Research on non-familial kidney cancer receptivity gene by SNP analysis

• Project/Area Number: 18591758
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Kochi University
• Principal Investigator: AKIMARU Kunihiro (2007) Kochi University, Kcchi Medical School, Environmental Medicine, Assistant Professor (50281184); 山崎 一郎 (2006) 高知大学, 医学部附属病院, 助手 (40315015)
• Co-Investigator(Kenkyū-buntansha): SHUIN Taro Kochi University, Kochi Medical School, Urology, Professor (80179019) ; MASAYUKI Kamata Kochi University, Kochi Medical School Hospital, Urology, Associate Professor (90304683) ; 秋丸 国広 高知大学, 医学部, 助手 (50281184) ; 中村 裕之 高知大学, 医学部, 教授 (30231476)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,980,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥480,000); Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
• Keywords: renal cell carcinoma / tumor marker / drug transporter / gene Delmore hysm / case-control study / 腎癌 / SNP / トランスポーター

Research Abstract

The gene analysis for receptive gene candidate and gene expression analysis were intended to establish the new treatment method and the preventive of the renal cell carcinoma (1) Genome DNA for the research was a case who accumulated with the urinary organs disease genome society that consisted of 11 nationwide universities, and they were 241 sample of example DNA of the renal tumor registration syndrome examples and 186 contrast group examples. Genetic polymorphism was analyzed about three SNPs of OCT1 and three SNPs of OAT1, and the SNP frequency was compared. A big difference turned out SNP frequency information in a public SNP data base though a significant difference was not admitted in any sere in the patient group and the control group. The reflection of regional uniqueness of the object groups, or the difference of an analytical population and the analysis method were thought for this, and it was felt that it was necessary to advance the analysis more in detail. (2) There candi date gene expression analysis in the renal adenocarcinoma section obtained from patients by nephrectomy showed that these protein egression were extremely low in tumor region. (3) The influence of receptive SNP on the transporter function was analyzed by gene expression analysis in renal cell cultures. The stationary state gene expression, and also gene induction/suppression by cell proliferation promotor or inflammation causing chemicals were measured, and compared. Moreover, the effect of sex-related hormone on gene induction/suppression was compared, because gender difference of transporter egression level in the rat is known. The gene expression level was analyzed in correlation with the genotype of the object renal carcinoma cell culture. The expression level of OCT1 or OAT1 was a little different according to the cells, however it was extremely low and induction/suppression with tested drugs were different, there was no correlation to SNP genotype. As same as analysis fir transporter's expression in tumor region, it seemed that a more detailed examination was necessary. The new renal carcinoma marker's development and the retrieval of the new treatment target were expected by further promotion of this research for the examples for the further genome analysis to be accumulating from nationwide multi facilities.

