Analysis of networking of DNA repair in DNA repairdeficient mice with impaied spermatogenesis
| Project/Area Number |
18591782
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Tottori University |
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Principal Investigator |
NAKANE Hironobu Tottori University, Faculty of Medicine, assistant professor (10304205)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | DNA reapor deficient mice / genetical instability / spermatogenesis / networking of DNA repair |
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| Research Abstract |
We focused on the relationship between networking of DNA repair and DNA repair-deficient mice with impaired spermatogenesis.
DNA microarray analysis of testis in DNA repair deficient mice showed a different expression pattern of the base excision repair (BER), nucleotide excision repair (NER), translesion DNA synthesis (TLS), mismatch repair (MMR) compared to control. Now, we are confirming these different expression pattern by carrying out RT-PCR (reverse transcription PCR), immunostaining, western blotting, real-time PCR. In a preliminary study, we found the experimental condition that treated DNA repair deficient mice had abnormal spermatogenesis m their testes. So, we are trying to confirm these data, and carrying out further analysis.
In addition, we found that XPG deficient mice (NER defect) had immature testes and ovaries, than were cheking time-specific gene expression in these organs. As we also found specific stain of platinum-blue in transmission electron microscopy, we were analyzing testes in DNA repair-deficient mice by transmission electron microscopy with platinum-blue stain. We can understand the relationship between networking of DNA repair and DNA repair-deficient mice with impaired spermatogenesis with these experiment.
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Report
(3 results)
Research Products
(15 results)
