Identification and characterization of a novel regulatory factor for the determination of osteoblast-adipocyte differentiation using RNA interference

• Project/Area Number: 18591656
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ARAI Yoshiyasu Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 講師 (10401390)
• Co-Investigator(Kenkyū-buntansha): 竹田 秀 東京医科歯科大学, 大学院・医歯学総合研究科, COE拠点形成特任教員 (30376727) ; 麻生 義則 東京医科歯科大学, 医学部附属病院, 助教 (50345279) ; 若林 良明 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (00431916)
• Co-Investigator(Renkei-kenkyūsha): TAKEDA Shu 慶應義塾大学, 医学部, 准教授 (30376727) ; ASOU Yoshinori 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (50345279) ; WAKABAYASHI Yoshiaki 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (00431916)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,100,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: RNA干渉 / 骨芽細胞 / 非翻訳RNA / 骨芽細胞分化 / スクリーニング / 脂肪細胞分化

Research Abstract

我々はタンパク質をコードしない小分子RNAに着目し、骨芽細胞分化における機能を検討した。その結果、mir-206が骨芽細胞分化を抑制すること、mir-206を過剰に発現するトランスジェニックマウスの骨量が低下することを明らかにし、マイクロRNAが骨芽細胞分化の生理的調節因子であることを世界で初めて報告した。

Research Products

• [Journal Article] A microRNA regulatory mechanism of osteoblast differentiation. (2009)
  • Author(s): Inose H, Ochi H, Kimura A, Fujita K, Xu R, Sato S, Iwasaki M, Sunamura S, Takeuchi Y, Fukumoto S, Saito K, Nakamura T, Siomi H, Ito H, Arai Y, Shinomiya K-i, Takeda S
  • Journal Title: Proceedings of the National Academy of Sciences 106 (49) Pages: 20794-20799
• [Journal Article] Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a critical role in chondroitin sulfate synthesis cartilage. (2007)
  • Author(s): Sakai K, Shinomiya K(6番目)計7人
  • Journal Title: J Biol Chem 282 Pages: 4152-61
  • Peer Reviewed
• [Journal Article] Central control of bone remodelling by Neuromedin U (2007)
  • Author(s): Sato S, Shinomiya K(16番目), Takeda S, ら(最終著者)計17人
  • Journal Title: Nat Med 13 Pages: 1234-40
  • Peer Reviewed
• [Journal Article] Prevention of cartilage destruction with intraarticular osteoclastogenesis inhibitory factor/osteoprotegerin in a marinemodel of osteoarthritis. (2007)
  • Author(s): Shimizu S, Asou Y(2番目), Shinomiya K(6番目)ら計7人
  • Journal Title: Arthritis Rheum 56 Pages: 3358-65
  • Peer Reviewed
• [Presentation] Regulation of osteoblast differentiation by microRNA ASBMR (2008)
  • Author(s): H. Inose, A. Kimura, M. Iwasaki, Y. Arai, K. Shinomiya, S. Takeda
  • Organizer: 30th Anuual Meeting
  • Place of Presentation: Motreal, Quebec, Canada
  • Year and Date: 2008-09-15
• [Presentation] Regulation of osteoblast differentiation by microRNA (2008)
  • Author(s): H. Inose, A. Kimura, M. Iwasaki, Y. Arai, K. Shinomiya, S. Takeda
  • Organizer: ASBMR 30th Annual Meeting
  • Place of Presentation: Montreal, Quebec, Canada
  • Year and Date: 2008-09-15
• [Presentation] Regulation of osteoblast differentiation by microRNA (2007)
  • Author(s): H. Inose, S. Takeda, S. Sato M. Iwasaki, A. Kimura and K. Shinomiya
  • Organizer: ASBMR 29th Annual Meeting
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2007-09-17
• [Presentation] Regulation of osteoblast differentiation by microRNA ASBMR (2007)
  • Author(s): H. Inose, S. Takeda, S. Sato, M. Iwasaki, A. Kimura, K.
  • Organizer: Shinomiya ASBMR annual meeting
  • Place of Presentation: Honolulu, Hawaii, USA