Role of HMGB-1 in septic encephalopathy during sepsis

• Project/Area Number: 18591688
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Gunma University
• Principal Investigator: KADOI Yuji Gunma University, 医学部, 准教授 (10292591)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,880,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 敗血症 / アポトーシス / 脳障害 / HMGB-1 / 海馬 / 電気泳動 / 脳症 / エンドトキシン

Research Abstract

盲腸穿孔モデルによる実験的敗血症性中枢神経障害を作成し、脳神経細胞のアポトーシスの発現部位をTUNEL染色法を用いて、HMGB-1の発現部位については抗体法を用いて観察した。その結果、脳内においても海馬部位のCA1とCA5により多くのアポトーシスが発現しておりかつ同部位にHMGB-1も多く発現していることが判明した。以上のことからHMGB-1とアポトーシスとの関連を見いだし、抗HMGB-1抗体を投与することで改善可能かを検討した実験では中枢神経障害が改善し、HMGB-1が中枢神経障害に関与することを解明できた。

Research Products

• [Journal Article] L-Arginine decreases fluid-percussion injury-induced neuronal nitrotyrosineimmunoreactivity in rats (2008)
  • Author(s): Avila MA, Sell SL, Kadoi Y, Prough DS, Hellmich HL, Velasco M, Dewitt DS
  • Journal Title: J Cereb Blood Flow Metab 28 Pages: 1733-1741
  • Peer Reviewed
• [Journal Article] Evaluation of cerebrovascular carbon dioxide reactivity in patients with diabetes mellitus under sedative doses of propofol (2008)
  • Author(s): Kawauchi C, Kadoi Y, Hinohara H, Fumio Kunimoto K, Saito S
  • Journal Title: J Anesthesia 22 Pages: 429-434
  • NAID: 10024460901
  • Peer Reviewed
• [Journal Article] The comparative effects of sevoflurane versus isoflurane on cerebrovascular carbon dioxide reactivity in patients with previous stroke (2008)
  • Author(s): Kadoi Y, Saito S, Takahashi K
  • Journal Title: J Anesthesia 22 Pages: 135-139
  • NAID: 10024128979
  • Peer Reviewed
• [Journal Article] Comparative effects of propofol, landiolol and nicardipine on hemodynamic and bispectral index responses to endotracheal intubation : Prospective randomized double-blinded study (2008)
  • Author(s): Miyazaki M, Kadoi Y, Sudo T, Sawano Y, Shimada H
  • Journal Title: J Clinical Anesth 20 Pages: 257-262
  • Peer Reviewed
• [Journal Article] Effects of the cannabinoid antagonist AM281 on systemic hemodynamics and mortality rate in streptozotocin- induced diabetic rats with endotoxic shock-Comparison between non-diabetic and diabetic rats (2008)
  • Author(s): Kadoi Y, Hinohara H, Kunimoto F, Saito S
  • Journal Title: Acta Anaesthesiol. Scand 52 Pages: 664-672
  • Peer Reviewed
• [Journal Article] Comparative effects of propofol versus dexmedetomidine on cerebrovascular carbon dioxide reactivity in patients with septic shock (2008)
  • Author(s): Kadoi Y, Saito S, Kawauchi C, Hinohara H, Kunimoto F
  • Journal Title: Br. J Anesth 100 Pages: 224-229
  • Peer Reviewed
• [Journal Article] The comparative effects of sevoflurane versus isoflurane on cerebrovascular carbon dioxide reactivity in patients with previous stroke. (2008)
  • Author(s): Kadoi Y, Saito S, Takahashik.
  • Journal Title: Journal of Anesthesia 22 Pages: 135-139
  • NAID: 10024128979
  • Peer Reviewed
• [Journal Article] Effects of the cannabinoid antagonist AM281 on systemic hemodynamics and mortality rate in streptozotocin- induced diabetic rats with endotoxic shock- Comparison between non-diabetic and diabetic rats (2008)
  • Author(s): Kadoi Y, Hinohara H, Kunimoto F, Saito S
  • Journal Title: Acta Anaesthesiolica Scandinavica 52 Pages: 664-672
  • Peer Reviewed
• [Journal Article] Comparative effects of propofol versus dexmedetomidine on cerebrovascular carbon dioxide reactivity in patients with septic shock (2008)
  • Author(s): Kadoi Y, Saito S, Kawauchi C, Hinohara H. Kunimoto F
  • Journal Title: British Journal of anesthesia 100 Pages: 224-229
  • Peer Reviewed
• [Journal Article] The comparative effects of sevoflurane versus isoflurane on cerebrovascular carbon dioxide reactivity in patients with hypertension. Acta Anaesthesiol (2007)
  • Author(s): Kadoi Y, Saito S, Takahashi K
  • Journal Title: Scand 51 Pages: 1382-1387
  • Peer Reviewed
