Study on full-thickness articular cartilage defect using concentration-gradient collage scaffold

• Project/Area Number: 18591660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Shiga University of Medical Science
• Principal Investigator: MATSUSUE Yoshitaka Shiga University of Medical Science, 医学部, 教授 (30209548)
• Co-Investigator(Kenkyū-buntansha): IMAI Shinji 滋賀医科大学, 医学部, 准教授 (90283556)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,080,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥780,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: 細胞動員 / 関節軟骨 / 軟骨細胞 / 3次元培養 / コラーゲン

Research Abstract

関節軟骨の全層欠損を修復するため用いられるコラーゲンの足場について家兎を用いて実験を行った。濃度勾配を有するラーゲンの足場が、骨髄由来の未分化間葉系細胞の総員能力の違いについて差があるか否かを検討するため、濃度勾配の異なる2種類のコラーゲンを作成し家兎の膝関節内の軟骨欠損部位に埋入した。結果として33%の濃度勾配を有するコラーゲンが最も細胞動員が良好で、組織学的なスコアも他のものに比べて優位に良好であった。

Research Products

• [Journal Article] A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair. (2008)
  • Author(s): Mimura T, Imai S, Kubo M, Isoya E, Ando K, Okumura N, Matsusue Y.
  • Journal Title: Osteoarthritis Cartilage. 16 Pages: 1083-1091
  • Peer Reviewed
• [Journal Article] Percutaneous transthoracic fenestration of an intramedullary neurenteric cyst in the thoracic spine with intraoperative magnetic resonance image navigation and thoracospy. (2008)
  • Author(s): Takahashi S, Morikawa S, Saruhashi Y, Matsusue Y, Kawakami M.
  • Journal Title: J Neurosurg Spine 9 Pages: 488-492
  • Peer Reviewed
• [Journal Article] A novel cxogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair (2008)
  • Author(s): Mimura T, Imai S, Kubo M, Isoya E, Ando K, Okumura N, Matsusue Y
  • Journal Title: Osteoarthritis Cartilage 16 Pages: 1083-1091
  • Peer Reviewed
• [Journal Article] Percutaneous transthoracic fenestration of an intramedullary neurenteric cyst in the thoracic spine with intraoperative magnetic resonance image navigation and thoracoscopy (2008)
  • Author(s): Takahashi S, Morikawa S, Saruhashi Y, Matsusue Y, Kawakami M
  • Journal Title: J Neurosurg Spine 9 Pages: 488-492
  • Peer Reviewed
• [Journal Article] The recovery of 5-HT transporter and 5-HT immunoreactivity in injured rat spinal cord (2008)
  • Author(s): Saruhashi Y, Matsusue Y, Fujimuya M
  • Journal Title: Arch Orthop Trauma Sure (Epub)
  • Peer Reviewed
• [Journal Article] Exogenous collagen-enhanced recruitment of mesenchymal stem cells during rabbit articular cartilage repair. (2007)
  • Author(s): Kubo M, Imai S, Fujimiya M, Isoya E, Ando K, Mimura T, Matsusue Y.
  • Journal Title: Acta Orthop 78 Pages: 845-855
  • Peer Reviewed
• [Journal Article] The MR tracking of transplanted ATDC5 cells using fluorinate poly-L-Lysine-CF3. (2007)
  • Author(s): Maki J, Masuda C, Morikawa S, Morita M, Inubushi T, Matsusue Y, Taguchi H, Tooyama I.
  • Journal Title: Biomaterials 28 Pages: 434-440
  • Peer Reviewed
• [Journal Article] Exogenous collag-enenhanced recruitment of mesenchymal stem cells during rabbit articular cartilage repair (2007)
  • Author(s): Kubo M, Imai S, Fujimiya M, Isoya E, Ando K, Mimura T, Matsusue Y
  • Journal Title: Acta Orthop 78 Pages: 845-855
  • Peer Reviewed
• [Journal Article] The MR tracking of transplanted ATDC5 cells using fluorinated poly-L-lysine-CF3 (2007)
  • Author(s): Maki J, Masuda C, Morikawa S Morita M, Inubushi T, Matsusue Y, Taguchi H, Tooyama I
  • Journal Title: Biomaterials 28 Pages: 434-440
  • Peer Reviewed
• [Journal Article] A 5-7 year in vivo study of high-strength hydroxyapatite/poly(L-lactide) composite rods for the internal fixation of bone fractures. (2006)
  • Author(s): Hasegawa S, Ishii S, Tamura J, Furukawa T, Neo M, Matsusue Y, Shikinami Y, Okuno M, Nakamura T.
  • Journal Title: Biomaterials 27 Pages: 1327-1332
  • Peer Reviewed
• [Journal Article] A 5-7 year in vivo study of high-strength hydroxyapatite/poly(L-lactide) composite rods for the internal fixation of bone fractures (2006)
  • Author(s): Hasegawa S, Ishii S, Tamura J, Furukawa T, Neo M, Matsusue Y, Shikinami Y, Okuno M, Nakamura T
  • Journal Title: Biomaterials 27 Pages: 1327-1332
