• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Determination of controlling factor of detrusor contraction in urothelium and the role of Rho Kinase

Research Project

Project/Area Number 18591770
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionDokkyo Medical University

Principal Investigator

YAMANISHI Tomonori  Dokkyo Medical University, Department of Urology, MD. Assoaate Professor of Department of Urology (90220425)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,950,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsbladder / urothelium / muscarinic / β-adrenoceotor / Y 27632 / fasudil / Rho / Rho-kinase / Y27632 / Fasudil / Rho活性 / カルバコール / 平滑筋 / サブタイプ / 下部尿路閉塞
Research Abstract

The mRNA and protein expression of muscarinic receptor subtypes, adorenoceptor subtypes as well as RhoA/Rho kinase and its activity were investigated by radioligand binding experiments, realtime RT-PCR, Western blotting and immunohistochemistry in urothelium and detrusor muscle of the pig and human. We have hind that M2 muscarinic, receptor subtypes were predominantly present in both urothelium and detrusor of the pig bladder. However M3 muscarinic receptor subtypes were more hind in urothelium and smooth muscle of the human bladder Among β-receptor subtypes, β3-adrenoceptor were predominantly found in detrusor muscle of the pig and human.
The expression of RhoA was more hind in the urothelium than in the smooth mule of the bladder in pig and human. The activity of RhoA determined by Western blotting were also greater in the urothelium than in the smooth muscle of the pig bladder.
In functional studies, the responses to Y-27632 and fasudil, Rho-kinase inhibitors, were examined in bladder … More tissue strips with or without urothelium. Mansion decrease after administration of Y-27632 (InM-100μM) was significantly greater in tissues with intact urothelium than in tissues without urothelium, after pre-contraction with KCl (decrease by 52.0±4.6% vs 28±6.8%, respectively; p=0.0088) or with carbachol (decrease by 53.1±7.2% vs 30.6±5.81%, respectively; p=0.0035). Maximum contraction on CRC to carbachol was reduced significantly alter administration of 3, 10 and 30μM Y-27632 (to 72.2±6.8%, 43.9±7.1%, and 25.015.5%, respectively of the control value) in strips with intact urothelium (n=36), but was reduced only at 10 and 30 μM (to 66.7±825% and 85.6±2.6%, respectively) in tissues without urothelium Inhibitory effect of Y-27632 (3- 30μM) on the response to electrical field stimulation at 20Hz and 50 Hz was also greater in tissue strips with intact urothelium than in tissues without urothelium.
In conclusion, the expression of RhoA mRNA and activated RhoA were greater in urothelium than in bladder smooth muscle. Y-27632 showed a stronger inhibitory effect on tissue preparations with intact urothelium than co those without urothelium. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (34 results)

All 2008 2007 2006 Other

All Journal Article (17 results) (of which Peer Reviewed: 7 results) Presentation (13 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Effectsd of 138-355,a β3-adzenoceptor selective agonist,on relazatiom of the human detrusor muade in vitro2007

    • Author(s)
      Yamanishi T, et. al.
    • Journal Title

      Nemvurology and Urodynamics 25

      Pages: 815-819

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Defning overactive bladder as byperseathtivity2007

    • Author(s)
      Yamaguchi O
    • Journal Title

      Neuromrology and Urodynamica 16

      Pages: 904-907

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Which β-adrenoceptor subtypes are important in the treatment of overactive bladder2007

    • Author(s)
      Yamanishi T, et. al.
    • Journal Title

      Cur Drug Ther 2

      Pages: 79-84

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Bandomizad,double-blind,sham-controlled evaluation of the effect of functional continuors magnetic stimnlatioa is patients with urgency incontinence2007

    • Author(s)
      Suzuki T, et. al.
    • Journal Title

      Neurourol Urodyn 26

      Pages: 767-72

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Which β-adrenoceptor subtypes are important in the treatment of overactive bladder?2007

    • Author(s)
      Yamanishi, T., et. al.
    • Journal Title

      Cur Drug Ther 2

      Pages: 79-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Randomized, double-blind, sham-controlled evaluation of the effect of functional continuous magnetic stimulation in patients with urgency incontinence2007

    • Author(s)
      Suzuki, T., et. al.
    • Journal Title

      Neurourol Urodyn 26

      Pages: 767-72

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Defining overactive bladder as hypersensitivity2007

    • Author(s)
      Yamaguchi, O., et. al.
    • Journal Title

      Neurourol Urodyn 16

      Pages: 904-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Defming overactive bladder as hypersensitivity2007

    • Author(s)
      Yamaguchi O
    • Journal Title

      Neurourology and Urodynamics 16

      Pages: 904-907

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Which β-adranocaptor subtypes are important in the treatment of overactive bladder2007

    • Author(s)
      Yamanishi T
    • Journal Title

      Cur Drug Ther 2

      Pages: 79-84

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Which β-adrenoceptor subtypes are important in the treatment of overactive bladder?2007

    • Author(s)
      Yamanishi T, et al.
    • Journal Title

      Cur Drug Ther 79

      Pages: 79-84

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of 138-355, a β3-adrenoceptorselective agonist, on relaxation of the human detrusor muscle in vitro.2007

    • Author(s)
      Yamanishi T, et al.
    • Journal Title

      Neurourol Urodyn 25

      Pages: 815-819

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 排尿筋収縮における尿踏上皮とRho-kinaseの役割2006

    • Author(s)
      山西 友典
    • Journal Title

      自律神経 43

      Pages: 315-319

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of 138-355, a(β3-adrenoceptor selective agonist, on relaxation of the human detrusor muscle in vitro2006

