| Project/Area Number |
18591710
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Oita University |
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Principal Investigator |
NOGUCHI Takayuki Oita University, faculty of medicine, Anesthesiology, professor (90156183)
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| Co-Investigator(Kenkyū-buntansha) |
KIRA Shinichirou Oita University, faculty of medicine, Anesthesiology, Research Associate (70404374)
MORI Masakazu Oita University, faculty of medicine, Anesthesiology, assistant professor (20220022)
MIZUTANI Akio Oita University, faculty of medicine, Anesthesiology, assistant professor (20260739)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | acute lung injury / hyperthe mia / hypothermia / inflamation / cytokine / ハイポサーミア / ハイパーサーミア / NF-kB / IL-1β / IL-6 / TNF-α / HSP / HSF |
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| Research Abstract |
To examine whether moderate changes in cell culture temperature influence the production of various cytokines and associated mediators of inflammation. We performed lipopolysaccharide (LPS) stimulation of the murine macrophagic RAW264.7 cell line under hyperthermic (40℃), normothermic (37℃) and hypothermic (34℃) conditions. We then measured the levels of heat shock protein 70 (HSP70), heat shock factor protein (HSF) and nuclear factor-kB (NF-kB) dimers (p50 and p65)in the cells, and the levels of high mobility group box 1 (HMGB1) and the cytokines tumor necrosis fector-oc (TNF-a), interleukin ip (IL-ip) and interleukin 6 (IL-6) n the culture supernatants. Levels of HMGB1, IL-ip, IL-6,and TNF-a, as well as NF-kB dimers (p50 and p65), were all reduced following LPS stimulation at 40℃ and 34℃ compared with those at 37℃. Levels of HSP70 and HSF increased at 40℃ and 34℃. The application of moderate hyperthermia and hypothermia after LPS-induced cell activation attenuated the inflammatory response and reduced the likelihood of cell damage. These findings suggest that moderate temperature changes modulate the inflammatory response and could be a useful therapy against sepsis.
These findings are available to treat inflammation of ventilator induced lung injury.
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