A research of new cerebroprotection method discovered from cross talk among intracerebral compartments
| Project/Area Number |
18591725
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | Tokyo Medical University |
|---|
Principal Investigator |
MUROZONO Michihiro Tokyo Medical University, School of Medicine, Assistant Professor (70276947)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Shohei Tokyo Medial University, School of Medicine, Assistant Professor (30256250)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,190,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Brain ischemia / P-glycoprotein / blood-brain barrier |
|---|
| Research Abstract |
We measured apoptotic factors, cytokines and factors for tissue repair for the evluation of postischemic influence of P-gp by using immunohistochemistry, western blotting, and ELISA this time. In addition ; we investigated how mdrla P-gp existing in brain of normal mice changed compared with that of mdrla knockout mice. In mdrla knockout mice, Bcl-2 was more and bax was less after ischemia than those of wild-type mice significantly.
Therefore, antiapoptosis action strongly worked on the mdrla ko mouse in comparison with wild-type mice on brain ischemia. After transient cerebral ischemia, on wild-type mice p-gp expressed contineously and without decreaseing, so it is possible that p-gp can act as apotosis inducer at the early stage on brain ischemia.
On the other hand, by a combined medication of cyclosporiti A and Ondansetron, damage of cerebral ischemia decreased significantly. From the above, it is suggested that when it uses cerebroprotection agent which depends on P-gp for treatment for cerebral ischemia, it does a cerebroprotection effect effectively to use other materials dependent on P-gp together.
|
Report
(3 results)
Research Products
(7 results)
