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Development of the prediction system for chemosensitivity of Methotrexate, Vinblastine, Doxorubicin, and Cisplatin neoadjuvant chemotherapy in invasive bladder cancer patients

Research Project

Project/Area Number 18591769
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionIwate Medical University

Principal Investigator

FUJIOKA Tomoaki  Iwate Medical University, School of Medicine, Professor (80173409)

Co-Investigator(Kenkyū-buntansha) SUGIMURA Jun  Iwate Medical University, School of Medicine, Lecturer (80306018)
OBARA Wataru  Iwate Medical University, School of Medicine, Lecturer (90337155)
RYO Takata  Iwate Medical University, School of Medicine, Professor (00438467)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,980,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsBladder Cancer / MVAC chemotherapy / Prediction of Chemosensitivity / Gene expression profiles / Personalized medicine / Real-time PCR / M-VAC化学療法 / 術前化学療法 / 個別化医療 / C-VAC療法
Research Abstract

To predict the efficacy of the M-VAC neoadjuvant chemotherapy for invasive bladder cancers, we previously established the method to calculate the prediction score on the basis of expression profiles of 14 predictive genes. This time, we constructed the prediction system using handy, cheap, real-time PCR method for a clinical application. First, we designed primers and probes that were able to detect 14 genes that strongly related to response of MVAC chemotherapy. Afterwards, expression level of each gene was analyzed by real-time PCR We used CCT6A as internal control. The obtained level of the gene expression was highly correlated with that of microarrays. We did quantitative real-time RT-CR of the 14 predictive genes for 15 learning cases, and calculated the prediction score for each case. When we estimated these scores by the leave-one-out cross validation test, all cases were placed correctly according to their response to M-VAC. We showed further that the responses of test cases were also predicted with accuracy 'lb examine the possibility of adapting our prediction system for clinical use, we attempted to establish a perdiction "card" system using Taqman Low Density Aarray (TLDA; Applied biosystems). We examined the chemosensitivity of 38 cases with the card, and predicted accurately by the sensitivity of 80% or more. We can predict response within one week when we use this card, and technical burden will be reduced. At present, we began a clinical research employing our prediction system with the card. This research is expected to become the precursors of personalized medicine.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (44 results)

All 2008 2007 2006

All Journal Article (24 results) (of which Peer Reviewed: 4 results) Presentation (17 results) Book (3 results)

  • [Journal Article] 膀胱癌組織の遺伝子発現解析によるMVACネオアジュバント化学療法の効果予測2008

    • Author(s)
      高田 亮, 他
    • Journal Title

      Urology View 6

      Pages: 99-103

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prediction of chemosensitivity to MVAC neoadjuvant chemotherapy by gene expression analysis of bladder cancer tissue2008

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Urology View 6

      Pages: 99-103

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Validation study of the prediction system for clinical respo nse of M-VAC neoadjuvant chemotherapy2007

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Cancer Sci 98

      Pages: 113-117

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 進行性膀胱癌におけるMVAC術前化学療法の有効性診断法の開発2007

    • Author(s)
      高田 亮, 他
    • Journal Title

      実験医学 25

      Pages: 63-71

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] M-VAC術前補助化学療法感受性予測の臨床応用に向けた感受性予測カードシステムの構築2007

    • Author(s)
      高田 亮, 他
    • Journal Title

      泌尿器外科 20

      Pages: 543-545

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Validation study of the prediction system for clinical response of M-VAC neoadjuvant chemotherapy2007

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Cancer Sci 98(1)

      Pages: 113-117

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of the prediction system for clinical response of M-VAC neoadjuvant chemotherapy2007

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Experimental Medicine 25

      Pages: 63-71

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development for clinical use of prediction system to M-VAC neoadjuvant chemotherapy2007

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Hinyoki Geka 20

      Pages: 543-545

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] M-VA.C術前補助化学療法感受性予測の臨床応用に向けた感受性予測カードシステムの構纂2007

    • Author(s)
      高田 亮, 他
    • Journal Title

      泌尿器外科 20

      Pages: 543-545

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Molecular Features of Hormone-Refractory Prostate Cancer Cells by Genome-Wide Gene Expression Profiles.2007

    • Author(s)
      Tamura, K, Furihata, M., Tsunoda, T., Ashida, S., Takata, R., et. al.
    • Journal Title

