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TT902(Staphylococcal nuclease domain-containing protein 1) as a potential biomarker for prostate cancer

Research Project

Project/Area Number 18591775
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionJikei University School of Medicine

Principal Investigator

KURUMA Hidetoshi  Jikei University School of Medicine, School of Medicine, Research Associate (80327329)

Co-Investigator(Kenkyū-buntansha) EGAWA Shin  The Jikei University, School of Medicine, Professor (60160347)
TAKAHASHI Hiroyuki  The Jikei University, School of Medicine, Assistant Professor (00246414)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordscancer / Protein / Biomarker / 蛋白質
Research Abstract

Using high molecular weight proteomic analysis, we previously showed that Staphylococcal nuclease domain-containing protein 1 (SND1) is highly expressed in recurrent androgen insensitive prostate cancer tissues. The objective of this study was to further characterize SND1 expression and to investigate its biological potential in prostate cancer. Radical prostatectomy specimens were obtained from 62 prostate cancer patients. Immunohistochemical staining patterns of SND1 were evaluated using an in-house polyclonal antibody. We confirmed expression of SND1 mRNA in prostate cancer cells using an in situ hybridization technique. To determine its biological functions, we investigated cell growth by MTT assay in vitro using siRNA treatment. SND1 was expressed in small granular structure patterns in cytoplasm in 60 of 62 prostate cancers (97%). It was highly expressed in prostate cancer and high grade prostatic intraepithelial neoplasm (HGPIN) compared with hyperplasia and normal epithelium (p<0.0001). Its expression was found to be more intense in cancers with higher grade (p=0.025) and higher prostate specific antigen (PSA,p=0.012), respectively, reflecting a more aggressive phenotype. SND1 mRNA was overexpressed in cancer cells, and the growth activity was suppressed in SND1 knocked down cells. In conclusion, SND1 expression intensity was found parallel to the dedifferentiation status of prostate cancer cells, thus reflecting a more aggressive phenotype. SND1 may have a pivotal role in cell growth in prostate cancer since its endogenous RNA interference regulated cell growth. SND1 may be a promising potential biomarker and therapeutic target for prostate cancer.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (16 results)

All 2008 2007 2006

All Journal Article (13 results) (of which Peer Reviewed: 5 results) Presentation (1 results) Book (2 results)

  • [Journal Article] The maximum tumour length in biopsy cores as a predictor of outcome after radical prostatectomy2008

    • Author(s)
      Hayashi N, et. al.
    • Journal Title

      BJU Int 101

      Pages: 175-180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The maximum tumour length in biopsy cores as a predictor of outcome after radical prostatectomy2008

    • Author(s)
      Hayashi, N., Urashima, M., Kuruma, H., et. al.
    • Journal Title

      BJU Int 101-2

      Pages: 175-180

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The maximum tumour length inbiopsy cores as a predictor of outcome after radical prostate ctomy.2008

    • Author(s)
      Hayashi N
    • Journal Title

      BJU Int. 2

      Pages: 175-180

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prostate specific antigen adjusted for total prostatic tumor volume as a predictor for outcome after radical prostatectomy2007

    • Author(s)
      Hayashi N, et. al.
    • Journal Title

      Prostate Cancer Prostatic Dis 10

      Pages: 60-65

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Prostate-specific antigen adjusted for total prostatic tumor volume as a predictor for outcome after radical prostatectomy2007

    • Author(s)
      Hayashi, N., Urashima, M., Ikemoto, I., Kuruma, H., et. al.
    • Journal Title

      Prostate Cancer Prostatic Dis 10-1

      Pages: 60-65

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Prostate-specific antigen adjusted for total prostatic tumor volume as apredictor for outcome after radical prostatectomy.2007

    • Author(s)
      Hayashi N
    • Journal Title

      Prostate Cancer Prostatic Dis. 1

      Pages: 60-65

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 血清プロテオミクスによる新規マーカーの探索2007

    • Author(s)
      車 英俊
    • Journal Title

      日本臨床 65

      Pages: 127-131

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Prostate-specific antigen adjusted for total prostatic tumor volume as a predictor for outcome after radical prostatectomy.2007

    • Author(s)
      Hayashi N, Urashima M, Ikemoto I, Kuruma H, Arai Y, Kuwao S, Baba S, Egawa S
    • Journal Title

      Prostate Cancer Prostatic Disease 10・1

      Pages: 60-65

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Computer simulated additional deep apical biopsy enhances cancer detection in palpably benign prostate gland2006

    • Author(s)
      Matsumoto K, et. al.
    • Journal Title

      Int J Urol 13

      Pages: 1290-1295

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Computer simulated additional deep apical biopsy enhances cancer detection in palpably benign prostate gland2006

    • Author(s)
      Matsumoto, K., Egawa, S., Satoh, T., Kuruma, H., et. al.
    • Journal Title

      Int J Urol 13-10

      Pages: 1290-1295

    • NAID

      10018302863

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Computer simulated additional deep apical biopsy enhances cancer detection in palpably benign prostate gland.2006

    • Author(s)
      Matsumoto K, Egawa S, Satoh T, Kuruma H, Yanagisawa N, Baba S
    • Journal Title

      International Journal of Urology 13・10

      Pages: 1290-5

    • NAID

      10018302863

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Ammonium acid urate urolithiasis in Japan.2006

    • Author(s)
      Kuruma H, Arakawa T, Kubo S, Hyodo T, Matsumoto K, Satoh T, Egawa S, Baba S
    • Journal Title

      International Journal of Urology 13・5

      Pages: 498-501

    • NAID

      10019170408

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Growth inhibition efficacy of an adcnovirus expressing dual therapeutic genes, wild-type p53, and anti-erbB2 ribozyme, against human bladder cancer cells.2006

    • Author(s)
      Irie A, Matsumoto K, Anderegg B, Kuruma H, Kashani-Sabet M, Scanlon KJ, Uchida T, Baba S
    • Journal Title

      Cancer Gene Therapy 13・3

      Pages: 298-305

    • Related Report
      2006 Annual Research Report
  • [Presentation] 新規前立腺癌マーカータンパク質TT902の組織学的発現解析2007

    • Author(s)
      車 英俊
    • Organizer
      日本泌尿器科学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2007-04-17
    • Related Report
      2007 Annual Research Report
  • [Book] Annual Review腎臓2008泌尿器科領域尿路悪性腫瘍のプロテオミクス2008

    • Author(s)
      車 英俊
    • Total Pages
      268
    • Publisher
      中外医学社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Annual Review 腎臓20082008

    • Author(s)
      御手洗 哲也
    • Total Pages
      268
    • Publisher
      中外医学社
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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