| Project/Area Number |
18591678
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
SATO Kazuki Keio University, Department of Orthopedic Surgery, Assistant (60235322)
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| Co-Investigator(Kenkyū-buntansha) |
ISSHIKI Honoka Keio University, Department of Orthopedic, Assistant (00348674)
UMEZAWA Akihiro KEIO UNIVERSITY, Department of Reproductive Biology, National Research Institute for Child Health and Development, Director (70213486)
TOYAMA Yoshiaki Keio University, Department of Orthopedic Surgery, Professor (40129549)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,920,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | epiphyseal plate / gene expression / mouse |
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| Research Abstract |
Longitudinal bone growth is a complex process that begins chondrocyte proliferation and subsequent hypertrophy and secretion of extracellular matrix. Subsequently matrix mineralization, vascular invasion, chondrocyte apoptosis, and replacement of chondrocytes by osteocytes lead to the formation of bone. The chondrocytes of the growth plate have been classified into four anatomic zones, reflecting their cellular features and the tissue architecture. In the resting zone (RZ),the ratio of extracellular matrix to cell volume is quite high. The chondrocytes take on a flattened appearance, begin to divide, and organize into columns that extend from proliferating zone (PZ) to maturing zone (MZ). The dividing cells separate from each other and grow larger in the hypertrophic zone (HZ).
There are notable biochemical and morphological differences among chondrocytes in each zone. In order to better characterize the genetic program controlling bone growth, gene expression was analyzed in chondrocytes microdissected from four previously defined zones of the growth plate. This study is the first to examine the growth plate at such resolution and further extends previous work by measuring expression of ~34,000 putative transcripts. We identified significant differences in gene expression among zones in growth plate, which likely contribute to the regulation of chondrocyte maturation in the growth plate.
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