: 87-92

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 細胞シート工学2007

    • Author(s)
      三谷 玄弥, 佐藤 正人, 持田 譲冶
    • Journal Title

      臨床雑誌 整形外科 58

      Pages: 216-217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] articular chondrocytes sheets for partial thickness cartilage defects utilizing temperature-responsive culture dish2007

    • Author(s)
      Kaneshiro, N., Sato, M., Ishihara, M., Mitani, G., Kikuchi, T., Mochida, J
    • Journal Title

      European Cells and Materials Cultured 13

      Pages: 87-92

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering2007

    • Author(s)
      Sato, M., Ishihara, M., Ishihara, M., Kaneshiro, N., Mitani, G., Nagai, T., Kutsuna, T., Asazuma, T., Kikuchi, M., Mochida, J
    • Journal Title

      Journal of biomedical materials research. Part B 83(1)

      Pages: 181-188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of growth factors on heparin-carrying Polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering2007

    • Author(s)
      Sato M, Ishihara M, Ishihara M, Kaneshiro N, Mitani G, Nagai T, Kutsuna T, Asazuma T, Kikuchi M, Mochida J.
    • Journal Title

      J.Biomed.Mater.Res.Part B, Appl.Biomaterials 84

      Pages: 181-188

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞シート工学2007

    • Author(s)
      三谷 玄弥, 佐藤 正人, 持田 讓冶
    • Journal Title

      臨床雑誌 整形外科 58

      Pages: 216-217

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioengineered chondrocyte sheets may be potentially useful for the treatment of partial thickness defects of articular cartilage2006

    • Author(s)
      Kaneshiro, N., Sato, M., Ishihara, M., Mitani, G., Sakai, H., Mochida, J
    • Journal Title

      Biochemical and Biophysical Research Communications 349(2)

      Pages: 723-731

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Bioengineered chondrocyte sheets may be potentially useful for the treatment of partial thickness defects of articular cartilage2006

    • Author(s)
      Nagatoshi Kaneshiro et al.
    • Journal Title

      Biochenical and Biophysical Research Communications 349

      Pages: 723-731

    • Related Report
      2006 Annual Research Report
  • [Presentation] 関節軟骨損傷治療のための軟骨細胞シート特性2007

    • Author(s)
      佐藤 正人, 他
    • Organizer
      第22回日本整形外科学会基礎学術集会
    • Place of Presentation
      浜松
    • Year and Date
      2007-10-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Potential of cell sheet engineering for treatment of partial thickness defect of articular cartilage2007

    • Author(s)
      Sato M, et. al.
    • Organizer
      Gordon Research Conference 2007
    • Place of Presentation
      Ventura,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The use of bioengineered chondrocyte sheets in treating partial thickness defects of articular cartilage2007

    • Author(s)
      Sato M, et. al.
    • Organizer
      53rd Annual Meeting of the Orthopaedic Research Society
    • Place of Presentation
      San Diego,California,USA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Technologies for articular cartilage regeneration2007

    • Author(s)
      Sato M, et. al.
    • Organizer
      SPIE Photonics West 2007
    • Place of Presentation
      San Jose,USA.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] [Invited paper] Technologies for articular cartilage regeneration2007

    • Author(s)
      Sato, M., Ishihara, M., Kaneshiro, N., Mitani, G., Nagai, T., Kutsuna, T., Kikuchi, M., Sakai, H., Mochida, J
    • Organizer
      SPIE Photonics West, San Jose
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The use of bioengineered chondrocyte sheets in treating partial thickness defects of articular cartilage2007

    • Author(s)
      Sato, M., Kaneshiro, N., Mitani, G., Nagai, T., Ishihara, M., Kutsuna, T., Sakai, H., Kikuchi, T
    • Organizer
      53rd Annual Meeting of the Orthopaedic Research Society San Diego, California
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] [Invited paper] Potential of cell sheet engineering for treatment of partial thickness defect of articular cartilage2007

    • Author(s)
      Sato, M., Mochida, J
    • Organizer
      Gordon Research Conference Cartilage Biology and Pathology, Ventura
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cell Sheet Technoogy for Cartilage Repair2006

    • Author(s)
      Sato M, et. al.
    • Organizer
      European Cells & Materials VII
    • Place of Presentation
      Davos,Switzerland
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cultured ArticularChondrocytes Sheets Using Temperature-Responsive Cultured Dishes2006

    • Author(s)
      Kaneshiro, N., Sato, M., Mitani, G., Nagai, T., Ishihara, M., Sakai, H., Mochida, J
    • Organizer
      6th International Cartilage Repair Society(ICRS) San Diego, California
    • Place of Presentation
      USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Potential of Cell Sheet Engineering for Treatment of Partial Thickness Defect of Articular Cartilage2006

    • Author(s)
      Sato, M., Kaneshiro, N., Mitani, G., Nagai, T., Ishihara, M., Sakai, H., Mochida, J
    • Organizer
      World Congress on Tissue Engineering and Regenerative Medicine
    • Place of Presentation
      Pittsburg USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] [Invited paper] Cell Sheet Technoogy for Cartilage Repair2006

    • Author(s)
      Masato, Sato and Joji, Mochida
    • Organizer
      European Cells & Materials VII Davos
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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