• [Journal Article] Sevoflurane anesthesia did not affect postoperative cognitive dysfunction in patients undergoing coronary artery bypass graft surgery (2007)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: J Anesthesia 31 Pages: 30-335
  • NAID: 10019811859
  • Peer Reviewed
• [Journal Article] Effects of selective inducible nitric oxide synthase inhibition on systemic hemodynamics and mortality rate on endotoxic shock in streptozotocin- induced diabetic rats (2007)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: Shock 28 Pages: 602-610
  • Peer Reviewed
• [Journal Article] Effects of nicardipine-induced hypotension on cerebrovascular carbon dioxide reactivity in patients with diabetes mellitus under sevoflurane anesthesia (2007)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: J Anesthesia 27 Pages: 125-130
  • NAID: 10020093358
  • Peer Reviewed
• [Journal Article] Effects of selective inducible nitric oxide synthase inhibition on systemic hemodynamics and mortality rate on endotoxic shock in streptozotocin-induced diabetic rats (2007)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: Shock 24 Pages: 602-610
  • Peer Reviewed
• [Journal Article] Effects of nicardipine-induced hypotension on cerebrovascular carbon dioxide reactivity in patients with diabetes mellitus under sevoflurane anesthesia (2007)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: J Anesthesia 21 Pages: 125-130
  • NAID: 10020093358
  • Peer Reviewed
• [Journal Article] Factors associated with postoperative cognitive dysfunction in patients with cardiac surgery (2006)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: Surgery Today 36 Pages: 1053-1057
  • Peer Reviewed
• [Journal Article] Effects of AM 281, a cannabinoid antagonist, on circulatory deterioration and cytokine production in endotoxin shock model-Comparison with norepinephrine (2006)
  • Author(s): Kadoi Y, Goto F
  • Journal Title: J Anesthesia 20 Pages: 284-289
  • NAID: 10019270085
  • Peer Reviewed
• [Journal Article] Effects of antihypertensive medication on left ventricular function during electroconvulsive therapy : study by transthoracic echocardiography (2006)
  • Author(s): Kadoi Y, Takahashi K, Saito S, Goto F
  • Journal Title: J Clinical Anesth 18 Pages: 441-445
  • Peer Reviewed
• [Journal Article] Comparative effects of sevoflurane versusisoflurane on cerebrovascular carbon dioxide reactivity in patients with diabetes mellitus (2006)
  • Author(s): Kadoi Y, Takahashi K, Saito S, Goto F
  • Journal Title: Anesth. Analg 103 Pages: 168-172
  • Peer Reviewed
• [Journal Article] Factors associated with postoperative cognitive dysfunction in patients with cardiac surgery (2006)
  • Author(s): Kadoi Y
  • Journal Title: Surgery Today 36 Pages: 1053-1057
• [Journal Article] Effects of AM 281, a cannabinoid antagonist, on circulatory determination and cytokine production in endotoxin shock model-Comparison with norepinephrine. (2006)
  • Author(s): Kadoi Y
  • Journal Title: J Anesthesia 20 Pages: 284-289
• [Journal Article] Effects of antihypertensive medication on left ventricular function during electroconvulsive therapy : study by transhoratic echocardiography. (2006)
  • Author(s): Kadoi Y
  • Journal Title: J Clinical Anest 18 Pages: 441-445
• [Journal Article] Comparative effects of sevoflurane versus isoflurane on cerebrovascular carbon dioxide reactivity in patients with diabetes mellitus. (2006)
  • Author(s): Kadoi Y
  • Journal Title: Anesh. Analg 103 Pages: 168-172
• [Presentation] 中心静脈血液ガス測定のすすめ (2009)
  • Author(s): 日野原宏,三田範勝,大川牧生,大嶋清宏,門井雄司,斎藤繁,国元文生
  • Organizer: 第36回日本集中治療医学会
  • Place of Presentation: 大阪
• [Presentation] 気管挿管時の血行動態変動に対する各種薬物投与の効果の比較検討 (2008)
  • Author(s): 須藤 貴史、宮崎増美、澤野由加梨、嶋田均、門井雄司、齋藤繁
  • Organizer: 第55回日本麻酔科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-03
• [Presentation] ARDS患者に対するNO吸入テストは腹臥位療法の有効性を予測可能か (2007)
  • Author(s): 門井雄司、後藤文夫
  • Organizer: 第22回日本ショック学会総会
  • Place of Presentation: 京都
  • Year and Date: 2007-05-30
• [Book] 緊急麻酔の心得と知識、第7章「腹部外科手術の緊急麻酔」 (2008)
  • Author(s): 門井雄司、国元文生
  • Publisher: 克誠堂(株)