Research Products

• [Journal Article] Blockade of the vascular endothelial growth factor-receptor 2 pathway inhibits the growth of human renal cell carcinoma, RBM1-IT4, in the kidney but not in the bone of nude mice (2007)
  • Author(s): Karashima T, Inoue K, Fukata S, Iiyama T, Kurabayashi A, Kawada C, Shuin T.
  • Journal Title: Int J Oncol. 30 Pages: 937-945
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Renal leiomyoma: an inlmunohistochemical,ultrastructural and comparative genomic hybridization study (2007)
  • Author(s): Kuroda N, Inoue Y, Taguchi T, Tominaga A, Hes O, Michal M, Hayashi Y, Hiroi M, Shuin T, Lee GH.
  • Journal Title: Histol Histopathol. 22 Pages: 883-888
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Modification in the von Hippel-Lindau protein is involved in the progression of experimentally induced rat glomerulonephritis (2007)
  • Author(s): Kudo Y, Kakinuma Y, Iguchi M, Sato T, Sugiura T, Furihata M, Shuin T.
  • Journal Title: Nephron Exp Nephrol. 106
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] WT1(Wilms' tumor 1)peptide immunotherapy for renal cell carcinoma (2007)
  • Author(s): Iiyama T, Udaka K, Takeda S, Takeuchi T, Adachi YC, Ohtsuki Y, Tsuboi A, Nakatsuka S, Elisseeva OA, Oji Y, Kawakami M, Nakajima H, Nishida S, Shirakata T, Oka Y, Shuin T, Sugiyama H.
  • Journal Title: Microbiol Immunol. 51 Pages: 519-530
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ethanol consumption alters expression and colocalization of bile salt export pump and multidrug resistance protein 2 in the rat. (2007)
  • Author(s): Zinchuk V, Zinchuk O, Akimaru K, Moriya F, Okada T.
  • Journal Title: Histochem Cell Biol 127 Pages: 503-512
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Blockade of the vascular endothelial growth factor-receptor 2 pathway inhibits the growth of human renal cell carcinoma, RBM1-IT4, in the kidney but not in the bone of nude mice (2007)
  • Author(s): Karashima, T, Inoue, K, Fukata, S, Iiyama, T, Kurabayashi, A, Kawada, C, Shuin, T
  • Journal Title: Int J Oncol 30 Pages: 937-945
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] an immunohistochemical, ultrastructural and comparative genomic hybridization study (2007)
  • Author(s): Kuroda, N, Inoue, Y, Taguchi, T, Tominaga, A, Hes, O, Michal, M, Hayashi, Y, Hiroi, M, Shuin, T, Lee, GH, Renal, leiomyoma
  • Journal Title: Histol Histopathol 22 Pages: 883-888
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Modification in the von Hippel-Lindau protein is involved in the progression of experimentally induced rat glomerulonephritis (2007)
  • Author(s): Kudo, Y, Kakinuma, Y, Iguchi, M, Sato, T, Sugiura, T, Furihata, M, Shuin, T
  • Journal Title: Nephron Exp Nephrol 106 Pages: 97-106
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] 「研究成果報告書概要(欧文)」より (2007)
  • Author(s): Iiyama, T, Udaka, K, Takeda, S, Takeuchi, T, Adachi, YC, Ohtsuki, Y, Tsuboi, A, Nakatsuka, S, Elisseeva, OA, Oji, Y, Kawakami, M, Nakajima, H, Nishida, S, Shirakata, T, Oka, Y, Shuin, T, Sugiyama, H
  • Journal Title: Microbiol Immunol 51 Pages: 519-530
• [Journal Article] Ethanol consumption alters expression and colocalization of bile salt export pump and multidrug resistance protein 2 in the rat (2007)
  • Author(s): Zinchuk, V, Zinchuk, O, Akimaru, K, Moriya, F, Okada, T
  • Journal Title: Histochem Cell Biol 127 Pages: 503-512
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Blockade of the vascular endothelial growth factor-receptor 2 pathway inhibits the growth of human renal cell carcinoma, RBM1-IT4, in the kidney but not in the bone of nude mice (2007)
  • Author(s): Karashima T, Inoue K, Fukata S, Iiyama T, Kurabayashi A, Kawada C, Shuin T.
  • Journal Title: Int J Oncol 30 Pages: 937-945
  • Peer Reviewed
• [Journal Article] Renal leiomyoma:an immunohistochemical, ultrastructural and comparative genomic hybridization study (2007)
  • Author(s): Kuroda N, Inoue Y, Taguchi T, Tominaga A, Hes O, Michal M, Hayashi Y, Hiroi M, Shuin T, Lee GH.
  • Journal Title: Histol Histopathol 22 Pages: 883-888
  • Peer Reviewed
• [Journal Article] Modification in the von Hippel-Lindau protein is involved in the progression of experimentally induced rat glomerulonephritis (2007)
  • Author(s): Kudo Y, Kakinuma Y, Iguchi M, Sato T, Sugiura T, Furihata M, Shuin T.
  • Journal Title: Nephron Exp Nephrol 106
  • Peer Reviewed
• [Journal Article] WT1 (Wilms'tumor 1)peptide immunotherapy for renal cell carcinoma (2007)
  • Author(s): Iiyama T, Udaka K, Takeda S, Takeuchi T, Adachi YC, Ohtsuki Y, Tsuboi A, Nakatsuka S, Elisseeva OA, Oji Y, Kawakami M, Nakajima H, Nishida S, Shirakata T, Oka Y, Shuin T, Sugiyama H.
  • Journal Title: Microbiol Immunol 51 Pages: 519-530
  • Peer Reviewed
• [Journal Article] Ethanol consumption alters expression and colocalization of bile salt export pump and multidrug resistance protein 2 in the rat (2007)
  • Author(s): Zinchuk V, Zinchuk O, Akimaru K, Moriya F, Okada T.
  • Journal Title: Histochem Cell Biol 127 Pages: 503-512
  • Peer Reviewed
• [Presentation] 腎細胞癌株を用いたHsp27アンチセンスの抗腫瘍効果 (2007)
  • Author(s): 鎌田 雅行, 川田 千明, 安岡 香織, 井上 啓史, 執印 太郎, GleaveME
  • Organizer: 日本癌治療学会
  • Place of Presentation: 京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Antitumor effect assessement of Hsp27 antisence using kidney tumor cell Line (2007)
  • Author(s): M, Kamada, C, Kawada, K, Yasuoka, K, Inoue, T, Shuin, GleaveME
  • Organizer: Japan Society of Clinical Oncology
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より