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Transplantation of preserved human amniotic membrane for bladder augmentation and urethral sub-mucosal injection

Research Project

Project/Area Number 18591744
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionShinshu University

Principal Investigator

SEKI Satoshi  Shinshu University, Department of Clinical Urologic and Pharmacological Researcb, Associate Professor (60293502)

Co-Investigator(Kenkyū-buntansha) IGAWA Yashuhiko  Shinshu University, Department of Urology, Associate Professor (40159588)
ISHIZUKA Osamu  Shinshu University, Department of Urology, Associate Professor (20184541)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,830,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsamniotic membren / bladder augmentation / urinary incontinence / tissue engineering / voiding dysfunction / 羊膜 / 萎縮膀胱
Research Abstract

Gastrointestinal segments have been widely used for bladder augmentation. However, they are not ideal because of the possible complications. Firstly, we performed bladder augmentation in rats using human amniotic membrane (hAM). Morphologically, the hAM-augmented bladder exhibited regeneration of urothelium, detrusor smooth muscle, and nerve fibers within 3 months post-operatively.
By functional evaluation of the bladder strips, we compared hAM-augmented bladders with bladders augmented by small intestinal submucosa (SIS). Both hAM- and SIS-augmented bladders displayed adequate capacity and compliance. The results indicate that, for bladder augmentation, hAM can be used as a scaffold and is comparable in this respect with SIS.
Secondly, we confirmed that crashed and cultured amniotic cells injected under bladder mucosa exhibited differentiation into smooth muscle cells using immunohistochemical studies.
Human amniotic membrane can be more easily obtained than other materials and requires little preparation, and its use raises few ethical questions. Therefore, hAM may represent a new therapeutic alternative for urological reconstructions.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (8 results)

All 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (1 results)

  • [Journal Article] Transplantation of Preserved Human Amniotic Membrane for Bladder Augmentation in Rats2007

    • Author(s)
      Kazuyoshi Iijima
    • Journal Title

      Tissue Engineering 12

      Pages: 513-524

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Botulinum A Toxin Treatment for Idiopathic Detrusor Overactivity2007

    • Author(s)
      Seki S.
    • Journal Title

      Incont Pelvic Floor Dysfunct 1

      Pages: 5-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of IP-751, Ajulemic Acid, on Bladder Overactivity Induced by Bladder Irritation in Rats2007

    • Author(s)
      Hiragata S
    • Journal Title

      Urology 70(1)

      Pages: 202-205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Transplantation of Preserved Human Amniotic Membrane for Bladder Augmentation in Rats2007

    • Author(s)
      Kazuyoshi, Iijima
    • Journal Title

      Tissue Engineering Vol.12

      Pages: 513-524

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Transplantation of Preserved Human Amniotic Membrane for Bladder Augmentation in Rats2007

    • Author(s)
      Kazuyoshi Iijima
    • Journal Title

      Tissue Engineering 13

      Pages: 513-524

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bladder Overactivity and Hyperexcitability of Bladder Afferent Neurons after Intrathecal Delivery of Nerve Growth Factor in Rats2006

    • Author(s)
      Yoshimura N
    • Journal Title

      J Neurosci 26(42)

      Pages: 10847-10855

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Dopaminergic mechanisms controlling urethral function in rats2006

    • Author(s)
      Ogawa Y
    • Journal Title

      Neurourol Urodyn 25(5)

      Pages: 480-489

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Presentation] ヒト羊膜由来間葉系細胞のラット膀胱粘膜下移植2007

    • Author(s)
      皆川 倫範
    • Organizer
      第4回泌尿器科再建再生研究会
    • Place of Presentation
      高松市
    • Year and Date
      2007-06-16
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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