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The effect of steroidogenesis and gonadal function in microgravity (The determination of responsible gene)

Research Project

Project/Area Number 18591768
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNagoya City University

Principal Investigator

KANEKO Tomoyoshi  Nagoya City University, Graduate School of Medical Sciences, Researcher (10381823)

Co-Investigator(Kenkyū-buntansha) SASAK Shoichi  I Nagoya City University, Graduate School of Medical Sciences, Assistant Professor (50225869)
KOJIMA Yoshiyuki  Nagoya City University, Graduate School of Medical Sciences, Research Associate (60305539)
IKEUCHI Takahito  Nagoya City University, Graduate School of Medical Sciences, Researcher (90315888)
KOHRI Kenjiro  Nagoya City University, Graduate School of Medical Sciences, Professor (30122047)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,850,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSimulated conditions of microgravity / Steroid production / Clinostat Rotation / TM3 cell line / I-10 cell line / Space world / Testicular function / 擬似微小重力環境 / 造成機能
Research Abstract

TM3 cell as mouse leydig cell line and I-10 cells as mouse leydig tumor cell line were cultured for 24 hours under a simulated μG environment. Testosterone production was evaluated by EIA kitand Progesterone production was evaluated by radioimmunoassay as steroidogenesis.
The μG environment was simulated using clinostat rotation (CR). Rotational (Cont-1) and stationary (Cont-2) controls always received a regular gravity effect with and without rotatory motion, respectively.
Testosterone levels in cells under CR were decreased compared with those of both controls.
Progesterone levels in cells under CR were significantly increased compared with those of both controls. Increased. progesterone production was not related to the expression of either of the transcription factors (Ad4BP/SF-1 and DAX-1) or the enzyme (3BHSD). Although the cytoplasm expanded, the the number of viable cells, the rate of apoptosis and the amount of cAMP produced under CR did not significantly differ from that produced under both control environments.
We hypothesize that the cell itcelf may be affected by microgravity and a increase in progesterone levels under microgravity produced by the I-10 cells in the clinostat rotation may be due to the expansion of cytoplasm, where steroidogenesis occurs.
Gonadal function may be affected by cosmic radiation, acceleration, altered atmospheric pressure, or unknown factors besides gravity. More extended experiments in a space environment will be necessary to determine how microgravity affects the cycle of steroidogenesis.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (5 results)

All 2008 2007

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] 擬似微小重力環境ではLeydig腫瘍細胞のcell lineであるI-10細胞のプロゲステロン産生が充進する2008

    • Author(s)
      金子 朋功
    • Journal Title

      International Journal of Urology 15巻No.3

      Pages: 245-250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] SIMULATED CONDITIONS OF MICROGRAVITY INCREASES PROGESTERONE PRODUCTION IN I-10 CELLS OF LEYDIG TUMOR CELL LINE2008

    • Author(s)
      TOMOYOSHI, KANEKO, SHOICHI, SASAKI, YUKIHIRO, UMEMOTO, YOSHIYUKI, KOJIMA, TAKAHITO, IKEUCHI, KENJIRO, KOHRI
    • Journal Title

      International Journal of Urology (15)3

      Pages: 245-250

    • NAID

      10021277148

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 擬似微小重力環境ではLeydig腫瘍細胞のcell lineであるI-10細胞のプロゲステロン産生が亢進する2008

    • Author(s)
      金子 朋功
    • Journal Title

      International Journal of Urology 15(3)

      Pages: 245-250

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 微少重力環境がLeydig細胞のステロイド合成に与える影響2007

    • Author(s)
      金子 朋功
    • Organizer
      第95回日本泌尿器科学会総会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2007-04-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] The effect of steroidogenesis of leydig cell in microgravity2007

    • Author(s)
      Tomoyoshi, Kaneko
    • Organizer
      95th The Japanese urological association
    • Place of Presentation
      Kobe international conference hall
    • Year and Date
      2007-04-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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