      Cancer research 67

      Pages: 5117-5125

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Features of Hormone-Refractory Prostate Cancer Cells by Genome-Wide Gene Expression Profiles.2007

    • Author(s)
      Kanehira, M., Harada, Y., Takata, R., et. al.
    • Journal Title

      Oncogene 26

      Pages: 6448-6445

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 進行性膀胱癌におけるM-VAC術前化学療法の有効性診断法の開発2007

    • Author(s)
      高田 亮, 他
    • Journal Title

      実験医学 25

      Pages: 63-71

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Oncogenic Role of MPHOSPH1,a Cancer-Testis Antigen Specific to Human Bladder Cancer2007

    • Author(s)
      Kanehira, M., Katagiri, T., Shimo, A., Takata, R., et. al.
    • Journal Title

      Cancer research 67

      Pages: 3276-3285

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oncogenic role of MPHOSPH1, a cancer-testis antigen specific to human bladder cancer2007

    • Author(s)
      Mitsugu Kanehira, et al.
    • Journal Title

      Cancer Research (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 患者の個性を考慮したオーダーメイド医療の実現にむけて-遣伝子発現情報を用いた浸潤性膀胱癌に対するM-VAC術前化学療法感受性予測システムの構築-2006

    • Author(s)
      高由 亮, 他
    • Journal Title

      岩手医誌 58

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] オーダーメイド医療に向けて2006

    • Author(s)
      藤岡 知昭, 他
    • Journal Title

      臨床泌尿器科 60

      Pages: 921-925

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 遺伝子発現情報に基づいた膀胱癌M-VAC術前科学療法感受性予測システムの開発およびその臨床応用2006

    • Author(s)
      高田 亮, 他
    • Journal Title

      泌尿器外科 19

      Pages: 349-352

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Association, The realization of "personalized medicine" prescribing the correct treatment regimen for each patient2006

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Journal of Iwate Medical 58(1)

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Aim for the realization of a personalized medicine2006

    • Author(s)
      Fujioka, T., et. al.
    • Journal Title

      Rinsho Hinyokika 60

      Pages: 921-925

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Predicting response to M-VAC neoadjuvant chemotherapy for bladder cancers through genome-wide gene expression profiling2006

    • Author(s)
      Takata, R., et. al.
    • Journal Title

      Hinyoki Geka 19

      Pages: 349-352

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Validation study of the prediction system for clinical response of M-VAC neoadjuvant chemotherapy2006

    • Author(s)
      Ryo Takata, et al.
    • Journal Title

      Cancer Science 98・1

      Pages: 113-117

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 患者の個性を考慮したオーダーメイド医療の実現に向けて-遺伝子発現情報を用いた浸潤性膀胱癌におけるM-VAC術前化学療法感受性予測システムの構築-2006

    • Author(s)
      高田 亮, 他
    • Journal Title

      岩手医学雑誌 58・1

      Pages: 1-8

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Eight Year Experience with Suder Ileal Neobladder.2006

    • Author(s)
      Wataru Obara, et al.
    • Journal Title

      Japanese Journal of Clinical Oncology 36・7

      Pages: 418-24

    • Related Report
      2006 Annual Research Report
  • [Journal Article] オーダーメイド医療に向けて2006

    • Author(s)
      藤岡 知昭, 他
    • Journal Title

      臨床泌尿器科 60・12

      Pages: 921-925

    • Related Report
      2006 Annual Research Report
  • [Presentation] Development of the prediction system for chemosensitivity of Methotrexate, Vmblastine, Doxorubicin, and Ci splatin neoadjuvant chemotherapy in invasive bladder cancer patients2007

    • Author(s)
      Takata R., et. al.
    • Organizer
      AUA 2007 annual meeting
    • Place of Presentation
      Anaheim,CA
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] M-VAC術前化学療法における感受性予測システムの確立-感受性予測による個別化医療は予後を改善しうるか2007

    • Author(s)
      高田 亮, 他
    • Organizer
      第95回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prognostic significance of prediction system for sensitivity of MVAC neo adjuvant chemotherapy in invasive bladder cancer2007

