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Effects of the P/Q-type calcium channel of ischemic neuronal damage characterized by neuronal functional images

Research Project

Project/Area Number 18591692
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNiigata University

Principal Investigator

FUJIWARA Naoshi  Niigata University, Institution of Medicine and Dentistry, Professor (70181419)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,160,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordscerebral cortex / hippocampus / voltage-sensitive dye / intracellular calcium / P / Q-type calcium channel / ω-agatoxin IVA / GABA_A receptor / thiamylal / ω-アガトキシン / チアミラール
Research Abstract

This study aims to clarify the relationship between the P/Q-type calcium channel or the GABAA receptor and ischemic neuronal damage. Effects of a P/Q-type calcium channel blocker and GABAA receptor modulator on ischemic neuronal damage were investigated by means of spatio-temporal analyses of excitation propagation and intracellular calcium changes in cortical and hippocampal slices of the mouse brain.
(1) Electrical stimulation to layer V of a cortical slice evoked transient depolarization in the vicinity of stimulated site. Then this excitatory response propagated to layers II-III and widely expanded in these layers. Intracellular calcium level was also elevated in layers II-III. The excitation propagation and calcium elevation were inhibited by a P/Q-type calcium channel blocker ω-agatoxin IVA(50 nmol/l). These results suggest that the P/Q-type calcium channel plays an important role in excitation propagation in the cerebral cortex.
(2) Excitation propagation in both cortical and hippocampal slices was preserved in an oxygenated Krebs solution over 20 hours after their preparation. But, in slices exposed to transient in vitro ischemia (oxygen-glucose deprivation from a bathing medium) for 5 min, excitation propagation almost disappeared 18-20 hours after the ischemic exposure. This neuronal dysfunction also occurred in the slices exposed to In vitro ischemia in the presence of w-agatoxin IVA (200 nmol/l), suggesting that the neuronal dysfunction cannot be prevented by blocking the P/Q-type calcium channel.
(3) A GABA_A receptor modulator thiamylal (200 μmol/l) inhibited the excitation propagation in cortical slices. Excitation propagation was partially preserved in both cortical and hippocampal slices after the exposure to transient in vitro ischemia in the presence of thiamylal. The result suggests that the enhancement of GABA_A receptor activity protects the ischemic neuronal dysfunction in the cortex and hippocampus.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006 Other

All Journal Article (8 results) (of which Peer Reviewed: 1 results) Presentation (10 results) Remarks (2 results)

  • [Journal Article] マウス大脳皮質と海馬の局所興奮伝搬に及ぼすサイアミラールの作用2008

    • Author(s)
      藤原直士
    • Journal Title

      神経麻酔・集中治療 11

      Pages: 45-47

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Journal Article] Effects of thiamylal on regional excitation propagation in cerebral cortex and hippocampus of the mouse.(in Japanese)2008

    • Author(s)
      N. Fujiwara
    • Journal Title

      Neuroanesthesia and Critical Care 11

      Pages: 45-47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Iteration of high-frequency stimulation enhances long-lasting excitatory responses in the spinal dorsal horn of rats: characterization by optical imaging of signal propagation2007

    • Author(s)
      M. Ogawa
    • Journal Title

      Neuroscience Research 57・3

      Pages: 467-472

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] イントロダクション:麻酔科医が求める麻酔メカニズム研究のあり方について2007

    • Author(s)
      馬場 洋
    • Journal Title

      麻 酔 56増刊

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Iteration of high-frequency stimulation enhances long-lasting excitatory responses in the spinal dorsal horn of rats: characterization by optical imaging of signal propagation2007

    • Author(s)
      M. Ogawa
    • Journal Title

      Neuroscience Research 57(3)

      Pages: 467-472

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Introduction: researches on anesthetic mechanism by anesthesiologists.(in Japanese)2007

    • Author(s)
      H. Baba
    • Journal Title

      Masui 56(Supplement)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] イントロダクション:麻酔科医が求める麻酔メカニズム研究のあり方について2007

    • Author(s)
      馬場 洋
    • Journal Title

      麻酔 56増刊

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Iteration of High-frequency Stimulation Enhances Long-lasting Excitatory Responses in the Spinal Dorsal Hom of Rats : Characterization by Optical Imaging of Signal Propagation2007

    • Author(s)
      M.Ogawa
    • Journal Title

      Neuroscience Research 57・3

      Pages: 467-472

    • Related Report
      2006 Annual Research Report
  • [Presentation] Inhibitory effects of thiamylal on excitation propagation in brain slices2007

    • Author(s)
      藤原直士
    • Organizer
      第30回日本神経科学会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Inhibitory effects of thiamylal on excitation propagation in brain slices2007

    • Author(s)
      N, Fujiwara
    • Organizer
      The 30th Annual Meeting of The Japan Neuroscience Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 脳切片の興奮伝搬に及ぼすサイアミラールの抑制作用2007

    • Author(s)
      藤原 直士
    • Organizer
      第30回日本神経科学大会
    • Place of Presentation
      横浜市
    • Year and Date
      2007-09-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] サイアミラールによる中枢神経興奮抑制の画像解析2007

    • Author(s)
      小川真有美
    • Organizer
      第54回日本麻酔学会総会
    • Place of Presentation
      札幌
    • Year and Date
      2007-06-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Spatio-temporal analyses of inhibitory effects of thiamylal on excitation of central nervous system.(in Japanese)2007

    • Author(s)
      M, Ogawa
    • Organizer
      The 54th Annual Meeting of Japan Society of Anesthesiology
    • Place of Presentation
      Sapporo
    • Year and Date
      2007-06-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス大脳皮質と海馬の局所興奮伝搬に及ぼすサイアミラールの作用2007

    • Author(s)
      藤原直士
    • Organizer
      第11回日本神経麻酔・集中治療研究会
    • Place of Presentation
      秋田
    • Year and Date
      2007-04-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of thiamylal on regional excitation propagation in cerebral cortex and hippocampus of the mouse.(in Japanese)2007

    • Author(s)
      N, Fujiwara
    • Organizer
      The 11th Annual Meeting of Japan Society of Neuroanesthesia and Critical Care
    • Place of Presentation
      Akita
    • Year and Date
      2007-04-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] マウス大脳皮質と海馬の局所興奮伝搬に及ぼすサイアミラールの作用2007

    • Author(s)
      藤原 直士
    • Organizer
      日本神経麻酔・集中治療研究会
    • Place of Presentation
      秋田市
    • Year and Date
      2007-04-21
    • Related Report
      2007 Annual Research Report
  • [Presentation] Thiamylal may more effectively reduce neuronal excitability in cerebral cortex than that in hippocampus2006

    • Author(s)
      藤原直士
    • Organizer
      第29回日本神経科学会学術大会
    • Place of Presentation
      京都
    • Year and Date
      2006-07-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Thiamylal may more effectively reduce neuronal excitability in cerebral cortex than that in hippocampus2006

    • Author(s)
      N, Fujiwara
    • Organizer
      The 29th Annual Meeting of The Japan Neuroscience Society
    • Place of Presentation
      Kyoto
    • Year and Date
      2006-07-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.clg.niigata-u.ac.jp/~naochan/fuji_top/CFA/index.htm

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.clg.niigata-u.ac.jp/~naochan/fuji_top/CFA/index.htm

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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