    • Author(s)
      Yamanishi, T., et. al.
    • Journal Title

      Neurourol Urodyn 25

      Pages: 815-819

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Urinary dysfunction and autonomic control in amyloid neuropathy.2006

    • Author(s)
      Ito T, et al.
    • Journal Title

      Clin Auton Res 16

      Pages: 66-71

    • Related Report
      2006 Annual Research Report
  • [Journal Article] incomplete emptying and urinary retention in multiple-system atrophy : When does it occur and how do we manage it?2006

    • Author(s)
      Ito T, et al.
    • Journal Title

      Mov Disord 21

      Pages: 816-823

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Practice patterns of Japanese physicians in urologic surveillance and management of spinal cord injury patients.2006

    • Author(s)
      Kitahara S, et al.
    • Journal Title

      Spinal Cord 44

      Pages: 362-368

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Symptom assesment tool for overactive bladder syndrome-overactive bladder symptom score.2006

    • Author(s)
      Hommma Y, et al.
    • Journal Title

      Urology 618

      Pages: 318-323

    • Related Report
      2006 Annual Research Report
  • [Presentation] ブタ膀胱Rho-kinaseの活性およびmRNAの発現2008

    • Author(s)
      水野 智弥
    • Organizer
      96回日本泌尿器科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2008-04-26
    • Related Report
      2007 Annual Research Report
  • [Presentation] Urodynamic effects of silodosin, a new alphalAlpha-adrenoceptor selective antagonist, for the treatment of benign prostatic hyperplasia2008

    • Author(s)
      Yamanishi, T., et. al.
    • Organizer
      23rd Annual European Association Urology Congress
    • Place of Presentation
      Milan
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Present advances in clinical aspects of neurogenic bladder(Symposium:The Neurogeaic Bladder:Where are we now?)2007

    • Author(s)
      Yamanishi T
    • Organizer
      2nd Pan-Pacific Continence Society Meeting
    • Place of Presentation
      Seoul
    • Year and Date
      2007-10-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Present advances in clinical aspects of neurogenic bladder.(Symposium : The Neurogenic Bladder : Where are we now?)2007

    • Author(s)
      Yamanishi, T
    • Organizer
      2nd Pan-Pacific Continence Society Meeting
    • Place of Presentation
      Seoul
    • Year and Date
      2007-10-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Lower urinary tract dysfunction after radical bysterectomy2007

    • Author(s)
      Yamanishi T, et. al.
    • Organizer
      22nd Annual Euvpean Urological Association Congress
    • Place of Presentation
      Berlin
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Lower urinary tract dysfunction after radical hysterectomy2007

    • Author(s)
      Yamanishi, T., et. al.
    • Organizer
      22nd Annual European Urological Association Congress
    • Place of Presentation
      Berlin
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Urodvnamic effects of silodosin,a new alphalAlpba- adrenoceptor selective antagonist,for the treatment of benign prostatic liyperplasia2006

    • Author(s)
      Yamanishi T, et. al.
    • Organizer
      23rd Annual European Association Urology Congress
    • Place of Presentation
      Milan
    • Year and Date
      2006-03-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The presence and the functional role of muscarinic receptor subtypes in human bladder smooth muscle in patients vrith normal and detrusor overactivity2006

    • Author(s)
      Yamanishi T
    • Organizer
      European Association of Urology Meeting
    • Place of Presentation
      Paris
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Randomized sham-controlled evaluation of functional continuous magnetic stimulattion with pelvic floor muscle training(PFMT) in patients with urinary incontinence2006

    • Author(s)
      Yamanishi T, et. al.
    • Organizer
      21st European Association of Urology meeting
    • Place of Presentation
      Paris
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The effects of toltemdine on carbachol-indnced contraction in human bladder smooth muscles in normal state and is detrusor overactivity associated with benign prostatic hpperplasig2006

    • Author(s)
      Yamanishi T, et. al.
    • Organizer
      36th Annual Meeting of International Continence Society
    • Place of Presentation
      Cbristrhmrch
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Randomized sham-controlled evaluation of functional continuous magnetic stimulation with pelvic floor muscle training(PFMT) in patients with urinary incontinence2006

    • Author(s)
      Yamanishi, T., et. al.
    • Organizer
      21st European Association of Urology meeting
    • Place of Presentation
      Paris
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The presence and the functional role of muscarinic receptor subtypes in human bladder smooth muscle in patients with normal and detrusor overactivity2006

    • Author(s)
      Yamanishi, T., et. al.
    • Organizer
      21st European Association of Urology meeting
    • Place of Presentation
      Paris
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The effects of tolterodine on carbachol-induced contraction in human bladder smooth muscles in normal state and in detrusor overactivity associated with benign prostatic hyperplasia2006

    • Author(s)
      Yamanishi, T., et. al.
    • Organizer
      36th Annual Meeting of International Continence Society
    • Place of Presentation
      Christchurch
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] よくわかって役に立つ排尿障害すべて2007

    • Author(s)
      山西 友典
    • Total Pages
      11
    • Publisher
      永井書店
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] よくわかって役に立つ排尿障害のすべて2007

    • Author(s)
      山西 友典
    • Total Pages
      11
    • Publisher
      永井書店
    • Related Report
      2007 Annual Research Report
  • [Book] 今日の治療指針2007

    • Author(s)
      山西友典
    • Total Pages
      4
    • Publisher
      医学書院
    • Related Report
      2006 Annual Research Report
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.dokkyomed.ac.jp/dep-m/uro/shoukai/index-shoukai01.html

    • Related Report
      2007 Final Research Report Summary

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi