Detection and functional analysis in the genes associated with the development and differentiation of germ cells
Project/Area Number |
18591766
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Urology
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Research Institution | Nagoya City University |
Principal Investigator |
HAYASHI Yutaro Nagoya City University, Graduate School of Medical Sciences, Associate Professor (40238134)
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Co-Investigator(Kenkyū-buntansha) |
KOJIMA YOSHIYUKI Nagoya City University, Graduate School of Medical Sciences, Research Associate (60305539)
MARUYAMA Tetsuji Nagoya City University, Graduate School of Medical Sciences, Researcher (50305546)
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Project Period (FY) |
2006 – 2007
|
Project Status |
Completed (Fiscal Year 2007)
|
Budget Amount *help |
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
Keywords | ES cell / Primordial germ cell / Spermatogenesis / Subtraction / Glucose / 生殖細胞 / Subtraction法 / 培養条件 |
Research Abstract |
In a previous study, we established a system for visualizing the development of germ cells from mouse embryonic stem (ES) cells in culture using knock-in ES clones in which visual reporter genes were expressed from the mouse vasa homolog, Mvh. While assessing various culture conditions, we found that germ-cell formation was markedly depressed in low glucose medium. Using a repeated polymerase chain reaction (PCR) subtraction method, we identified genes that were differentially expressed in low versus high glucose media. Three genes that were predominantly expressed in high glucose medium, thioredoxin-interacting protein (Txnip), pituitary tumor-trans forming gene 1 (Pttg), and RuvB-like protein 2 (RuvB12), were further investigated. These genes were also found to be highly expressed in adult and embryonic gonads, and RuvB12 in particular, which encodes an ATP-dependent DNA helicase, was specifically detected in the spermatocytes and spermatids of the adult testis as well as in primordial germ cells. Furthermore, using a green fluorescent protein (GFP) fusion construct, we found that RuvBl2 was expressed in both the nucleus and cytoplasm of testicular germ cells. These findings suggest a possible relationship between glucose metabolism and germ-cell development.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Management of urethral calculi associated with hairballs after urethroplasty for severe hypospadias2007
Author(s)
Havashi, Y, Yasui, T, Koiima, Y, Maruvama, T, Tozawa, K, Kohri, K
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Journal Title
Int J Urol 14
Pages: 161-3
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ventral based dartos flap for the prevention of the urethrocutaneous fistula urethroplasty2007
Author(s)
Hayashi, Y, Kojima, Y, Nakane, A, Kurokawa, S, Tozawa, K, Kohri, K
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Journal Title
Int J Ural 14
Pages: 725-8
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genes associated with the formation of germ cells from embryonic stem cells in cultures containing different glucose concentrations2006
Author(s)
Mizuno, K, Tokumasu, A, Nakamura, A, Havashi, Y, Kojima, Y, Kohri, K, Noce, T
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Journal Title
Mol Reprod Dev 73
Pages: 437-45
Description
「研究成果報告書概要(欧文)」より
Related Report
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