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Effect of interleukin-18 on metastasis of mouse osteosarcoma cells

Research Project

Project/Area Number 18591645
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionHyogo College of Medicine

Principal Investigator

FUTANI Hiroyuki  Hyogo College of Medicine, Faculty of Medicine, Associate professor (30248140)

Co-Investigator(Kenkyū-buntansha) TERADA Nobuyuki  Hyogo College of Medicine, Faculty of Medicine, Professor (50150339)
FUKUNAGA Satoru  Hyogo College of Medicine, Faculty of Medicine, Instructor (40441290)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsOsteosarcoma / Cytokine / Bone neoplasm / Interleukin / 骨肉種
Research Abstract

Osteosarcoma is the most common primary malignant bone tumor. Metastasis mostly in the lung invariably affects patient's survival. Therefore, the suppression of pulmonary metastasis is one of the most important issues in the treatment of osteosarcomas.
Interleukin-18 (IL-18), previously called interferon-γ (IFN-γ )-inducing factor, exerts an anti-tumor action through activation of natural killer cells (NK cells) or cytotoxic T lymphocytes, suppression of angiogenesis and induction of Fas-ligand on tumor cells expressing Fas. We have shown that IL-18 inhibits the growth of osteosarcoma cells by activating T lymphocytes, suggesting the usefulness of IL-18 as a therapy agent for osteosarcoma. In this study, we examined the effect of IL-18 on pulmonary metastasis of osteosarcoma using an experimental metastasis model in which highly metastatic LM8 mouse osteosarcoma cells were injected into a tail vein of T cell-deficient nude mice treated with anti-asialo GM1 serum to abolish the activity of NK cells. We found that IL-18 inhibited metastasis of osteosarcoma cells partly by inducing a factor(s) in the host which suppressed mobility of LM8 cells.
α-Galactosylceramide (α-GalCer) is known as antitumor agent by activating NK cells. We also found IL-18 in conjunction with α-GalCer exerts an antitumor effect on NK cell-sensitive tumors primarily by the direct stimulation of NK cells by IL-18 and the indirect stimulation of NK cells by α-GalCer through its activation of NKT cells.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (16 results)

All 2008 2007 2006 Other

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (4 results)

  • [Journal Article] Enhanced suppression of pulmonary metastasis of malignant melanoma cells by combined administration of alpha-galactosylceramide and interleukin-182008

    • Author(s)
      Nishio S.(他13名, 9番目)
    • Journal Title

      Cancer Science 99

      Pages: 113-20

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Enhanced suppression of pulmonary metastasis of malignant melanoma cells by combined administration of alpha-galactosylceramide and interleukin-182008

    • Author(s)
      Nishio S, et. al.
    • Journal Title

      Cancer Science 99

      Pages: 113-120

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Microgeodic disease affecting the toes in athletes : A report of 2 cases2008

    • Author(s)
      Kashiwa K, et. al.
    • Journal Title

      Am J Sports Med 36

      Pages: 1190-1192

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Enhanced suppression of pulmonary metastasis of malignant melanoma cells by combined administration of alpha-galactosylceramide and interleukin-182008

    • Author(s)
      Nishio S.(他13名、9番目)
    • Journal Title

      Cancer Science 99

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microgeodic disease affecting the toes in athletes:A report of 2 cases2008

    • Author(s)
      Kashiwa K.(他4名、3番目)
    • Journal Title

      Am J Sports Med

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Endoscopically assisted resection of a scapular osteochondroma causing snapping scapula syndrome2007

    • Author(s)
      Fukunaga S, et. al.
    • Journal Title

      World J Surg Oncol 5

      Pages: 37-37

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Successful treatment of bilateral calcaneal intraosseous lipomas using endoscopically assisted tumor resection2007

    • Author(s)
      Futani H, et. al.
    • Journal Title

      Anticancer Research 27

      Pages: 4311-4314

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Successful treatment of bilateral calcaneal intraosseous lipomas using endoscopically assisted tumor resection2007

    • Author(s)
      Futani H.(他4名、1番目)
    • Journal Title

      Anticancer Research 27

      Pages: 4311-4314

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of interleukin-18 on metastasis of mouse osteosarcoma cells2006

    • Author(s)
      Nakamura Y, (他9名, 7番目)
    • Journal Title

      Cancer Immunol Immunother 55

      Pages: 1151-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Long-term follow-up after limb salvage in skeletally immature children with a primary malignant tumor of the distal end of the femur2006

    • Author(s)
      Futani H, et. al.
    • Journal Title

      J Bone Joint Surg Am 88

      Pages: 595-603

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of interleukin-18 on metastasis of mouse osteosarcoma cells2006

    • Author(s)
      Nakamura Y, et. al.
    • Journal Title

      Cancer Immunol Immunother 55

      Pages: 1151-1158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Analyzing Knee Kinematics after Anterior Cruciate Ligament Reconstruction Using Fuzzy Logic2006

    • Author(s)
      D.Kudo, S.Kobashi, N.Shibanuma, A.Okayama, K.Kondo, M.Yagi, S.Yoshiya
    • Journal Title

      Int.Conf.on Soft Computing and Intelligent Systems 7

      Pages: 2007-2010

    • Related Report
      2006 Annual Research Report
  • [Presentation] Extracorporeal irradiated autogenous bone graft in limb-salvage of femur2007

    • Author(s)
      Nishio S.(他3名, 2番目)
    • Organizer
      14th International Symposium on Limb Salvage
    • Place of Presentation
      Hamburg,Germany
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Extracorporeal irradiated autogenous bone graft in limb-salvage of femur2007

    • Author(s)
      Nishio S, et. al.
    • Organizer
      14th International Symposium on Limb Salvage
    • Place of Presentation
      Hamburg Germany
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Successfully treated bilateral calcaneal intraosseous lipomas by endoscopically assisted tumor resection2007

    • Author(s)
      Futani H, et. al.
    • Organizer
      14th International Symposium on Limb Salvage
    • Place of Presentation
      Hamburg Germany
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Enhanced suppression of pulmonary metastasis of malignant melanoma cells by combined administration of alpha-galactosylceramide and interleukin-18

    • Author(s)
      Nishio S, et. al.
    • Organizer
      22nd Annual Research Meeting of the Japanese Orthopaedic Association
    • Place of Presentation
      Hamamatsu Shizuoka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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