• [Journal Article] Closed reduction for traumatic posterior dislocation of the shoulder using the 'lever principle' : two case reports and a review of the literature (2006)
  • Author(s): Mimura T, Mori K, Matsusue Y, Tanaka N, Nishi Y, Kobayashi M
  • Journal Title: Journal of Orthopaedic Surgery 14(3) Pages: 336-339
• [Presentation] Combination of collagen scaffold and timely release of bone morphogenetic protein-2 enhances the repair of full-thickness defect of articular cartilage. (2008)
  • Author(s): Mimura T, Imai S, Nishizawa K, Okumura N, Ando K, Kubo M, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] The role of the volume-sensitive Cl- current in the process of regulatory volume decrease(RVD) in freshly isolated articular chondrocytes. (2008)
  • Author(s): Okumura N, Toyota F, Isoya E, Imai S, Matsuura H, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] The effect of low-intensity pulsed ultrasound for formaitn of scaffold-free cartilage. (2008)
  • Author(s): Uenaka K, Imai S, Andou K, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] In-advance transmedullary stimulation of mesenchymal stem cells enhances spontaneous repair of full-thickness articular cartilage defect. (2008)
  • Author(s): Nishizawa K, Imai S, Mimura T, Kubo M, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] Mechanical stress-induced chronological phenotypic changes of chondrocytes in three-dimentional scaffold. (2008)
  • Author(s): Ando K, Imai S, Shioji S, Mimura T, Okumura N, Uenaka K, Kasahara T, Nishizawa K, Kubo M, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] Diabetes impairs fracture healing by repressing the osteoclast function. (2008)
  • Author(s): Kasahara T, Imai S, Matsusue Y, Fujimiya M, Kojima H.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] Mechanical stress modulates chondrocytes phenotype via IL-4. (2008)
  • Author(s): Shioji S, Imai S, Ando K, Mimura T, Okumura N, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008.
  • Place of Presentation: Rome, Italy.
  • Year and Date: 2008-09-19
• [Presentation] 膝の軟骨損傷に対する治療戦略 (2008)
  • Author(s): 松末吉隆
  • Organizer: 第9回北海道スポーツ医学フォーラム
  • Place of Presentation: 札幌市
  • Year and Date: 2008-07-19
• [Presentation] Effect of dynamic compressive loading in combination with growth factor on the cartilage-specific gene expression of 3D scaffold-embedded chondrocytes. (2007)
  • Author(s): Ando K, Imai S, Isoya E, Kubo M, Okumura N, Mimura T, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2007.
  • Place of Presentation: Fort Lauderdale, Florida
  • Year and Date: 2007-12-07
• [Presentation] A novel concentration-gradient collagen scaffold augments due. (2007)
  • Author(s): Mimura T, Imai S, Kubo M, Isoya E, Okumura N, Ando K, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2007.
  • Place of Presentation: Fort Lauderdale, Florida
  • Year and Date: 2007-12-07
• [Presentation] Repair of joint surface by autogenous osteochondral graft transplantation for steroid-induced osteonecrosis of the knee. (2006)
  • Author(s): Matsusue Y, Kubo M, Kikkawa M, Nakagawa Y.
  • Organizer: International Cartilage Repair Society 6th Symposium.
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2006-01-08
• [Presentation] Swelling-activated chloride current and its inhibition by arachidonic acid in rabbit articular chondrocytes. (2006)
  • Author(s): Isoya E, Matsuura H, Toyoda F, Imai S, Kubo M, Matsusue Y.
  • Organizer: International Cartilage Repair Society 6th Symposium.
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2006-01-08
• [Presentation] Collagen scaffold augments large full-thickness articular cartilage repair by active mesenchymal cell recruitment. -detaild cellular events during cartilage repair-. (2006)
  • Author(s): Kubo M, Imai S, Isoya E, Ando K, Matsusue Y.
  • Organizer: International Cartilage Repair Society 6th Symposium.
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2006-01-08
• [Book] 関節軟骨:最新整形関節軟骨:最新整形外科学大系1.運動器の生物学と生体力学 (2008)
  • Author(s): 今井晋二、松末吉隆
  • Publisher: 中山書店(越智隆弘監修)
• [Book] 関節軟骨 : 最新整形外科学大系(越智隆弘監修) (2008)
  • Author(s): 今井晋二, 松末吉隆
  • Total Pages: 6
  • Publisher: 中山書店