    • Author(s)
      Takata R., et. al.
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of the prediction system for chemosensitivity of Methotrexate, Vinblastine, Doxorubicin, and Cisplatin neoadjuvant chemotherapy in invasive bladder cancer patients2007

    • Author(s)
      Takata R., et. al.
    • Organizer
      AUA 2007 annual meeting
    • Place of Presentation
      Anaheim
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of prediction system in MVAC neoadjuvant chemotherapy -Can the personalized medicine by the prediction system improve the prognosis?-2007

    • Author(s)
      Takata R., et. al.
    • Organizer
      The 95th annual meeting of the Japanese urological association
    • Place of Presentation
      Kobe
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prognostic significance of prediction system for sensitivity of MVAC neoadjuvant chemotherapy in invasive bladder cancer2007

    • Author(s)
      Takata R., et. al.
    • Organizer
      The 66th annual meeting of the Japanese Cancer Association
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] M-VA.C術前化学療法における感受性予測システムの確立-感受性予測による個別化医療は予後を改善しうるか-2007

    • Author(s)
      高田 亮, 他
    • Organizer
      第95回日本泌尿器科学会総会
    • Place of Presentation
      神戸
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of the prediction system for chemosensitivity of methotrexate, vinblastin, doxor ubicin and cisplati meoasjucant chemotherapy in invasive bladder cancer patients.2007

    • Author(s)
      Takata, R., et. al.
    • Organizer
      AUA 2007 ANNUAL MEETING
    • Place of Presentation
      アナハイム、アメリカ
    • Related Report
      2007 Annual Research Report
  • [Presentation] Prognostic significance of prediction system for sensitivity of MVAC neoadjuvant chemotherapy in invasive bladder cancer.2007

    • Author(s)
      Takata, R., et. al.
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] Predicting response to M-VAC neoadjuvant chemotherapy for bladder cancers through genomewide gene expression profiling2006

    • Author(s)
      Takata R., et. al.
    • Organizer
      第101回米国泌尿器科学会
    • Place of Presentation
      Atlanta,Georgia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] M-VAC術前補助化学療法感受性予測の臨床対応に向けた感受性予測カードシステムの構築2006

    • Author(s)
      高田 亮, 他
    • Organizer
      第65回日本癌学会学術総会
    • Place of Presentation
      千葉
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 浸潤性膀胱癌に対するM-VAC術前化学療法の予後改善効果の検討2006

    • Author(s)
      高田 亮, 他
    • Organizer
      第44回日本癌治療学会総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 浸潤性膀胱癌に対するM-VA,C術前化学療法の予後改善効果の検討M-VAC術前補助化学療法感受性予測の臨床対応に向けた感受性予測カードシステムの構築2006

    • Author(s)
      高田 亮, 他
    • Organizer
      第71回日本泌尿器科学会東部総会
    • Place of Presentation
      東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Predicting response to M-VAC neoadjuvant chemotherapy for bladder cancers through genomewide gene expression profiling2006

    • Author(s)
      Takata R., et. al.
    • Organizer
      The 101st AUA annual meeting
    • Place of Presentation
      Atlanta
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Construction of prediction "card" system to predict response of M-VAC neoadjuvant chemotherapy for clinical application2006

    • Author(s)
      Takata R., et. al.
    • Organizer
      The 65th annual meeting of the Japanese Cancer Association
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Examination of effect of prognosis improvement of M-VAC neoadjuvant chemotherapy to invasive bladder cancer.2006

    • Author(s)
      Takata R., et. al.
    • Organizer
      The 44th Annual Meeting of the Japan Society of Clinical Oncology
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prognostic significance of prediction system for sensitivity of MVAC neoadjuvant chemotherapy in invasive bladder cancer2006

    • Author(s)
      Takata R., et. al.
    • Organizer
      The 95th annual meeting of Eastern section of the Japanese urological association
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 分子レベルから迫る癌診断研究〜臨床応用への挑戦〜2007

    • Author(s)
      中村 祐輔
    • Total Pages
      230
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 泌尿器疾患治療の新しいストラテジー2007

    • Author(s)
      村井 勝
    • Total Pages
      385
    • Publisher
      メジカルビュー社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 泌尿器疾患治療の新しいストラテジー2007

    • Author(s)
      村井 勝, 他
    • Total Pages
      385
    • Publisher
      メジカルビュー
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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